Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PKHYL_RS02020 Genome accession   NZ_AP019516
Coordinates   392198..393253 (-) Length   351 a.a.
NCBI ID   WP_071002368.1    Uniprot ID   -
Organism   Psychrobacter sp. KH172YL61     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 387198..398253
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PKHYL_RS01995 (PKHYL_04660) - 388100..389230 (+) 1131 WP_240365860.1 hypothetical protein -
  PKHYL_RS02000 (PKHYL_04670) - 389206..389583 (+) 378 WP_240365861.1 RnfH family protein -
  PKHYL_RS02005 (PKHYL_04680) bamE 389634..390053 (-) 420 WP_058024230.1 outer membrane protein assembly factor BamE -
  PKHYL_RS02010 (PKHYL_04690) fur 390382..390816 (+) 435 WP_058024231.1 ferric iron uptake transcriptional regulator -
  PKHYL_RS02015 - 390953..392077 (-) 1125 Protein_399 PilT/PilU family type 4a pilus ATPase -
  PKHYL_RS02020 (PKHYL_04730) pilT 392198..393253 (-) 1056 WP_071002368.1 type IV pilus twitching motility protein PilT Machinery gene
  PKHYL_RS02025 (PKHYL_04740) - 393618..394346 (+) 729 WP_093067942.1 YggS family pyridoxal phosphate-dependent enzyme -
  PKHYL_RS02030 (PKHYL_04750) - 394413..395549 (-) 1137 WP_240365862.1 hemolysin family protein -
  PKHYL_RS02035 (PKHYL_04760) - 396190..397881 (-) 1692 WP_223334572.1 NAD+ synthase -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39047.95 Da        Isoelectric Point: 7.0439

>NTDB_id=72637 PKHYL_RS02020 WP_071002368.1 392198..393253(-) (pilT) [Psychrobacter sp. KH172YL61]
MAEKNTLSIEDLLRFTVKHKASDLHISAGMPAMIRTDGEMTRINMPAMTHQVVHQLIYDIMNDKQRADFEEFFETDFSFE
IPNLARFRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGDVFKRVSDFKRGVVLVTGPTGSGKSTTLAAMINYINESRKEH
ILTIEDPIEFVHESKKSLINQREVHRDTLGFEPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAA
KTIDRVIDVFPAAEKDMIRAMLSESLQAVISQTLLRRQAGGRVAAHEIMLGTPAIRNLIRENKIAQMYSAIQTGAGEGMI
TLDQTLKNLVAKGTISKDVARPYAKQPEAFL

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=72637 PKHYL_RS02020 WP_071002368.1 392198..393253(-) (pilT) [Psychrobacter sp. KH172YL61]
ATGGCTGAAAAAAACACCTTAAGTATCGAAGACTTGCTACGTTTTACGGTCAAACATAAAGCATCGGATTTACATATTTC
AGCTGGCATGCCTGCGATGATTCGTACCGATGGTGAGATGACACGCATCAATATGCCTGCGATGACGCACCAAGTGGTTC
ATCAGCTCATTTATGACATTATGAACGACAAGCAGCGCGCTGATTTTGAAGAGTTCTTTGAGACAGATTTTTCTTTTGAG
ATTCCAAATTTGGCACGTTTTCGAGTCAATGCATTCAATCAAAATCGCGGCGCAGGCGCGGTATTTCGTACCATCCCTTC
TAAAGTATTGACCATGGAAGACTTAGGGATGGGCGACGTGTTCAAACGTGTCTCAGACTTCAAACGTGGCGTGGTGTTAG
TTACCGGCCCAACTGGTTCGGGTAAATCGACGACGCTAGCGGCCATGATTAATTATATTAACGAAAGTCGTAAAGAGCAT
ATTTTAACGATTGAAGATCCGATTGAATTTGTCCATGAATCGAAAAAAAGCCTGATTAACCAGCGTGAAGTGCACCGCGA
TACCTTAGGTTTTGAGCCTGCATTACGTTCGGCATTGCGAGAAGATCCAGATGTCATCTTGGTTGGTGAGTTACGTGACC
TTGAGACCATTCGTTTGGCACTGACTGCGGCCGAAACAGGACATTTAGTATTCGGCACTCTGCATACTAGCTCAGCCGCT
AAGACGATTGACCGTGTCATCGACGTATTCCCTGCCGCCGAAAAAGATATGATTCGTGCCATGCTATCGGAGTCACTACA
AGCCGTTATTTCACAAACCCTATTGCGTCGTCAAGCTGGTGGACGTGTGGCAGCTCATGAAATCATGCTAGGCACACCCG
CTATCCGTAACTTAATACGTGAAAACAAAATTGCTCAGATGTATTCTGCCATCCAAACGGGTGCAGGCGAAGGCATGATT
ACGCTTGATCAAACCTTAAAGAACTTGGTTGCAAAAGGCACCATCAGTAAAGATGTGGCTCGTCCTTATGCCAAGCAACC
TGAGGCATTTTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(17-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.42

98.291

0.781
  pilT Acinetobacter baumannii strain A118

79.42

98.291

0.781
  pilT Acinetobacter nosocomialis M2

79.13

98.291

0.778
  pilT Pseudomonas aeruginosa PAK

77.616

98.006

0.761
  pilT Pseudomonas stutzeri DSM 10701

77.326

98.006

0.758
  pilT Acinetobacter baylyi ADP1

76.453

98.006

0.749
  pilT Legionella pneumophila strain Lp02

71.512

98.006

0.701
  pilT Legionella pneumophila strain ERS1305867

71.512

98.006

0.701
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.389

93.732

0.641
  pilT Vibrio cholerae strain A1552

68.389

93.732

0.641
  pilT Neisseria meningitidis 8013

64.348

98.291

0.632
  pilT Neisseria gonorrhoeae MS11

63.768

98.291

0.627
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

98.291

0.499
  pilU Acinetobacter baylyi ADP1

41.691

97.721

0.407
  pilU Pseudomonas stutzeri DSM 10701

40.708

96.581

0.393
  pilU Vibrio cholerae strain A1552

42.724

92.023

0.393

Multiple sequence alignment