Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M2494_RS21390 Genome accession   NZ_AP019411
Coordinates   4775588..4776622 (+) Length   344 a.a.
NCBI ID   WP_003377212.1    Uniprot ID   A0A0K8LZU2
Organism   Pseudomonas syringae pv. actinidiae strain MAFF613020     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4770588..4781622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2494_RS21360 (KPSA1B_104357) rdgB 4770675..4771271 (-) 597 WP_003377218.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  M2494_RS21365 (KPSA1B_104358) metW 4771302..4771922 (-) 621 WP_003377217.1 methionine biosynthesis protein MetW -
  M2494_RS21370 (KPSA1B_104359) - 4771930..4773069 (-) 1140 WP_003377216.1 homoserine O-acetyltransferase -
  M2494_RS21375 (KPSA1B_104360) - 4773359..4773949 (-) 591 WP_003377215.1 YggT family protein -
  M2494_RS21380 (KPSA1B_104361) proC 4773975..4774793 (-) 819 WP_003377214.1 pyrroline-5-carboxylate reductase -
  M2494_RS21385 (KPSA1B_104362) - 4774846..4775532 (-) 687 WP_003377213.1 YggS family pyridoxal phosphate-dependent enzyme -
  M2494_RS21390 (KPSA1B_104363) pilT 4775588..4776622 (+) 1035 WP_003377212.1 type IV pilus twitching motility protein PilT Machinery gene
  M2494_RS21395 (KPSA1B_104364) - 4776714..4777352 (-) 639 WP_003377211.1 C40 family peptidase -
  M2494_RS21400 (KPSA1B_104365) - 4777645..4778049 (+) 405 WP_003377210.1 TM2 domain-containing protein -
  M2494_RS21405 (KPSA1B_104366) - 4778378..4779649 (-) 1272 WP_003377209.1 dihydroorotase -
  M2494_RS21410 (KPSA1B_104367) - 4779646..4780650 (-) 1005 WP_003377208.1 aspartate carbamoyltransferase catalytic subunit -
  M2494_RS21415 (KPSA1B_104368) pyrR 4780663..4781175 (-) 513 WP_003377207.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37963.79 Da        Isoelectric Point: 7.0227

>NTDB_id=72630 M2494_RS21390 WP_003377212.1 4775588..4776622(+) (pilT) [Pseudomonas syringae pv. actinidiae strain MAFF613020]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDAKEVKALIYDIMTDRQRQDFEERLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKILSMEDLGMGSVFRKITDVARGLILVTGPTGSGKSTTLAAMIDYLNCNKHHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAQEKSMIRSMLSESLHAVVSQALLKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSSIQTGGSLGMQTLDMCL
ADLVKRGLITRESARERAKVPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=72630 M2494_RS21390 WP_003377212.1 4775588..4776622(+) (pilT) [Pseudomonas syringae pv. actinidiae strain MAFF613020]
ATGGATATTACCGAGCTGCTGGCCTTCAGTGCCAAACAGGGCGCGTCGGACTTGCACCTCTCTGCAGGTCTGCCACCGAT
GATCCGCGTGGACGGCGATGTGCGGCGTATCAACCTGCCCCCGCTGGACGCCAAGGAGGTCAAGGCGCTGATCTACGACA
TCATGACCGACCGGCAGCGTCAGGATTTTGAAGAGCGCCTGGAAACCGACTTTTCGTTTGAAGTGCCGGGTGTCGCGCGG
TTTCGGGTCAACGCATTCAACCAGAATCGCGGTGCGGGCGCGGTATTCCGGACCATTCCGTCGAAAATCCTCAGCATGGA
AGACTTGGGGATGGGCAGTGTGTTTCGCAAGATTACCGACGTGGCGCGTGGCCTGATTCTGGTGACCGGGCCGACCGGTT
CAGGCAAGTCGACGACCCTGGCGGCGATGATCGACTACCTCAACTGCAACAAGCATCACCATATCCTGACCATCGAAGAC
CCTATCGAATTCGTTCACGAGTCCAAGAAGTGCCTTGTCAACCAGCGCGAGGTGCACCGTGACACGCTGGGGTTTTCCGA
AGCGCTGCGCTCGGCCCTGCGTGAGGACCCGGATGTGATTCTGGTCGGCGAAATGCGTGACCTGGAGACCATTCGTCTGG
CGCTGACTGCTGCCGAAACCGGCCACCTGGTATTTGGCACGCTGCACACCACCTCGGCGGCCAAGACCATCGACCGGATC
GTCGATGTGTTCCCGGCGCAGGAAAAGTCGATGATTCGCTCGATGCTGTCCGAGTCGCTGCACGCGGTTGTTTCGCAGGC
GCTGCTCAAGAAGGTCGGCGGTGGGCGCGTGGCAGCGCATGAAATCATGATGGGCACCCCGGCGATTCGTAACCTGATTC
GCGAGGACAAGGTGGCGCAGATGTATTCGTCCATTCAAACAGGCGGCTCATTGGGCATGCAGACGCTGGACATGTGCCTG
GCCGATCTGGTGAAACGCGGCCTGATTACCCGCGAAAGCGCTCGCGAGCGGGCCAAGGTGCCGGACAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K8LZU2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.663

100

0.887
  pilT Pseudomonas aeruginosa PAK

87.209

100

0.872
  pilT Acinetobacter nosocomialis M2

78.488

100

0.785
  pilT Acinetobacter baumannii D1279779

78.488

100

0.785
  pilT Acinetobacter baumannii strain A118

78.488

100

0.785
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Legionella pneumophila strain Lp02

73.964

98.256

0.727
  pilT Legionella pneumophila strain ERS1305867

73.964

98.256

0.727
  pilT Neisseria meningitidis 8013

67.941

98.837

0.672
  pilT Neisseria gonorrhoeae MS11

67.647

98.837

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.766

97.965

0.654
  pilT Vibrio cholerae strain A1552

66.766

97.965

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.623

100

0.538
  pilU Acinetobacter baylyi ADP1

41.329

100

0.416
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.384

0.41
  pilU Vibrio cholerae strain A1552

40.299

97.384

0.392

Multiple sequence alignment