Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PY718_RS12330 Genome accession   NZ_CP119011
Coordinates   2540281..2541261 (+) Length   326 a.a.
NCBI ID   WP_001596138.1    Uniprot ID   -
Organism   Escherichia coli strain CE0007a     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2535281..2546261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY718_RS12300 (PY718_12295) - 2535659..2536645 (-) 987 WP_000784010.1 TRAP transporter substrate-binding protein -
  PY718_RS12305 (PY718_12300) hemW 2536958..2538094 (-) 1137 WP_000239947.1 radical SAM family heme chaperone HemW -
  PY718_RS12310 (PY718_12305) rdgB 2538087..2538680 (-) 594 WP_001174736.1 XTP/dITP diphosphatase -
  PY718_RS12315 (PY718_12310) yggU 2538688..2538978 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  PY718_RS12320 (PY718_12315) yggT 2538975..2539541 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  PY718_RS12325 (PY718_12320) yggS 2539559..2540263 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  PY718_RS12330 (PY718_12325) pilT 2540281..2541261 (+) 981 WP_001596138.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PY718_RS12335 (PY718_12330) ruvX 2541435..2541851 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  PY718_RS12340 (PY718_12335) yqgE 2541851..2542414 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  PY718_RS12345 (PY718_12340) gshB 2542523..2543473 (-) 951 WP_000593260.1 glutathione synthase -
  PY718_RS12350 (PY718_12345) rsmE 2543486..2544217 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PY718_RS12355 (PY718_12350) endA 2544297..2545004 (-) 708 WP_001305312.1 deoxyribonuclease I -
  PY718_RS12360 (PY718_12355) yggI 2545099..2545596 (-) 498 WP_032160294.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36057.30 Da        Isoelectric Point: 5.9983

>NTDB_id=724737 PY718_RS12330 WP_001596138.1 2540281..2541261(+) (pilT) [Escherichia coli strain CE0007a]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFEAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=724737 PY718_RS12330 WP_001596138.1 2540281..2541261(+) (pilT) [Escherichia coli strain CE0007a]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGAGGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAACAGCGGGAGATTGGTTTGCACTGTATGACTTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGTTGTTTGAATTGCTGATTAACACACCTGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362