Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M5T12_RS20185 Genome accession   NZ_CP103751
Coordinates   4099321..4100301 (-) Length   326 a.a.
NCBI ID   WP_023309128.1    Uniprot ID   A0AAQ0Y050
Organism   Enterobacter asburiae strain 2497     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4094321..4105301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5T12_RS20155 (M5T12_20155) - 4095197..4095694 (+) 498 WP_032667932.1 SprT family zinc-dependent metalloprotease -
  M5T12_RS20160 (M5T12_20160) endA 4095789..4096496 (+) 708 WP_023333343.1 deoxyribonuclease I -
  M5T12_RS20165 (M5T12_20165) rsmE 4096548..4097279 (+) 732 WP_023309126.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M5T12_RS20170 (M5T12_20170) gshB 4097299..4098246 (+) 948 WP_045355617.1 glutathione synthase -
  M5T12_RS20175 (M5T12_20175) - 4098334..4098894 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  M5T12_RS20180 (M5T12_20180) ruvX 4098894..4099310 (+) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  M5T12_RS20185 (M5T12_20185) pilT 4099321..4100301 (-) 981 WP_023309128.1 type IV pilus twitching motility protein PilT Machinery gene
  M5T12_RS20190 (M5T12_20190) - 4100319..4101020 (+) 702 WP_045355618.1 YggS family pyridoxal phosphate-dependent enzyme -
  M5T12_RS20195 (M5T12_20195) - 4101042..4101608 (+) 567 WP_023309130.1 YggT family protein -
  M5T12_RS20200 (M5T12_20200) yggU 4101605..4101901 (+) 297 WP_023309131.1 DUF167 family protein YggU -
  M5T12_RS20205 (M5T12_20205) - 4101905..4102498 (+) 594 WP_045355619.1 XTP/dITP diphosphatase -
  M5T12_RS20210 (M5T12_20210) hemW 4102491..4103633 (+) 1143 WP_045355620.1 radical SAM family heme chaperone HemW -
  M5T12_RS20215 (M5T12_20215) - 4103697..4104059 (+) 363 WP_071949300.1 endonuclease domain-containing protein -
  M5T12_RS20220 (M5T12_20220) - 4104135..4104851 (-) 717 WP_023309135.1 DUF2884 domain-containing protein -
  M5T12_RS20225 (M5T12_20225) - 4104908..4105234 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35521.53 Da        Isoelectric Point: 6.9108

>NTDB_id=724499 M5T12_RS20185 WP_023309128.1 4099321..4100301(-) (pilT) [Enterobacter asburiae strain 2497]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGIWWAKGQVDFASTVTGGQRLRG
SAFKQMRGVSVTLRLLPRSCPQLSSLGAPRAIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLGGSLRAVLAQRLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=724499 M5T12_RS20185 WP_023309128.1 4099321..4100301(-) (pilT) [Enterobacter asburiae strain 2497]
ATGGATGTGGAAGAAATTGTGGCTCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATTAGGTCGGCTTGAACCCGCTCCTTTTCCGCCTCCCGATGTCGGAGCGTTATTGAAAGCGTGGCTCAACG
ATGAGCAGCAGGGGATATGGTGGGCAAAGGGGCAGGTGGATTTTGCCTCGACGGTGACGGGAGGCCAGCGGCTGCGCGGC
AGTGCCTTTAAGCAGATGAGAGGTGTCTCTGTGACGCTGCGGCTGCTGCCGCGTAGCTGCCCACAGCTCTCTTCGCTGGG
CGCGCCGCGGGCGATCCCGGAACTGTTGTCCAATGACGCCGGGCTGATTCTGGTCACGGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTCAACCACCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAACGTTGCTTGATCCAGCAGCGGGAGATAGGTCAGCACAGCCCGTCATTTGCTGAAGCGCTGCG
CAGCGCCTTACGCCAGGATCCGGACGTTATTCTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGACACCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGGCGGCAGCCTGCGAGCGGTGCTGGCGCAGAGGCTGCTTCC
CGATCTGCAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACAGCGGCGGCGGCGAATCTGATTCGCGAAGGGA
AAACGTGGCAATTGCCCGGGATCATTCAAACCGGTCAGCAGGCAGGCATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Legionella pneumophila strain Lp02

49.379

98.773

0.488
  pilT Legionella pneumophila strain ERS1305867

49.379

98.773

0.488
  pilT Acinetobacter baylyi ADP1

48.318

100

0.485
  pilT Acinetobacter baumannii D1279779

48.318

100

0.485
  pilT Acinetobacter baumannii strain A118

48.318

100

0.485
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter nosocomialis M2

48.75

98.16

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.478

98.773

0.429
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362