NTDB

Overview

Name	pilT	Type	Machinery gene
Locus tag	PY720_RS05035	Genome accession	NZ_CP119000
Coordinates	1056328..1057308 (-)	Length	326 a.a.
NCBI ID	WP_094249975.1	Uniprot ID	-
Organism	Escherichia coli strain CE0026f
Function	type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1051328..1062308

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PY720_RS05005 (PY720_05005)	yggI	1052004..1052501 (+)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-
PY720_RS05010 (PY720_05010)	endA	1052596..1053303 (+)	708	WP_000286500.1	deoxyribonuclease I	-
PY720_RS05015 (PY720_05015)	rsmE	1053383..1054114 (+)	732	WP_001222508.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
PY720_RS05020 (PY720_05020)	gshB	1054127..1055077 (+)	951	WP_000593273.1	glutathione synthase	-
PY720_RS05025 (PY720_05025)	yqgE	1055186..1055749 (+)	564	WP_001053178.1	YqgE/AlgH family protein	-
PY720_RS05030 (PY720_05030)	ruvX	1055749..1056165 (+)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
PY720_RS05035 (PY720_05035)	pilT	1056328..1057308 (-)	981	WP_094249975.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PY720_RS05040 (PY720_05040)	yggS	1057326..1058030 (+)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
PY720_RS05045 (PY720_05045)	yggT	1058048..1058614 (+)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
PY720_RS05050 (PY720_05050)	yggU	1058611..1058901 (+)	291	WP_001277222.1	DUF167 family protein YggU	-
PY720_RS05055 (PY720_05055)	rdgB	1058909..1059502 (+)	594	WP_001174743.1	XTP/dITP diphosphatase	-
PY720_RS05060 (PY720_05060)	hemW	1059495..1060631 (+)	1137	WP_000239928.1	radical SAM family heme chaperone HemW	-
PY720_RS05065 (PY720_05065)	yggM	1060786..1061793 (-)	1008	WP_094249976.1	DUF1202 family protein	-

Sequence

Protein

Download Length: 326 a.a. Molecular weight: 35830.05 Da Isoelectric Point: 5.3850

                                >NTDB_id=724372 PY720_RS05035 WP_094249975.1 1056328..1057308(-) (pilT) [Escherichia coli strain CE0026f]

MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG

SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE

YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS

FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGCVALFELLINTPAVGNLILEGKTHQLPHVIQTGQQVGMITFQQSFQQR

VGEGRL

Nucleotide

Download Length: 981 bp

                                >NTDB_id=724372 PY720_RS05035 WP_094249975.1 1056328..1057308(-) (pilT) [Escherichia coli strain CE0026f]

ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG

ATGGCGCATTCGTGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG

ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC

AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG

TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT

CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA

TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACTTTCGCATCGGGGTTGCG

GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTAACGG

CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA

TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT

GGATAAACAGGAAGGATGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCTCGAAGGGA

AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTTTCAGCAGCGA

GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	48.93	100	0.491
pilT	Vibrio cholerae strain A1552	48.93	100	0.491
pilT	Neisseria meningitidis 8013	48.171	100	0.485
pilT	Neisseria gonorrhoeae MS11	47.866	100	0.482
pilT	Acinetobacter baylyi ADP1	46.177	100	0.463
pilT	Acinetobacter baumannii D1279779	45.872	100	0.46
pilT	Acinetobacter nosocomialis M2	45.872	100	0.46
pilT	Acinetobacter baumannii strain A118	45.872	100	0.46
pilT	Pseudomonas stutzeri DSM 10701	45.872	100	0.46
pilT	Pseudomonas aeruginosa PAK	45.566	100	0.457
pilT	Legionella pneumophila strain ERS1305867	44.648	100	0.448
pilT	Legionella pneumophila strain Lp02	44.648	100	0.448
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.509	97.546	0.405
pilU	Vibrio cholerae strain A1552	38.906	100	0.393
pilU	Pseudomonas stutzeri DSM 10701	36.97	100	0.374
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362

Detailed information NTG0724372