Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   myaer102_RS21390 Genome accession   NZ_AP019314
Coordinates   4168861..4169961 (+) Length   366 a.a.
NCBI ID   WP_002797770.1    Uniprot ID   I4HVR1
Organism   Microcystis viridis NIES-102     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4163861..4174961
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  myaer102_RS21375 (myaer102_39020) - 4164693..4165361 (+) 669 WP_002797767.1 HAD-IA family hydrolase -
  myaer102_RS21380 (myaer102_39030) grpE 4165451..4166173 (-) 723 WP_012264074.1 nucleotide exchange factor GrpE -
  myaer102_RS21385 (myaer102_39040) pilB/pilB1 4166700..4168715 (+) 2016 WP_125731554.1 GspE/PulE family protein Machinery gene
  myaer102_RS21390 (myaer102_39050) pilT 4168861..4169961 (+) 1101 WP_002797770.1 type IV pilus twitching motility protein PilT Machinery gene
  myaer102_RS32640 - 4170353..4170642 (+) 290 Protein_4037 type II secretion system F family protein -
  myaer102_RS32645 pilC 4170898..4171341 (+) 444 WP_232023877.1 type II secretion system F family protein Machinery gene
  myaer102_RS21400 - 4171437..4171646 (+) 210 WP_012264077.1 hypothetical protein -
  myaer102_RS21405 (myaer102_39080) - 4171725..4172192 (+) 468 Protein_4040 hypothetical protein -
  myaer102_RS21410 (myaer102_39090) - 4172235..4174805 (-) 2571 WP_002797773.1 TIGR03960 family B12-binding radical SAM protein -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 40304.51 Da        Isoelectric Point: 7.8147

>NTDB_id=72410 myaer102_RS21390 WP_002797770.1 4168861..4169961(+) (pilT) [Microcystis viridis NIES-102]
MELMIEDLMEQLVEMGGSDMHIQSGAPVYFRISGKLSPINEESLTPQDCQKLIFSMLNNTQRKELEQNWELDCSYGVKGL
ARFRVNVYKERGAYAACLRALSSKIPNFEQLNLPRVVRELSERPRGLILVTGQTGSGKTTTLAAILDLINRTRSEHILTV
EDPIEYVFPNIKSLFHQRQKGEDTKSFANALRASLREDPDIILVGEMRDLETISLAITAAETGHLVFGTLHTSSAAGTVD
RIIDVFPANQQAQIRAMLSNSLLAVFSQNLVKKKNPKPGEFGRAMVQEIMVVTPAIANLIREGKAPQVYSAIQTGMKLGM
QTMEQGLANLVVRGVVSLEEAISKSGKPDELQRLIAGAGANAKARI

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=72410 myaer102_RS21390 WP_002797770.1 4168861..4169961(+) (pilT) [Microcystis viridis NIES-102]
ATGGAATTAATGATCGAAGATTTAATGGAACAATTGGTCGAGATGGGCGGTTCCGATATGCACATTCAGTCTGGGGCGCC
GGTCTATTTTCGCATTAGTGGCAAACTGTCCCCGATTAATGAAGAATCTTTAACCCCGCAAGATTGTCAGAAATTGATTT
TTAGTATGCTCAATAATACCCAAAGAAAAGAGTTAGAGCAGAATTGGGAATTAGACTGTTCCTACGGGGTGAAAGGATTA
GCAAGATTTAGGGTCAATGTTTATAAAGAAAGGGGTGCTTATGCAGCTTGTTTACGGGCTTTGTCCTCTAAAATTCCTAA
CTTTGAACAATTAAATTTACCTAGAGTAGTACGGGAGTTATCGGAAAGACCGAGGGGATTAATTCTCGTAACTGGCCAAA
CAGGTTCAGGTAAAACTACCACTTTAGCGGCAATTTTAGACTTAATTAATCGGACTAGGTCTGAACATATTTTAACTGTA
GAAGACCCAATTGAATACGTTTTTCCTAATATAAAAAGTCTGTTTCACCAACGACAAAAAGGAGAAGATACCAAGAGTTT
TGCTAACGCTTTAAGAGCATCTTTACGGGAAGATCCTGATATTATCCTTGTGGGAGAAATGCGGGATTTAGAAACAATTA
GTTTGGCAATCACGGCGGCAGAAACTGGTCACTTAGTCTTCGGAACCCTACACACTAGCTCGGCAGCGGGAACAGTGGAT
CGGATTATTGACGTTTTTCCTGCTAACCAACAGGCACAAATTCGCGCCATGTTATCCAATTCTTTACTAGCAGTTTTTAG
TCAAAACCTAGTCAAGAAAAAAAATCCTAAACCGGGGGAATTTGGTCGGGCAATGGTACAGGAAATCATGGTGGTAACTC
CAGCGATCGCTAACCTAATTCGGGAAGGAAAAGCCCCCCAAGTCTATTCAGCAATTCAAACGGGAATGAAGTTAGGAATG
CAAACCATGGAACAGGGATTAGCTAATTTAGTTGTTAGGGGTGTTGTCTCTTTGGAAGAGGCCATTTCTAAAAGCGGTAA
ACCCGATGAATTACAACGTCTTATCGCCGGGGCCGGCGCTAACGCTAAAGCCCGGATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I4HVR1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

52.174

94.262

0.492
  pilT Legionella pneumophila strain ERS1305867

52.174

94.262

0.492
  pilT Pseudomonas aeruginosa PAK

51.297

94.809

0.486
  pilT Acinetobacter baumannii D1279779

51.009

94.809

0.484
  pilT Acinetobacter nosocomialis M2

51.009

94.809

0.484
  pilT Acinetobacter baumannii strain A118

51.009

94.809

0.484
  pilT Acinetobacter baylyi ADP1

50.432

94.809

0.478
  pilT Pseudomonas stutzeri DSM 10701

50.432

94.809

0.478
  pilT Vibrio cholerae strain A1552

50.882

92.896

0.473
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.882

92.896

0.473
  pilT Neisseria meningitidis 8013

49.263

92.623

0.456
  pilT Neisseria gonorrhoeae MS11

48.968

92.623

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.506

97.268

0.443
  pilU Pseudomonas stutzeri DSM 10701

38.462

95.902

0.369

Multiple sequence alignment