Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   myaer102_RS21385 Genome accession   NZ_AP019314
Coordinates   4166700..4168715 (+) Length   671 a.a.
NCBI ID   WP_125731554.1    Uniprot ID   -
Organism   Microcystis viridis NIES-102     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4161700..4173715
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  myaer102_RS21360 (myaer102_38990) rnpA 4161764..4162126 (-) 363 WP_002797764.1 ribonuclease P protein component -
  myaer102_RS21365 (myaer102_39000) rpmH 4162153..4162290 (-) 138 WP_002739276.1 50S ribosomal protein L34 -
  myaer102_RS21370 (myaer102_39010) - 4162381..4164345 (+) 1965 WP_125731552.1 amylo-alpha-1,6-glucosidase -
  myaer102_RS21375 (myaer102_39020) - 4164693..4165361 (+) 669 WP_002797767.1 HAD-IA family hydrolase -
  myaer102_RS21380 (myaer102_39030) grpE 4165451..4166173 (-) 723 WP_012264074.1 nucleotide exchange factor GrpE -
  myaer102_RS21385 (myaer102_39040) pilB/pilB1 4166700..4168715 (+) 2016 WP_125731554.1 GspE/PulE family protein Machinery gene
  myaer102_RS21390 (myaer102_39050) pilT 4168861..4169961 (+) 1101 WP_002797770.1 type IV pilus twitching motility protein PilT Machinery gene
  myaer102_RS32640 - 4170353..4170642 (+) 290 Protein_4037 type II secretion system F family protein -
  myaer102_RS32645 pilC 4170898..4171341 (+) 444 WP_232023877.1 type II secretion system F family protein Machinery gene
  myaer102_RS21400 - 4171437..4171646 (+) 210 WP_012264077.1 hypothetical protein -
  myaer102_RS21405 (myaer102_39080) - 4171725..4172192 (+) 468 Protein_4040 hypothetical protein -

Sequence

Protein


Download         Length: 671 a.a.        Molecular weight: 74927.05 Da        Isoelectric Point: 6.6200

>NTDB_id=72409 myaer102_RS21385 WP_125731554.1 4166700..4168715(+) (pilB/pilB1) [Microcystis viridis NIES-102]
MTFSSKKTRAVALRNYFSPFGNKLVQSGYIGADQMQRALVETRKSGRSLVEILQQLTGRPLPPDLQRQYKKNQLFELKIL
YGVESIDPELTDVDGLEIARLIDSLIPIDLCRRYRLIPLRKIEGEPAAILIAMVDPDNLEATDDINRILRPRELGLQRLV
ITSEDYERLLEKFHSVQSELEQEKARLQKEKELAKLSDLTGIVSNIEVASGAANDEVADDLGEGEANQAPVINLVNKILA
KALQEGTSDIHIEPQEEFLKIRFRKDGVLHQAFEPLPKKITPAVTARFKIMADLDIAERRLPQDGKIRRLYQGRTVDFRV
NTLPSRYGEKVCLRILDNSATQLGLDKLITDQKTLQIVRDLASHPFGLLLVTGPTGSGKSTTLYSVLAERNHPGVNISTA
EDPIEYSLPGITQVQVIREKGMDFASILRAFMRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIARLD
KMGVEPFMISGSLLGVLAQRLVRRVCSQCRVAYNPSQGELARFGLAAANEREVTFYKANTLTLEEIQQARKQGNLCLKCS
GSGYKGRIGVYEVMQNSERLQQLIYQGATTDRIKETAVDEGMVTLLAYSLNLVREGYTTLEEVERVTFTDSGLEAELKAK
RKSGLICRGCRAELQPEWLDCPYCMTPRFSD

Nucleotide


Download         Length: 2016 bp        

>NTDB_id=72409 myaer102_RS21385 WP_125731554.1 4166700..4168715(+) (pilB/pilB1) [Microcystis viridis NIES-102]
ATGACATTTTCTTCCAAGAAAACCCGTGCCGTTGCCCTGCGCAATTACTTTTCGCCCTTTGGTAACAAGTTGGTGCAGTC
GGGCTATATCGGTGCCGACCAGATGCAACGGGCCTTAGTGGAAACACGGAAATCGGGACGATCGCTAGTAGAAATATTAC
AACAACTAACCGGGCGCCCTTTGCCCCCGGATTTACAGCGACAGTATAAGAAAAACCAGTTATTTGAACTAAAAATTCTC
TACGGAGTCGAATCGATCGACCCCGAATTAACCGATGTGGATGGACTGGAAATAGCCCGATTGATCGATTCTTTGATTCC
GATCGATCTTTGTCGTCGCTATCGTCTCATCCCCCTGCGAAAGATCGAAGGAGAGCCGGCTGCGATTTTGATAGCCATGG
TCGATCCCGACAACCTCGAGGCTACCGACGATATCAATCGTATCCTCCGCCCCAGGGAGTTAGGTTTACAGCGTTTGGTG
ATTACCAGCGAAGATTATGAAAGATTACTGGAAAAATTCCACTCGGTCCAATCGGAGCTAGAACAGGAAAAAGCCCGACT
GCAAAAAGAAAAAGAACTGGCAAAACTCTCGGATCTCACTGGTATTGTCAGCAATATTGAGGTGGCCTCTGGTGCTGCTA
ACGATGAAGTGGCCGATGATCTCGGAGAAGGGGAAGCTAATCAAGCTCCCGTCATCAACCTCGTCAATAAAATTCTAGCT
AAAGCCCTACAGGAAGGGACTTCCGATATTCACATCGAACCCCAAGAAGAATTCCTGAAAATTCGCTTTCGCAAAGATGG
AGTTCTTCATCAAGCTTTTGAGCCACTGCCGAAAAAAATTACCCCGGCGGTCACTGCCCGTTTTAAAATCATGGCCGATC
TCGATATCGCCGAACGTCGTCTTCCCCAGGATGGTAAAATCCGGCGGCTTTATCAAGGGCGAACAGTGGATTTTCGGGTT
AATACCCTGCCCAGTCGCTACGGGGAAAAAGTCTGTTTACGGATTCTCGATAACTCGGCTACCCAGTTGGGTTTAGATAA
ACTGATCACCGATCAAAAGACCCTGCAAATCGTCCGAGACTTGGCCAGTCACCCTTTTGGTCTGTTACTGGTGACGGGCC
CCACCGGTTCGGGGAAATCCACCACTTTATACTCGGTGTTAGCGGAAAGAAATCACCCTGGAGTTAATATTAGTACCGCC
GAAGACCCGATCGAATATTCTCTACCAGGCATCACCCAGGTGCAGGTAATTCGGGAAAAAGGCATGGATTTCGCCTCGAT
TCTGCGGGCATTCATGCGACAAGATCCGGATGTGATTCTGGTGGGAGAAACTAGGGACAAGGAAACGGCTAAAACAGCGA
TCGAGGCCGCCCTAACTGGTCACTTGGTCCTCACTACCCTACACACCAACGATGCCGCTGGTGCGATCGCTCGTCTCGAT
AAGATGGGGGTTGAACCGTTTATGATTTCTGGTTCTCTCTTGGGAGTCTTGGCCCAGCGTCTCGTGCGGCGCGTCTGTAG
CCAATGTCGAGTCGCCTATAACCCCAGCCAAGGGGAATTAGCCCGTTTTGGTCTTGCCGCCGCCAATGAACGGGAAGTGA
CCTTTTATAAGGCTAATACCCTGACCCTAGAGGAAATTCAACAGGCGCGAAAACAGGGCAATCTCTGCCTTAAGTGTAGC
GGCAGCGGTTATAAAGGTCGCATTGGTGTCTATGAGGTAATGCAAAATAGTGAACGACTACAGCAGCTAATTTACCAAGG
GGCAACCACTGATCGCATTAAGGAGACGGCCGTCGATGAGGGCATGGTGACACTCCTAGCCTATAGTTTAAATCTGGTGC
GCGAGGGTTACACCACCCTCGAAGAAGTGGAACGGGTGACATTTACCGATTCCGGTCTGGAAGCGGAACTAAAAGCCAAA
CGGAAAAGCGGTTTAATCTGTCGTGGTTGTCGCGCCGAACTACAACCAGAATGGCTCGATTGTCCCTATTGTATGACACC
GCGATTTAGTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(237-504)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

72.508

98.659

0.715
  pilF Thermus thermophilus HB27

40.23

90.76

0.365

Multiple sequence alignment