Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M5S60_RS03805 Genome accession   NZ_CP103621
Coordinates   759777..760757 (+) Length   326 a.a.
NCBI ID   WP_004900568.1    Uniprot ID   A0AAJ3CQ32
Organism   Klebsiella pneumoniae strain 433     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 754777..765757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5S60_RS03770 (M5S60_03770) - 755313..755639 (+) 327 WP_002916620.1 YggL family protein -
  M5S60_RS03775 (M5S60_03775) - 755690..756409 (+) 720 WP_019704316.1 DUF2884 domain-containing protein -
  M5S60_RS03780 (M5S60_03780) hemW 756450..757586 (-) 1137 WP_002916617.1 radical SAM family heme chaperone HemW -
  M5S60_RS03785 (M5S60_03785) - 757579..758172 (-) 594 WP_032418358.1 XTP/dITP diphosphatase -
  M5S60_RS03790 (M5S60_03790) yggU 758185..758475 (-) 291 WP_002916614.1 DUF167 family protein YggU -
  M5S60_RS03795 (M5S60_03795) - 758472..759038 (-) 567 WP_002916613.1 YggT family protein -
  M5S60_RS03800 (M5S60_03800) - 759059..759760 (-) 702 WP_004144780.1 YggS family pyridoxal phosphate-dependent enzyme -
  M5S60_RS03805 (M5S60_03805) pilT 759777..760757 (+) 981 WP_004900568.1 type IV pilus twitching motility protein PilT Machinery gene
  M5S60_RS03810 (M5S60_03810) - 761106..761759 (+) 654 WP_002916607.1 LuxR family transcriptional regulator -
  M5S60_RS03815 (M5S60_03815) ruvX 761812..762228 (-) 417 WP_002916605.1 Holliday junction resolvase RuvX -
  M5S60_RS03820 (M5S60_03820) - 762228..762791 (-) 564 WP_002916603.1 YqgE/AlgH family protein -
  M5S60_RS03825 (M5S60_03825) gshB 762966..763916 (-) 951 WP_002916600.1 glutathione synthase -
  M5S60_RS03830 (M5S60_03830) rsmE 763937..764668 (-) 732 WP_032418360.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M5S60_RS03835 (M5S60_03835) endA 764760..765467 (-) 708 WP_002916589.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35283.76 Da        Isoelectric Point: 6.9959

>NTDB_id=723739 M5S60_RS03805 WP_004900568.1 759777..760757(+) (pilT) [Klebsiella pneumoniae strain 433]
MKLEEIVALSVKHNVSDLHLCNSAAPRWRRQGRLQPAPFPAPDIANLLNDWLDAAQLLHWQEHGQIDFALTLACGARLRA
SAFAHTRGISLVLRLLPEQCPRLDTLGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVGHLNQHLDGHILTLEDPVE
FIHHSERCLIQQREVGRHCPSFAAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVERLIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPAVANLIREGKVHQLPGVMQTGMQAGMLTFTQSFQQR
VAAGAL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=723739 M5S60_RS03805 WP_004900568.1 759777..760757(+) (pilT) [Klebsiella pneumoniae strain 433]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATTCCGCCGCACCGCG
CTGGCGGCGGCAGGGCAGGCTGCAGCCCGCGCCCTTTCCCGCGCCGGATATTGCAAATTTACTCAACGACTGGCTCGATG
CCGCACAGTTACTGCACTGGCAGGAACACGGCCAGATTGACTTTGCGCTGACCCTGGCCTGCGGTGCGCGGCTGCGCGCC
AGCGCCTTCGCCCATACGCGTGGGATATCGCTGGTGCTGCGCCTCCTTCCTGAACAGTGCCCGCGTCTGGACACGCTCGG
CGCCCCGCCTGCGCTGAGCGAACTGCTGGCGGAAGAGAGTGGTTTGCTGTTGGTCACCGGAGCGACGGGCAGCGGCAAGT
CGACCACCCTGGCGGCAATGGTGGGGCACCTCAATCAGCATCTTGATGGCCATATTCTGACGCTTGAAGATCCGGTGGAA
TTTATTCATCACAGCGAGCGCTGTCTGATCCAGCAGCGGGAAGTTGGCCGTCACTGTCCTTCGTTCGCCGCGGCGCTCCG
CGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGCTCGGGGAGCTACGCGATAGCGAAACCATTCGCCTGGCGTTAACCG
CGGCGGAGACCGGGCACCTGGTAATGGCGACATTACATACCCGCGGCGCGGCGCCGGCGGTGGAGAGATTGATCGATGTC
TTTCCGGCGGAGGAGAAAGATCAGGTTCGTAGCCAGCTTGCCGGAAGCCTGTGCGCGGTGCTGGCGCAAAAATTGTTACC
TGCACGTCAGGGCGGGAGAGTTGCGCTATACGAGCTTCTGGTCAACACGCCAGCGGTGGCGAATTTGATCCGTGAAGGAA
AAGTACATCAATTGCCTGGTGTGATGCAGACCGGGATGCAGGCCGGAATGTTGACCTTTACCCAGAGTTTTCAGCAACGT
GTCGCTGCAGGCGCGCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.368

100

0.518
  pilT Vibrio cholerae strain A1552

51.368

100

0.518
  pilT Acinetobacter baylyi ADP1

48.936

100

0.494
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Acinetobacter baumannii D1279779

48.024

100

0.485
  pilT Acinetobacter baumannii strain A118

48.024

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter nosocomialis M2

47.72

100

0.482
  pilT Legionella pneumophila strain ERS1305867

48.148

99.387

0.479
  pilT Legionella pneumophila strain Lp02

48.148

99.387

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.795

97.239

0.436
  pilU Vibrio cholerae strain A1552

41.358

99.387

0.411
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

39.875

98.466

0.393