Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M5S62_RS19315 Genome accession   NZ_CP103611
Coordinates   4058919..4059899 (-) Length   326 a.a.
NCBI ID   WP_259387450.1    Uniprot ID   -
Organism   Enterobacter cloacae strain 3143     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4053919..4064899
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5S62_RS19285 (M5S62_19285) - 4054795..4055292 (+) 498 WP_028027997.1 SprT family zinc-dependent metalloprotease -
  M5S62_RS19290 (M5S62_19290) endA 4055387..4056094 (+) 708 WP_259387449.1 deoxyribonuclease I -
  M5S62_RS19295 (M5S62_19295) rsmE 4056147..4056878 (+) 732 WP_013098662.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M5S62_RS19300 (M5S62_19300) gshB 4056898..4057839 (+) 942 WP_129328221.1 glutathione synthase -
  M5S62_RS19305 (M5S62_19305) - 4057932..4058492 (+) 561 WP_129253130.1 YqgE/AlgH family protein -
  M5S62_RS19310 (M5S62_19310) ruvX 4058492..4058908 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  M5S62_RS19315 (M5S62_19315) pilT 4058919..4059899 (-) 981 WP_259387450.1 type IV pilus twitching motility protein PilT Machinery gene
  M5S62_RS19320 (M5S62_19320) - 4059917..4060618 (+) 702 WP_020687058.1 YggS family pyridoxal phosphate-dependent enzyme -
  M5S62_RS19325 (M5S62_19325) - 4060640..4061206 (+) 567 WP_013098667.1 YggT family protein -
  M5S62_RS19330 (M5S62_19330) yggU 4061203..4061490 (+) 288 WP_259387451.1 DUF167 family protein YggU -
  M5S62_RS19335 (M5S62_19335) - 4061503..4062096 (+) 594 WP_058680986.1 XTP/dITP diphosphatase -
  M5S62_RS19340 (M5S62_19340) hemW 4062089..4063237 (+) 1149 WP_130626698.1 radical SAM family heme chaperone HemW -
  M5S62_RS19345 (M5S62_19345) - 4063294..4063632 (+) 339 WP_058678487.1 endonuclease domain-containing protein -
  M5S62_RS19350 (M5S62_19350) - 4063715..4064431 (-) 717 WP_014833228.1 DUF2884 domain-containing protein -
  M5S62_RS19355 (M5S62_19355) - 4064488..4064814 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35711.77 Da        Isoelectric Point: 6.4995

>NTDB_id=723683 M5S62_RS19315 WP_259387450.1 4058919..4059899(-) (pilT) [Enterobacter cloacae strain 3143]
MDVEEIVALSVKHNVSNLHLCSDSPPRWRRVGRLEPAPFPFPDVDALLKTWLNDEQQGAWWASGQVDFAVTLTGNQRLRA
SAFKQMKGNSITLRLLPRACPQLSALGVPRAIPELLSHDSGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FVYQSERCLIQQREIGLHSPSFAEALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=723683 M5S62_RS19315 WP_259387450.1 4058919..4059899(-) (pilT) [Enterobacter cloacae strain 3143]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCCAATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGAGTAGGCCGCCTTGAACCGGCTCCCTTTCCCTTTCCCGATGTGGATGCGTTACTGAAAACGTGGCTCAACG
ATGAGCAGCAGGGGGCATGGTGGGCAAGCGGGCAGGTGGATTTTGCCGTGACCCTTACGGGAAACCAACGCCTGCGCGCA
AGCGCCTTTAAACAGATGAAGGGCAACTCAATCACGCTACGCCTGTTGCCCCGGGCCTGTCCGCAGCTCTCCGCCCTGGG
GGTTCCCCGGGCTATCCCGGAACTCTTATCCCACGACAGCGGGCTGATACTGGTGACGGGGGCAACCGGCAGCGGCAAAT
CTACCACCCTTGCGGCAATGGTGGATTTTCTTAATCACCAGACGGATGGGCATATTCTGACGCTGGAAGATCCGGTGGAG
TTTGTCTATCAGAGTGAACGTTGCCTGATTCAGCAGCGGGAGATCGGCCTGCACAGTCCCTCATTTGCCGAGGCGCTGCG
CGCTGCGTTGCGTGAAGATCCGGATGTCATCCTGCTGGGAGAACTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CCGCTGAAACCGGGCATCTGGTGCTGGCGACGCTGCACACGCGCGGTGCCTCTCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGACCCGGTGCGTAATCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAGACTGCTTCC
CGACCTGCAGGGTGGTCGCGTCGCGCTGTATGAGCTGCTGGTGAATACGCCAGCGGCAGCGAACCTTATCCGCGAAGGTA
AAACGTGGCAGTTGCCCGGGATTATTCAAACCGGTCAGCAGGCGGGGATGCAGAACTTTGACCAGAGCCTGGCAGAGCGA
CGGGCGCAGGGGCGGTTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(72-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Acinetobacter baumannii strain A118

47.706

100

0.479
  pilT Acinetobacter baumannii D1279779

47.706

100

0.479
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Acinetobacter nosocomialis M2

48.125

98.16

0.472
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

98.773

0.423
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilB Haemophilus influenzae 86-028NP

33.427

100

0.365