Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M5T18_RS04180 Genome accession   NZ_CP103559
Coordinates   863662..864642 (+) Length   326 a.a.
NCBI ID   WP_001389259.1    Uniprot ID   -
Organism   Escherichia coli strain 3059     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 858662..869642
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5T18_RS04150 (M5T18_04150) yggM 859177..860184 (+) 1008 WP_000745217.1 DUF1202 family protein -
  M5T18_RS04155 (M5T18_04155) hemW 860339..861475 (-) 1137 WP_000239928.1 radical SAM family heme chaperone HemW -
  M5T18_RS04160 (M5T18_04160) rdgB 861468..862061 (-) 594 WP_001174735.1 XTP/dITP diphosphatase -
  M5T18_RS04165 (M5T18_04165) yggU 862069..862359 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  M5T18_RS04170 (M5T18_04170) yggT 862356..862922 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  M5T18_RS04175 (M5T18_04175) yggS 862940..863644 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  M5T18_RS04180 (M5T18_04180) pilT 863662..864642 (+) 981 WP_001389259.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M5T18_RS04185 (M5T18_04185) ruvX 864817..865233 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  M5T18_RS04190 (M5T18_04190) yqgE 865233..865796 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  M5T18_RS04195 (M5T18_04195) gshB 865905..866855 (-) 951 WP_000593273.1 glutathione synthase -
  M5T18_RS04200 (M5T18_04200) rsmE 866868..867599 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M5T18_RS04205 (M5T18_04205) endA 867679..868386 (-) 708 WP_000286517.1 deoxyribonuclease I -
  M5T18_RS04210 (M5T18_04210) yggI 868481..868978 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35984.20 Da        Isoelectric Point: 5.7980

>NTDB_id=723370 M5T18_RS04180 WP_001389259.1 863662..864642(+) (pilT) [Escherichia coli strain 3059]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAVQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=723370 M5T18_RS04180 WP_001389259.1 863662..864642(+) (pilT) [Escherichia coli strain 3059]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCATTCGCGCAACGGCAAGGCATTTCGCTGGCGTTACGGCTGTTGCCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGAAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAAATCGGTTTGCATTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGCGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGACACCTGGTACTGGCAACCTTACATACACGTGGTGCCGTGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCAGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTTTCAGCAGCGA
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365