Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M5R35_RS03895 Genome accession   NZ_CP103554
Coordinates   803210..804190 (+) Length   326 a.a.
NCBI ID   WP_014226838.1    Uniprot ID   A0A0H3H7U5
Organism   Klebsiella michiganensis strain 5088     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 798210..809190
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5R35_RS03865 (M5R35_03865) - 798592..799578 (-) 987 WP_014226841.1 TRAP transporter substrate-binding protein -
  M5R35_RS03870 (M5R35_03870) hemW 799883..801019 (-) 1137 WP_014226840.1 radical SAM family heme chaperone HemW -
  M5R35_RS03875 (M5R35_03875) - 801012..801605 (-) 594 WP_004854157.1 XTP/dITP diphosphatase -
  M5R35_RS03880 (M5R35_03880) yggU 801618..801908 (-) 291 WP_004854155.1 DUF167 family protein YggU -
  M5R35_RS03885 (M5R35_03885) - 801905..802471 (-) 567 WP_004105714.1 YggT family protein -
  M5R35_RS03890 (M5R35_03890) - 802489..803193 (-) 705 WP_014226839.1 YggS family pyridoxal phosphate-dependent enzyme -
  M5R35_RS03895 (M5R35_03895) pilT 803210..804190 (+) 981 WP_014226838.1 type IV pilus twitching motility protein PilT Machinery gene
  M5R35_RS03900 (M5R35_03900) - 804539..805192 (+) 654 WP_014226837.1 LuxR C-terminal-related transcriptional regulator -
  M5R35_RS03905 (M5R35_03905) ruvX 805248..805664 (-) 417 WP_014226836.1 Holliday junction resolvase RuvX -
  M5R35_RS03910 (M5R35_03910) - 805664..806227 (-) 564 WP_014226835.1 YqgE/AlgH family protein -
  M5R35_RS03915 (M5R35_03915) gshB 806404..807351 (-) 948 WP_014226834.1 glutathione synthase -
  M5R35_RS03920 (M5R35_03920) rsmE 807372..808103 (-) 732 WP_014226833.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M5R35_RS03925 (M5R35_03925) endA 808195..808902 (-) 708 WP_014226832.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35191.38 Da        Isoelectric Point: 6.6525

>NTDB_id=723323 M5R35_RS03895 WP_014226838.1 803210..804190(+) (pilT) [Klebsiella michiganensis strain 5088]
MELEEIVALSVKHNVSDLHLCNASTPRWRRQGKLEPAPFTSPDIGKILADWLNAEQLAQWQACGQVDFALAPAGGPRLRA
SAFVHNRGLSLALRLLAETCPQLADLGAPAALTELLNEESGLILVTGATGSGKSTTLAAMVGYLNQRLNGHILTLEDPVE
FIHHSERCLIQQREIGRHCPSFAAALRAALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAQAVERLVDV
FPAQEKEQVRSQLAGSLCAVLAQKLFPDTAGGRVALYELLVNTPAVANLIREGKTHQLPGVMQTGQQFGMQTFTQSFQQR
TAAGLL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=723323 M5R35_RS03895 WP_014226838.1 803210..804190(+) (pilT) [Klebsiella michiganensis strain 5088]
ATGGAACTGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAATGCTTCAACGCCGCG
CTGGCGTCGCCAGGGCAAGCTGGAGCCTGCGCCTTTTACCTCTCCGGATATTGGCAAGATACTCGCTGATTGGCTCAACG
CCGAGCAGCTCGCCCAGTGGCAGGCCTGCGGGCAGGTTGATTTCGCGCTGGCTCCGGCCGGCGGCCCGCGCCTGCGCGCC
AGCGCATTTGTCCACAACCGCGGCCTGTCGCTGGCCCTGCGTCTGCTGGCAGAAACCTGCCCGCAGCTGGCGGATCTCGG
CGCGCCGGCCGCCTTAACGGAGCTGCTCAATGAGGAGAGCGGGCTTATCCTGGTCACGGGCGCCACCGGTAGCGGGAAAT
CCACCACCCTGGCGGCGATGGTGGGCTATCTTAATCAGCGCCTCAATGGCCATATCCTGACGCTGGAAGATCCGGTTGAG
TTTATTCATCACAGCGAGCGCTGCCTGATCCAGCAGCGGGAAATCGGTCGCCACTGCCCCTCATTTGCCGCGGCCCTGCG
CGCTGCGCTGCGTCAGGATCCGGATGTCATTCTGCTAGGGGAACTGCGTGACAGCGAAACGATCCGCCTGGCATTGACCG
CGGCGGAGACCGGACATCTGGTTATGGCGACGCTGCATACGCGCGGTGCGGCACAGGCGGTTGAAAGGCTGGTGGATGTT
TTTCCCGCTCAGGAGAAAGAGCAGGTTCGTAGCCAGCTGGCGGGAAGTTTATGCGCGGTGCTGGCACAAAAATTGTTTCC
GGATACTGCTGGCGGAAGAGTTGCGCTATACGAGCTTTTGGTTAATACCCCGGCGGTGGCGAATTTGATCCGCGAGGGGA
AAACGCATCAGCTGCCCGGCGTGATGCAAACCGGGCAGCAATTCGGGATGCAAACTTTTACGCAAAGCTTTCAGCAACGT
ACGGCTGCGGGTCTTTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3H7U5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.848

100

0.503
  pilT Vibrio cholerae strain A1552

49.848

100

0.503
  pilT Neisseria meningitidis 8013

48.182

100

0.488
  pilT Neisseria gonorrhoeae MS11

47.879

100

0.485
  pilT Legionella pneumophila strain Lp02

48.113

97.546

0.469
  pilT Legionella pneumophila strain ERS1305867

48.113

97.546

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.201

100

0.466
  pilT Acinetobacter baumannii strain A118

45.455

100

0.46
  pilT Acinetobacter baumannii D1279779

45.455

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.593

100

0.46
  pilT Acinetobacter nosocomialis M2

45.152

100

0.457
  pilT Acinetobacter baylyi ADP1

44.985

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.479

97.239

0.433
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.414
  pilU Vibrio cholerae strain A1552

40.683

98.773

0.402
  pilU Acinetobacter baylyi ADP1

38.39

99.08

0.38