Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M5T26_RS03685 Genome accession   NZ_CP103487
Coordinates   743081..744061 (+) Length   326 a.a.
NCBI ID   WP_023290994.1    Uniprot ID   -
Organism   Klebsiella quasipneumoniae subsp. quasipneumoniae strain 5463     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 738081..749061
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5T26_RS03655 (M5T26_03655) - 738463..739449 (-) 987 WP_107112155.1 TRAP transporter substrate-binding protein -
  M5T26_RS03660 (M5T26_03660) hemW 739754..740890 (-) 1137 WP_107112156.1 radical SAM family heme chaperone HemW -
  M5T26_RS03665 (M5T26_03665) - 740883..741476 (-) 594 WP_023290995.1 XTP/dITP diphosphatase -
  M5T26_RS03670 (M5T26_03670) yggU 741489..741779 (-) 291 WP_004205183.1 DUF167 family protein YggU -
  M5T26_RS03675 (M5T26_03675) - 741776..742342 (-) 567 WP_072421873.1 YggT family protein -
  M5T26_RS03680 (M5T26_03680) - 742363..743064 (-) 702 WP_004205181.1 YggS family pyridoxal phosphate-dependent enzyme -
  M5T26_RS03685 (M5T26_03685) pilT 743081..744061 (+) 981 WP_023290994.1 type IV pilus twitching motility protein PilT Machinery gene
  M5T26_RS03690 (M5T26_03690) - 744410..745063 (+) 654 WP_032454048.1 LuxR C-terminal-related transcriptional regulator -
  M5T26_RS03695 (M5T26_03695) ruvX 745115..745531 (-) 417 WP_072421871.1 Holliday junction resolvase RuvX -
  M5T26_RS03700 (M5T26_03700) - 745531..746094 (-) 564 WP_002916603.1 YqgE/AlgH family protein -
  M5T26_RS03705 (M5T26_03705) gshB 746269..747219 (-) 951 WP_072421870.1 glutathione synthase -
  M5T26_RS03710 (M5T26_03710) rsmE 747240..747971 (-) 732 WP_214632850.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M5T26_RS03715 (M5T26_03715) endA 748063..748770 (-) 708 WP_032454045.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35413.82 Da        Isoelectric Point: 6.8575

>NTDB_id=723007 M5T26_RS03685 WP_023290994.1 743081..744061(+) (pilT) [Klebsiella quasipneumoniae subsp. quasipneumoniae strain 5463]
MKLEEIVALSVKHNVSDLHLCNSAAPRWRRQGKLESAPFPAPDIADLLNDWLDAAQFQHWQEHGQIDFALTLACGSRLRA
SAFAHTRGISLVLRLLPVQCPRLETLGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVGHLNQHLDGHILTLEDPVE
FIHHSERCLIQQREIGRHCPSFSAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVERLIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPAVANLIREGKVHQLPGVMQTGMQAGMLTFTQSFQQR
VATGQL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=723007 M5T26_RS03685 WP_023290994.1 743081..744061(+) (pilT) [Klebsiella quasipneumoniae subsp. quasipneumoniae strain 5463]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATTCCGCCGCACCGCG
CTGGCGGCGGCAGGGCAAGCTGGAGTCCGCTCCCTTTCCCGCGCCGGATATCGCGGATTTACTCAACGACTGGCTCGATG
CCGCACAGTTCCAGCACTGGCAGGAGCACGGCCAGATTGACTTTGCGCTGACTCTGGCCTGCGGTTCGCGGCTACGCGCC
AGCGCTTTCGCCCATACGCGCGGAATATCGCTGGTGCTGCGTCTGCTGCCCGTCCAGTGCCCGCGTCTGGAGACGCTGGG
CGCGCCGCCTGCGCTAAGCGAGCTGCTGGCGGAAGAGAGTGGCCTGCTGCTGGTCACCGGGGCGACGGGCAGCGGCAAGT
CGACTACCCTGGCGGCGATGGTCGGGCATCTCAACCAGCATCTTGATGGCCATATTCTGACGCTGGAGGATCCGGTGGAG
TTTATTCATCACAGCGAGCGCTGCCTGATCCAGCAGCGGGAGATTGGCCGTCACTGTCCTTCGTTCTCCGCGGCGCTTCG
GGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGCTTGGGGAGTTACGCGACAGCGAAACCATCCGTCTGGCGTTGACGG
CTGCGGAAACCGGGCATCTGGTGATGGCGACATTACACACCCGCGGCGCGGCGCCGGCGGTGGAGAGATTGATCGATGTC
TTTCCGGCAGAGGAGAAAGATCAGGTTCGGAGTCAGCTGGCCGGAAGCCTGTGCGCGGTGCTGGCGCAAAAATTATTACC
CGCACGTCAGGGCGGCAGAGTTGCGCTATACGAGCTTCTGGTCAATACGCCGGCGGTGGCGAATTTGATCCGCGAAGGAA
AAGTGCACCAGCTGCCCGGTGTAATGCAGACCGGGATGCAGGCCGGAATGCTGACATTTACCCAGAGTTTTCAGCAACGT
GTGGCCACAGGCCAGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.682

100

0.518
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.682

100

0.518
  pilT Acinetobacter baylyi ADP1

48.624

100

0.488
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Acinetobacter nosocomialis M2

47.706

100

0.479
  pilT Legionella pneumophila strain ERS1305867

47.84

99.387

0.475
  pilT Legionella pneumophila strain Lp02

47.84

99.387

0.475
  pilT Pseudomonas stutzeri DSM 10701

47.401

100

0.475
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.164

97.239

0.429
  pilU Vibrio cholerae strain A1552

40.426

100

0.408
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

39.252

98.466

0.386