Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M5T42_RS04415 Genome accession   NZ_CP103465
Coordinates   895762..896742 (+) Length   326 a.a.
NCBI ID   WP_001546096.1    Uniprot ID   A0A7I0KYK3
Organism   Escherichia coli O25b:H4-ST131 strain 5392     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 890762..901742
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5T42_RS04385 (M5T42_04385) yggM 891363..892370 (+) 1008 WP_000745201.1 DUF1202 family protein -
  M5T42_RS04390 (M5T42_04390) hemW 892439..893575 (-) 1137 WP_000239978.1 radical SAM family heme chaperone HemW -
  M5T42_RS04395 (M5T42_04395) rdgB 893568..894161 (-) 594 WP_001174747.1 XTP/dITP diphosphatase -
  M5T42_RS04400 (M5T42_04400) yggU 894169..894459 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  M5T42_RS04405 (M5T42_04405) yggT 894456..895022 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  M5T42_RS04410 (M5T42_04410) yggS 895040..895744 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  M5T42_RS04415 (M5T42_04415) pilT 895762..896742 (+) 981 WP_001546096.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M5T42_RS04420 (M5T42_04420) ruvX 896921..897337 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  M5T42_RS04425 (M5T42_04425) yqgE 897337..897900 (-) 564 WP_001355636.1 YqgE/AlgH family protein -
  M5T42_RS04430 (M5T42_04430) gshB 898009..898959 (-) 951 WP_000593260.1 glutathione synthase -
  M5T42_RS04435 (M5T42_04435) rsmE 898972..899703 (-) 732 WP_001531929.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M5T42_RS04440 (M5T42_04440) endA 899783..900490 (-) 708 WP_001305312.1 deoxyribonuclease I -
  M5T42_RS04445 (M5T42_04445) yggI 900585..901082 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35944.14 Da        Isoelectric Point: 5.7980

>NTDB_id=722941 M5T42_RS04415 WP_001546096.1 895762..896742(+) (pilT) [Escherichia coli O25b:H4-ST131 strain 5392]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLVNTPAVGNLIREGKTHQLLHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=722941 M5T42_RS04415 WP_001546096.1 895762..896742(+) (pilT) [Escherichia coli O25b:H4-ST131 strain 5392]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCCGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CAGCAGAAACCGGACACCTGGTGCTGGCAACTTTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGGCTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGGTTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACTGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGACGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7I0KYK3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilB Legionella pneumophila strain ERS1305867

31.152

100

0.365