Detailed information NTG0722777

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: NYO12_RS03780
• Genome accession: NZ_CP103420
• Coordinates: 749042..750022 (+)
• Length: 326 a.a.
• NCBI ID: WP_042948086.1
• Uniprot ID: A0A2V3KQC4
• Organism: Klebsiella variicola strain KV1
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IScluster/Tn	750129..750823	749042..750022	flank	107

Gene organization within MGE regions

Location: 749042..750823

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NYO12_RS03780 (NYO12_03780)	pilT	749042..750022 (+)	981	WP_042948086.1	type IV pilus twitching motility protein PilT	Machinery gene

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35424.04 Da
• Isoelectric Point: 6.7658

>NTDB_id=722777 NYO12_RS03780 WP_042948086.1 749042..750022(+) (pilT) [Klebsiella variicola strain KV1]
MKLEEIVALSVKHNVSDLHLCNSAAPRWRRQGKLEPAPFPAPDIPDLLNCWLDAAQLQHWQEQGQIDFALTLACGLRLRA
SAFAHTRGISLVLRLLPEQCPRLETLGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVGHLNQRLDGHILTLEDPVE
FIHHSERCLILQREIGRHCPSFAAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVERLIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPAVANLIREGKVHQLPGIMQTGMQAGMLTFTQSFQQR
VAAGEL

Nucleotide

• Length: 981 bp

>NTDB_id=722777 NYO12_RS03780 WP_042948086.1 749042..750022(+) (pilT) [Klebsiella variicola strain KV1]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATTCCGCCGCACCGCG
CTGGCGGCGGCAGGGCAAGCTGGAGCCCGCCCCGTTTCCCGCGCCGGATATCCCGGATTTACTCAACTGCTGGCTTGATG
CCGCACAGTTACAGCATTGGCAGGAACAGGGCCAGATTGACTTTGCGCTGACCCTGGCCTGCGGTTTGCGGCTGCGCGCC
AGCGCTTTCGCCCATACGCGTGGAATATCGCTGGTGCTACGCCTTCTGCCTGAACAGTGCCCGCGTCTGGAGACGCTGGG
CGCCCCGCCTGCGCTGAGCGAGCTGCTGGCGGAAGAGAGTGGCCTGCTGCTGGTCACCGGAGCGACGGGCAGCGGCAAGT
CGACCACCCTGGCGGCGATGGTGGGGCATCTCAACCAGCGTCTTGATGGCCATATTCTGACCCTGGAGGACCCGGTGGAG
TTTATTCATCACAGCGAGCGCTGCCTGATCCTGCAGCGGGAGATTGGCCGTCATTGTCCTTCGTTCGCCGCGGCGCTCCG
CGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGCTCGGGGAGCTACGCGACAGCGAAACCATTCGTTTGGCGTTAACGG
CTGCGGAGACCGGACATCTGGTAATGGCGACATTACATACCCGCGGCGCGGCGCCGGCGGTGGAGAGATTGATCGATGTC
TTTCCGGCAGAGGAGAAAGATCAGGTTCGTAGTCAGCTGGCCGGAAGCCTGTGCGCGGTGCTGGCGCAAAAATTGTTGCC
TGCGCGTCAGGGCGGCAGAGTTGCGCTATACGAGCTTTTGGTCAATACCCCCGCAGTAGCTAACTTGATCCGTGAAGGAA
AAGTGCACCAGTTGCCCGGCATAATGCAGACCGGGATGCAGGCCGGAATGCTGACATTTACGCAGAGTTTTCAGCAACGT
GTCGCCGCAGGCGAGCTGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (11-266)

3D structure

• AlphaFold DB: A0A2V3KQC4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	51.682	100	0.518
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	51.682	100	0.518
pilT	Acinetobacter baylyi ADP1	48.012	100	0.482
pilT	Neisseria meningitidis 8013	47.561	100	0.479
pilT	Acinetobacter baumannii strain A118	47.706	100	0.479
pilT	Acinetobacter baumannii D1279779	47.706	100	0.479
pilT	Acinetobacter nosocomialis M2	47.401	100	0.475
pilT	Neisseria gonorrhoeae MS11	47.256	100	0.475
pilT	Legionella pneumophila strain ERS1305867	47.531	99.387	0.472
pilT	Legionella pneumophila strain Lp02	47.531	99.387	0.472
pilT	Pseudomonas stutzeri DSM 10701	46.789	100	0.469
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.849	97.239	0.426
pilU	Vibrio cholerae strain A1552	40.122	100	0.405
pilU	Pseudomonas stutzeri DSM 10701	40	100	0.405
pilU	Acinetobacter baylyi ADP1	38.941	98.466	0.383