Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NYO13_RS03670 Genome accession   NZ_CP103419
Coordinates   747034..748014 (+) Length   326 a.a.
NCBI ID   WP_008806466.1    Uniprot ID   -
Organism   Klebsiella variicola strain KV4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 742034..753014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYO13_RS03635 (NYO13_03635) - 742570..742896 (+) 327 WP_012967224.1 YggL family protein -
  NYO13_RS03640 (NYO13_03640) - 742947..743666 (+) 720 WP_048330664.1 DUF2884 domain-containing protein -
  NYO13_RS03645 (NYO13_03645) hemW 743707..744843 (-) 1137 WP_008806470.1 radical SAM family heme chaperone HemW -
  NYO13_RS03650 (NYO13_03650) - 744836..745429 (-) 594 WP_008806469.1 XTP/dITP diphosphatase -
  NYO13_RS03655 (NYO13_03655) yggU 745442..745732 (-) 291 WP_008806468.1 DUF167 family protein YggU -
  NYO13_RS03660 (NYO13_03660) - 745729..746295 (-) 567 WP_004205182.1 YggT family protein -
  NYO13_RS03665 (NYO13_03665) - 746316..747017 (-) 702 WP_008806467.1 YggS family pyridoxal phosphate-dependent enzyme -
  NYO13_RS03670 (NYO13_03670) pilT 747034..748014 (+) 981 WP_008806466.1 type IV pilus twitching motility protein PilT Machinery gene
  NYO13_RS03675 (NYO13_03675) - 748363..749016 (+) 654 WP_008806465.1 LuxR C-terminal-related transcriptional regulator -
  NYO13_RS03680 (NYO13_03680) ruvX 749069..749485 (-) 417 WP_012540565.1 Holliday junction resolvase RuvX -
  NYO13_RS03685 (NYO13_03685) - 749485..750048 (-) 564 WP_008806463.1 YqgE/AlgH family protein -
  NYO13_RS03690 (NYO13_03690) gshB 750222..751172 (-) 951 WP_008806462.1 glutathione synthase -
  NYO13_RS03695 (NYO13_03695) rsmE 751193..751924 (-) 732 WP_008806461.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NYO13_RS03700 (NYO13_03700) endA 752016..752723 (-) 708 WP_008806460.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35447.08 Da        Isoelectric Point: 6.8129

>NTDB_id=722754 NYO13_RS03670 WP_008806466.1 747034..748014(+) (pilT) [Klebsiella variicola strain KV4]
MKLEEIVALSVKHNVSDLHLCNSAAPRWRRQGKLEPAPFPAPDIPDLLHCWLDAAQLQHWQEQGQIDFALTLACGLRLRA
SAFAHTRGISLVLRLLPEQCPRLETLGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVGHLNQRLDGHILTLEDPVE
FIHHSERCLILQREIGRHCPSFAAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVERLIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPAVANLIREGKVHQLPGIMQTGMQAGMLTFTQSFQQR
VAAGEL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=722754 NYO13_RS03670 WP_008806466.1 747034..748014(+) (pilT) [Klebsiella variicola strain KV4]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATTCCGCCGCACCGCG
CTGGCGGCGGCAGGGCAAGCTGGAGCCCGCCCCGTTTCCCGCGCCGGATATCCCGGATTTGCTCCACTGCTGGCTTGATG
CCGCACAGTTACAGCATTGGCAGGAACAGGGCCAGATTGACTTTGCGCTGACCCTGGCCTGCGGTTTGCGGCTGCGCGCC
AGCGCTTTCGCCCATACGCGTGGAATATCGCTGGTGCTACGCCTTCTGCCTGAACAGTGCCCGCGTCTGGAGACGCTGGG
CGCCCCGCCTGCGCTGAGCGAGCTGCTGGCGGAAGAGAGTGGTTTGCTGTTGGTCACCGGAGCGACGGGCAGCGGCAAGT
CGACCACCCTGGCGGCGATGGTGGGGCATCTCAACCAGCGTCTTGATGGCCATATTCTGACCCTGGAGGACCCGGTGGAG
TTTATTCATCACAGCGAGCGCTGCCTGATCCTGCAGCGGGAGATTGGCCGTCATTGTCCTTCGTTCGCCGCGGCGCTCCG
CGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGCTCGGGGAGCTACGCGACAGCGAAACCATTCGTCTGGCGTTAACGG
CTGCGGAGACCGGACATCTGGTAATGGCGACATTACATACCCGCGGCGCGGCGCCGGCGGTGGAGAGATTGATCGATGTC
TTTCCGGCAGAGGAGAAAGATCAGGTTCGTAGTCAGCTGGCCGGAAGCCTGTGCGCGGTGCTGGCGCAAAAATTGTTACC
TGCACGTCAGGGCGGGAGAGTTGCGCTATACGAGCTTTTGGTCAATACCCCCGCAGTAGCTAACTTGATCCGTGAAGGAA
AAGTGCATCAGTTGCCCGGCATAATGCAGACCGGGATGCAGGCCGGAATGCTGACATTTACGCAGAGTTTTCAGCAACGT
GTCGCCGCAGGCGAGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.682

100

0.518
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.682

100

0.518
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Acinetobacter baumannii D1279779

47.706

100

0.479
  pilT Acinetobacter baumannii strain A118

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

47.401

100

0.475
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Legionella pneumophila strain ERS1305867

47.531

99.387

0.472
  pilT Legionella pneumophila strain Lp02

47.531

99.387

0.472
  pilT Pseudomonas stutzeri DSM 10701

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.849

97.239

0.426
  pilU Vibrio cholerae strain A1552

40.426

100

0.408
  pilU Pseudomonas stutzeri DSM 10701

40.303

100

0.408
  pilU Acinetobacter baylyi ADP1

38.941

98.466

0.383