Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NX761_RS08530 Genome accession   NZ_CP103306
Coordinates   1784337..1785380 (+) Length   347 a.a.
NCBI ID   WP_046849851.1    Uniprot ID   A0A0F7KG38
Organism   Nitrosomonas sp. PLL12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1779337..1790380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NX761_RS08495 (NX761_08495) - 1779797..1779961 (+) 165 WP_158441447.1 hypothetical protein -
  NX761_RS08500 (NX761_08500) lolD 1780030..1780704 (-) 675 WP_046849846.1 lipoprotein-releasing ABC transporter ATP-binding protein LolD -
  NX761_RS08505 (NX761_08505) - 1780697..1781944 (-) 1248 WP_046849847.1 lipoprotein-releasing ABC transporter permease subunit -
  NX761_RS08510 (NX761_08510) rsmD 1782051..1782671 (+) 621 WP_309567611.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  NX761_RS08515 (NX761_08515) coaD 1782664..1783143 (+) 480 WP_046849848.1 pantetheine-phosphate adenylyltransferase -
  NX761_RS08520 (NX761_08520) - 1783162..1783431 (+) 270 WP_046849849.1 YfhL family 4Fe-4S dicluster ferredoxin -
  NX761_RS08525 (NX761_08525) - 1783400..1784116 (-) 717 WP_046849850.1 YggS family pyridoxal phosphate-dependent enzyme -
  NX761_RS08530 (NX761_08530) pilT 1784337..1785380 (+) 1044 WP_046849851.1 type IV pilus twitching motility protein PilT Machinery gene
  NX761_RS08535 (NX761_08535) pilU 1785516..1786652 (+) 1137 WP_046849852.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NX761_RS08540 (NX761_08540) - 1786794..1787585 (-) 792 WP_046849853.1 carbonic anhydrase -
  NX761_RS08545 (NX761_08545) - 1787631..1787930 (+) 300 WP_158441449.1 hypothetical protein -
  NX761_RS08550 (NX761_08550) - 1788151..1788645 (-) 495 WP_046849855.1 hypothetical protein -
  NX761_RS19670 - 1789082..1790224 (-) 1143 Protein_1717 aldehyde dehydrogenase family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38507.39 Da        Isoelectric Point: 7.1148

>NTDB_id=721840 NX761_RS08530 WP_046849851.1 1784337..1785380(+) (pilT) [Nitrosomonas sp. PLL12]
MNIVELLAFVVKNNASDLHLSAGMPPMIRVHGDVRRINLPAMEHKDVHDMIYDIMNDSQRKFYEEHLECDFSFAVPNLAR
FRVNAFNTHRGAASVMRTIPSKVLTLEQIKAPKIFAEIAQQPRGVVLVTGPTGSGKSTTLAAMIDDINENQYGHILTLED
PIEFVHESKKCLINQREIGKDSHSFANALRAALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLRAVISQALLKTKDGTGRVAAHEIMIGTPAIRNLIREGKVAQMYSTIQTGQSVGMQTLDQN
LADLVKRNVVSMVEARAKAMNKDNFRS

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=721840 NX761_RS08530 WP_046849851.1 1784337..1785380(+) (pilT) [Nitrosomonas sp. PLL12]
ATGAACATAGTAGAACTACTTGCCTTTGTGGTCAAGAATAATGCATCTGATTTGCATTTGTCTGCGGGTATGCCACCAAT
GATACGTGTACATGGTGATGTCCGGCGCATCAATCTGCCAGCCATGGAGCATAAAGATGTCCATGACATGATTTATGACA
TCATGAATGATAGTCAGCGCAAATTCTATGAAGAGCATCTCGAATGCGATTTTTCTTTTGCCGTACCTAATCTTGCGCGT
TTTCGAGTTAATGCATTCAATACTCATCGAGGTGCAGCTTCAGTCATGCGCACAATTCCATCAAAGGTGCTAACACTGGA
GCAGATCAAAGCGCCCAAGATTTTTGCAGAAATTGCGCAGCAACCGCGAGGCGTTGTGCTGGTAACAGGTCCAACCGGTT
CGGGTAAGTCAACTACCCTGGCGGCAATGATCGACGATATTAATGAGAATCAGTATGGTCATATTCTTACACTTGAGGAT
CCGATCGAGTTTGTTCATGAAAGTAAGAAATGCCTGATCAATCAACGTGAAATCGGAAAAGATAGCCATAGTTTCGCTAA
TGCATTGCGTGCAGCTTTACGAGAAGACCCGGACATTATTTTAGTGGGTGAGATGCGAGATCTGGAGACGATTCGTTTGG
CGTTGACAGCGGCAGAAACAGGTCATTTGGTATTCGGTACCCTGCATACGAGTTCTGCCGCCAAAACCATTGATCGTATT
ATCGATGTGTTTCCTGCGGAGGAAAAGGAAATGGTTCGTGCCATGCTATCGGAGTCCCTGCGGGCAGTGATCTCTCAGGC
ATTGTTGAAAACTAAAGATGGGACTGGCCGAGTGGCAGCGCATGAGATCATGATCGGTACCCCCGCCATCCGTAACCTGA
TCCGGGAAGGAAAAGTGGCTCAGATGTATTCAACTATTCAGACCGGTCAGAGTGTAGGAATGCAAACACTCGATCAAAAC
CTGGCTGATCTGGTGAAGCGTAATGTCGTTTCCATGGTGGAGGCAAGAGCCAAAGCTATGAACAAGGACAATTTTCGAAG
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F7KG38

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

76.812

99.424

0.764
  pilT Acinetobacter baumannii D1279779

76.812

99.424

0.764
  pilT Acinetobacter baumannii strain A118

76.812

99.424

0.764
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Pseudomonas stutzeri DSM 10701

73.623

99.424

0.732
  pilT Pseudomonas aeruginosa PAK

73.333

99.424

0.729
  pilT Legionella pneumophila strain Lp02

71.884

99.424

0.715
  pilT Legionella pneumophila strain ERS1305867

71.884

99.424

0.715
  pilT Neisseria gonorrhoeae MS11

68.986

99.424

0.686
  pilT Neisseria meningitidis 8013

68.986

99.424

0.686
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.343

97.406

0.666
  pilT Vibrio cholerae strain A1552

68.343

97.406

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Acinetobacter baylyi ADP1

39.828

100

0.401
  pilU Vibrio cholerae strain A1552

40.173

99.712

0.401
  pilU Pseudomonas stutzeri DSM 10701

39.884

99.712

0.398