Detailed information NTG0721812

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: NX720_RS08565
• Genome accession: NZ_CP103300
• Coordinates: 2253085..2254119 (-)
• Length: 344 a.a.
• NCBI ID: WP_262600709.1
• Uniprot ID: -
• Organism: Endozoicomonas euniceicola strain EF212
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	2252586..2253014	2253085..2254119	flank	71

Gene organization within MGE regions

Location: 2252586..2254119

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NX720_RS08560 (NX720_08560)	tnpA	2252586..2253014 (+)	429	WP_262595354.1	IS200/IS605 family transposase	-
NX720_RS08565 (NX720_08565)	pilT	2253085..2254119 (-)	1035	WP_262600709.1	type IV pilus twitching motility protein PilT	Machinery gene

Sequence

Protein

• Length: 344 a.a.
• Molecular weight: 38108.69 Da
• Isoelectric Point: 6.6622

>NTDB_id=721812 NX720_RS08565 WP_262600709.1 2253085..2254119(-) (pilT) [Endozoicomonas euniceicola strain EF212]
MDITELLAFSFKQGASDLHLSAGLPPMIRMDGDVRRINLPPMDHKQVHGLIYDIMNDKQRRDFEECMQTDFSFEVPGVAR
FRVNAFNQSRGAGAVFRTIPSKVLSMDDLGMGQVFKDISSMPRGLVLVTGPTGSGKSTTLAAMLDYINDTRYDHILTIED
PIEFVHNSKKCLVNQREVHRDTRSFSDALRAALREDPDIILVGEMRDLETIRLALTAAETGHMVFGTLHTTSAAKTVDRI
IDVFPAEEKSMVRSMLSESLQAVVSQTLLKKNGGGRVAAHEIMIGTSAIRNLIREDKVAQMYSAIQTGSSIGMQTLDQCL
KKLVNNSLISHDTAREKAKDPDSL

Nucleotide

• Length: 1035 bp

>NTDB_id=721812 NX720_RS08565 WP_262600709.1 2253085..2254119(-) (pilT) [Endozoicomonas euniceicola strain EF212]
ATGGATATCACCGAGCTGCTGGCATTCAGCTTCAAGCAGGGCGCATCGGATTTACACCTCTCTGCGGGCTTGCCACCCAT
GATTCGTATGGACGGCGATGTACGCCGAATCAACCTGCCTCCCATGGATCATAAACAGGTACACGGCCTGATTTATGACA
TTATGAACGATAAACAGCGACGTGATTTCGAAGAATGCATGCAGACGGACTTTTCTTTTGAAGTACCGGGTGTCGCACGT
TTTCGGGTGAATGCGTTTAACCAGTCCCGTGGTGCGGGGGCGGTGTTTCGTACCATCCCCAGCAAGGTTCTGTCGATGGA
TGACCTGGGTATGGGGCAGGTGTTCAAGGATATCTCTTCCATGCCAAGGGGGCTGGTGCTGGTCACAGGACCTACCGGTT
CCGGTAAGTCAACGACACTGGCCGCCATGCTCGATTACATTAACGATACCCGCTATGACCATATCCTGACCATTGAAGAC
CCCATTGAATTTGTTCATAACTCGAAAAAGTGCCTGGTTAACCAGAGGGAAGTTCATCGGGATACCCGCAGTTTTAGCGA
TGCCCTGCGGGCAGCCCTGCGTGAAGACCCGGACATTATTCTGGTGGGTGAAATGCGAGACCTTGAAACCATTCGCCTGG
CTCTGACAGCCGCTGAAACCGGCCACATGGTGTTTGGTACTCTACATACGACGTCTGCCGCCAAAACCGTTGACCGGATT
ATTGATGTGTTTCCCGCTGAAGAAAAATCAATGGTGCGCTCCATGCTGTCCGAGTCTTTGCAGGCAGTGGTTTCCCAGAC
TCTGCTTAAGAAAAACGGCGGTGGGCGTGTAGCTGCCCATGAAATCATGATAGGCACTTCTGCCATTCGTAACCTGATTC
GTGAAGACAAAGTGGCACAGATGTATTCGGCGATACAGACAGGCAGTTCCATCGGTATGCAGACGCTCGACCAGTGCCTG
AAAAAACTGGTCAATAACAGTTTGATTTCACACGACACTGCCCGGGAAAAAGCCAAGGATCCGGATAGCCTTTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (11-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Pseudomonas stutzeri DSM 10701	85.465	100	0.855
pilT	Pseudomonas aeruginosa PAK	85.174	100	0.852
pilT	Acinetobacter nosocomialis M2	82.267	100	0.823
pilT	Acinetobacter baumannii D1279779	82.267	100	0.823
pilT	Acinetobacter baumannii strain A118	82.267	100	0.823
pilT	Acinetobacter baylyi ADP1	79.36	100	0.794
pilT	Legionella pneumophila strain Lp02	76.036	98.256	0.747
pilT	Legionella pneumophila strain ERS1305867	76.036	98.256	0.747
pilT	Neisseria meningitidis 8013	65.217	100	0.654
pilT	Neisseria gonorrhoeae MS11	64.928	100	0.651
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	65.875	97.965	0.645
pilT	Vibrio cholerae strain A1552	65.875	97.965	0.645
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	52.924	99.419	0.526
pilU	Pseudomonas stutzeri DSM 10701	40.896	97.384	0.398
pilU	Acinetobacter baylyi ADP1	39.017	100	0.392
pilU	Vibrio cholerae strain A1552	39.13	100	0.392