Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TEPE_RS01070 Genome accession   NZ_CP102984
Coordinates   165896..166957 (+) Length   353 a.a.
NCBI ID   WP_228108453.1    Uniprot ID   -
Organism   Terrisporobacter petrolearius strain JCM 19845     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 160896..171957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TEPE_RS01050 spoVG 161008..161286 (+) 279 WP_018591966.1 septation regulator SpoVG -
  TEPE_RS01055 tyrS 161869..163092 (+) 1224 WP_228108469.1 tyrosine--tRNA ligase -
  TEPE_RS01060 glmU 163305..164681 (+) 1377 WP_228108454.1 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU -
  TEPE_RS01065 - 164742..165692 (+) 951 WP_018591963.1 ribose-phosphate diphosphokinase -
  TEPE_RS01070 pilT 165896..166957 (+) 1062 WP_228108453.1 type IV pilus twitching motility protein PilT Machinery gene
  TEPE_RS01075 - 167233..167796 (+) 564 WP_228108452.1 prepilin peptidase -
  TEPE_RS01080 pth 167809..168372 (+) 564 WP_228108451.1 aminoacyl-tRNA hydrolase -
  TEPE_RS01085 mfd 168386..171778 (+) 3393 WP_228108450.1 transcription-repair coupling factor -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 39538.38 Da        Isoelectric Point: 7.8094

>NTDB_id=721065 TEPE_RS01070 WP_228108453.1 165896..166957(+) (pilT) [Terrisporobacter petrolearius strain JCM 19845]
MNIFDILKIAIELEASDIHITVGSMPVARAKGTFTKLTQNILTKHDTEKMTKEIAGDKNFKKIEKYGECDFSVAVESGER
FRVNAYKQKGSYAIAIRAITSQIPSFDDLGLPDILKNFTEKHKGLVLVTGPTGSGKSTTLASLIDIINKNQQRHIITLED
PIEYVHQHKKSLVNQREIGQDTESFNSALKAILRQDPDVILVGEMRDLETISIALTAAETGHLVFSTLHTIGAAKTIDRI
VDMFPSEQQQQVRTQLSTVCEGVISQQLLQTIDGKNRVVAMEVMVSNPAIRNLIRENKTYQIQNIIQTGSKYGMQTMDQE
LVNLYMKGKISKHSALSRCTDYEYTCRLVGDRY

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=721065 TEPE_RS01070 WP_228108453.1 165896..166957(+) (pilT) [Terrisporobacter petrolearius strain JCM 19845]
ATGAATATATTTGATATATTAAAAATAGCAATAGAACTTGAGGCATCAGATATACATATTACAGTAGGCAGTATGCCAGT
TGCTAGAGCAAAAGGAACATTTACAAAATTAACACAAAATATACTAACAAAGCATGATACAGAAAAGATGACAAAAGAAA
TTGCTGGAGATAAAAACTTTAAAAAAATTGAAAAATATGGTGAATGTGATTTTTCTGTAGCTGTAGAAAGTGGAGAAAGA
TTTAGGGTAAATGCTTATAAACAAAAAGGAAGTTATGCTATTGCAATAAGAGCTATTACTTCTCAAATCCCTTCTTTCGA
TGATTTAGGATTGCCTGATATCCTTAAAAATTTTACAGAAAAGCATAAAGGGCTAGTATTAGTTACAGGGCCAACAGGAA
GTGGTAAAAGTACGACACTTGCTAGTCTCATAGACATAATAAATAAAAATCAACAAAGACATATTATAACTTTAGAAGAT
CCAATAGAATACGTTCATCAACATAAGAAAAGCTTAGTAAATCAAAGAGAAATAGGACAAGATACTGAAAGCTTTAACTC
TGCCCTAAAGGCAATACTTCGTCAAGATCCAGATGTTATTCTTGTAGGAGAAATGAGAGATTTAGAAACTATTTCTATAG
CACTAACAGCAGCAGAAACAGGTCACTTAGTGTTTTCTACTCTTCATACTATCGGGGCAGCTAAAACAATAGATAGAATA
GTAGATATGTTTCCATCAGAGCAACAGCAGCAAGTTAGAACTCAATTATCCACAGTATGTGAAGGGGTTATTTCTCAACA
GTTATTGCAGACTATTGATGGAAAAAATAGAGTTGTAGCTATGGAGGTTATGGTGTCAAATCCAGCTATAAGAAATTTAA
TAAGAGAAAATAAAACTTATCAAATTCAAAATATTATACAGACAGGAAGCAAATATGGAATGCAAACTATGGATCAAGAA
TTAGTTAATTTATATATGAAAGGAAAGATATCAAAGCATAGTGCGCTTAGTAGATGTACAGACTACGAATATACTTGTAG
ATTAGTTGGAGATAGATACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

51.94

94.901

0.493
  pilT Legionella pneumophila strain Lp02

51.94

94.901

0.493
  pilT Neisseria gonorrhoeae MS11

49.854

97.167

0.484
  pilT Neisseria meningitidis 8013

49.854

97.167

0.484
  pilT Acinetobacter baumannii D1279779

50.746

94.901

0.482
  pilT Acinetobacter nosocomialis M2

50.746

94.901

0.482
  pilT Acinetobacter baumannii strain A118

50.746

94.901

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.863

99.433

0.476
  pilT Pseudomonas stutzeri DSM 10701

49.851

94.901

0.473
  pilT Pseudomonas aeruginosa PAK

49.552

94.901

0.47
  pilT Acinetobacter baylyi ADP1

49.552

94.901

0.47
  pilT Vibrio cholerae strain A1552

47.929

95.751

0.459
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.929

95.751

0.459
  pilU Vibrio cholerae strain A1552

41.159

97.734

0.402
  pilU Acinetobacter baylyi ADP1

41.176

96.317

0.397
  pilU Pseudomonas stutzeri DSM 10701

39.583

95.184

0.377