Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SERAS_RS20435 Genome accession   NZ_AP019009
Coordinates   4275911..4276933 (-) Length   340 a.a.
NCBI ID   WP_060445093.1    Uniprot ID   -
Organism   Serratia marcescens strain AS-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4270911..4281933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SERAS_RS20405 (SERAS_39160) - 4271711..4272223 (+) 513 WP_117287304.1 SprT family zinc-dependent metalloprotease -
  SERAS_RS20410 (SERAS_39170) endA 4272325..4273020 (+) 696 WP_049300546.1 deoxyribonuclease I -
  SERAS_RS20415 (SERAS_39180) rsmE 4273090..4273821 (+) 732 WP_049300547.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  SERAS_RS20420 (SERAS_39190) gshB 4273832..4274782 (+) 951 WP_016930084.1 glutathione synthase -
  SERAS_RS20425 (SERAS_39210) - 4274929..4275492 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  SERAS_RS20430 (SERAS_39220) ruvX 4275492..4275914 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  SERAS_RS20435 (SERAS_39230) pilT 4275911..4276933 (-) 1023 WP_060445093.1 type IV pilus twitching motility protein PilT Machinery gene
  SERAS_RS20440 (SERAS_39240) - 4276954..4277661 (+) 708 WP_033639392.1 YggS family pyridoxal phosphate-dependent enzyme -
  SERAS_RS20445 (SERAS_39250) proC 4277681..4278502 (+) 822 WP_079450221.1 pyrroline-5-carboxylate reductase -
  SERAS_RS20450 (SERAS_39260) - 4278534..4279088 (+) 555 WP_016930090.1 YggT family protein -
  SERAS_RS20455 (SERAS_39270) yggU 4279085..4279378 (+) 294 WP_117287303.1 DUF167 family protein YggU -
  SERAS_RS20460 (SERAS_39280) - 4279519..4280112 (+) 594 WP_016930092.1 XTP/dITP diphosphatase -
  SERAS_RS20465 (SERAS_39290) hemW 4280105..4281247 (+) 1143 WP_048796774.1 radical SAM family heme chaperone HemW -
  SERAS_RS20470 (SERAS_39300) - 4281295..4281528 (-) 234 WP_370530994.1 DUF2442 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36488.78 Da        Isoelectric Point: 7.2889

>NTDB_id=72065 SERAS_RS20435 WP_060445093.1 4275911..4276933(-) (pilT) [Serratia marcescens strain AS-1]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANVFQQSAGISLALRRIAGQSPSLAELAAPAIVPALLRRDDGLILVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGECAGERAGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=72065 SERAS_RS20435 WP_060445093.1 4275911..4276933(-) (pilT) [Serratia marcescens strain AS-1]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGGGCGGGGAG
CGGTTGCGCGCCAACGTTTTTCAACAAAGCGCGGGGATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGACGACGGGCTTATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCATATTTTGACGCTGGAA
GATCCGATCGAGTTTCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCATACCCGCAGCGCGCCGCAGGCGGTAGAACGG
CTGGTGGACGTGTTTCCCGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCCGGCGGCGGACGCGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGTGCGCGGGGGAAAGGGCGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.848

97.059

0.532
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.848

97.059

0.532
  pilT Neisseria meningitidis 8013

50.294

100

0.503
  pilT Acinetobacter baylyi ADP1

51.36

97.353

0.5
  pilT Pseudomonas aeruginosa PAK

49.267

100

0.494
  pilT Acinetobacter nosocomialis M2

50.909

97.059

0.494
  pilT Acinetobacter baumannii strain A118

50.909

97.059

0.494
  pilT Acinetobacter baumannii D1279779

50.909

97.059

0.494
  pilT Neisseria gonorrhoeae MS11

49.704

99.412

0.494
  pilT Pseudomonas stutzeri DSM 10701

48.673

99.706

0.485
  pilT Legionella pneumophila strain Lp02

48.529

100

0.485
  pilT Legionella pneumophila strain ERS1305867

48.529

100

0.485
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.029

100

0.453
  pilU Pseudomonas stutzeri DSM 10701

38.643

99.706

0.385
  pilU Acinetobacter baylyi ADP1

40.248

95

0.382
  pilU Vibrio cholerae strain A1552

39.877

95.882

0.382

Multiple sequence alignment