Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PFJ30894_RS07345 Genome accession   NZ_AP019004
Coordinates   1497547..1498587 (+) Length   346 a.a.
NCBI ID   WP_113077357.1    Uniprot ID   -
Organism   Phascolarctobacterium faecium strain JCM 30894     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1492547..1503587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PFJ30894_RS07325 (PFJ30894_01490) thiE 1492958..1493596 (+) 639 WP_113077358.1 thiamine phosphate synthase -
  PFJ30894_RS07330 (PFJ30894_01491) - 1493646..1495091 (-) 1446 WP_113077076.1 secretin N-terminal domain-containing protein -
  PFJ30894_RS07335 (PFJ30894_01492) - 1495091..1495609 (-) 519 WP_113077075.1 hypothetical protein -
  PFJ30894_RS07340 (PFJ30894_01493) gspE 1495849..1497519 (+) 1671 WP_021718325.1 type II secretion system ATPase GspE -
  PFJ30894_RS07345 (PFJ30894_01494) pilT 1497547..1498587 (+) 1041 WP_113077357.1 type IV pilus twitching motility protein PilT Machinery gene
  PFJ30894_RS07350 (PFJ30894_01495) - 1498609..1499823 (+) 1215 WP_125669430.1 type II secretion system F family protein -
  PFJ30894_RS07355 (PFJ30894_01496) - 1499960..1500445 (+) 486 WP_125669432.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PFJ30894_RS12040 (PFJ30894_01497) - 1500509..1500970 (+) 462 WP_113077068.1 type II secretion system protein -
  PFJ30894_RS07365 (PFJ30894_01498) - 1501321..1501881 (+) 561 WP_125669433.1 hypothetical protein -
  PFJ30894_RS07370 (PFJ30894_01499) - 1501874..1502389 (+) 516 WP_113077070.1 hypothetical protein -
  PFJ30894_RS07375 (PFJ30894_01500) - 1502480..1502968 (-) 489 WP_113077071.1 type IV pilin protein -
  PFJ30894_RS12045 (PFJ30894_01501) - 1503036..1503419 (-) 384 WP_211305758.1 type IV pilin protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38814.35 Da        Isoelectric Point: 6.5016

>NTDB_id=72024 PFJ30894_RS07345 WP_113077357.1 1497547..1498587(+) (pilT) [Phascolarctobacterium faecium strain JCM 30894]
MRDAFDVNASDIHLTVGLPPMFRVNGVLRQAGEEPLRREDTYNMAMELMDDRRRTQFLDNGEADFSYEIENVCRFRVNVF
KQSNCVAAALRLISNNIPSFEDLRLPDVISDLAMLPRGLVLVTGPTGSGKSTTLAAVINKINKHRKDHVITLEDPIEYKH
NHINSIINQREVGSDTKSFANGLRAALREDPDVILVGEMRDTETIATAITAAETGHLVLATLHTQDAASTVNRIIDVFPE
HQQQQIRIQLAGNLQGIVAQQLLMNREQTGRLAAFEILVATPALRNLIREGKTHQIPSYIQTGSKFGMICMDAYLAQLVR
KNKIDRSIAEERCYDPLSLERYMHGL

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=72024 PFJ30894_RS07345 WP_113077357.1 1497547..1498587(+) (pilT) [Phascolarctobacterium faecium strain JCM 30894]
ATGCGTGACGCTTTTGATGTCAACGCTTCGGATATCCATTTAACGGTAGGCTTGCCGCCAATGTTCCGTGTCAATGGCGT
GCTGCGGCAGGCGGGTGAAGAACCTCTGCGCCGTGAGGATACCTATAATATGGCCATGGAACTGATGGACGATCGGCGCC
GCACGCAGTTTCTTGATAACGGCGAAGCTGATTTTTCTTATGAAATTGAAAATGTATGCCGTTTCCGTGTTAATGTTTTT
AAGCAGAGTAATTGTGTGGCAGCAGCGCTGCGTTTGATTTCCAATAATATACCCAGCTTTGAGGACCTGCGCCTGCCTGA
CGTTATCAGCGACCTTGCCATGCTGCCGCGTGGTCTGGTATTGGTGACAGGGCCTACAGGCAGCGGTAAATCAACGACAC
TGGCGGCTGTTATCAATAAAATCAACAAACACCGCAAAGATCATGTCATCACTCTGGAAGATCCGATCGAATATAAGCAT
AACCATATCAACAGCATTATCAACCAGCGCGAAGTCGGCAGTGATACCAAAAGCTTTGCCAATGGCCTTAGGGCAGCATT
ACGTGAAGATCCTGACGTTATTCTGGTAGGCGAGATGCGTGATACTGAAACTATCGCCACCGCTATCACTGCTGCCGAAA
CCGGACATCTTGTTTTGGCAACTCTGCATACTCAGGATGCGGCATCGACAGTGAATCGTATCATCGACGTCTTTCCCGAA
CACCAGCAGCAGCAGATTCGCATTCAGCTGGCTGGCAACCTGCAGGGCATCGTTGCCCAGCAACTGCTGATGAATCGCGA
ACAAACCGGACGTCTGGCTGCTTTTGAAATATTAGTTGCCACACCGGCGCTGCGGAACTTGATCCGCGAAGGCAAAACAC
ATCAGATACCGTCATATATCCAGACTGGTTCCAAATTCGGGATGATTTGTATGGATGCCTATCTGGCGCAGCTGGTACGT
AAAAATAAGATCGACCGCAGTATAGCGGAAGAACGCTGTTATGACCCATTATCATTAGAACGCTATATGCATGGACTATA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(70-263)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.666

98.555

0.529
  pilT Legionella pneumophila strain Lp02

53.03

95.376

0.506
  pilT Legionella pneumophila strain ERS1305867

53.03

95.376

0.506
  pilT Acinetobacter baylyi ADP1

50

97.688

0.488
  pilT Neisseria meningitidis 8013

51.057

95.665

0.488
  pilT Neisseria gonorrhoeae MS11

50.755

95.665

0.486
  pilT Acinetobacter nosocomialis M2

48.817

97.688

0.477
  pilT Acinetobacter baumannii D1279779

48.817

97.688

0.477
  pilT Acinetobacter baumannii strain A118

48.817

97.688

0.477
  pilT Vibrio cholerae strain A1552

51.572

91.908

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.572

91.908

0.474
  pilT Pseudomonas aeruginosa PAK

48.328

95.087

0.46
  pilT Pseudomonas stutzeri DSM 10701

48.024

95.087

0.457
  pilU Vibrio cholerae strain A1552

44.164

91.618

0.405
  pilU Pseudomonas stutzeri DSM 10701

42.901

93.642

0.402
  pilU Acinetobacter baylyi ADP1

38.554

95.954

0.37

Multiple sequence alignment