Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NVV94_RS25155 Genome accession   NZ_CP102951
Coordinates   5374663..5375697 (-) Length   344 a.a.
NCBI ID   WP_258444982.1    Uniprot ID   -
Organism   Pseudomonas sp. LS1212     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5369663..5380697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NVV94_RS25125 (NVV94_25125) ruvX 5369941..5370381 (+) 441 WP_258444977.1 Holliday junction resolvase RuvX -
  NVV94_RS25130 (NVV94_25130) pyrR 5370436..5370942 (+) 507 WP_258444978.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  NVV94_RS25135 (NVV94_25135) - 5370955..5371959 (+) 1005 WP_166361351.1 aspartate carbamoyltransferase catalytic subunit -
  NVV94_RS25140 (NVV94_25140) - 5371956..5373227 (+) 1272 WP_258444979.1 dihydroorotase -
  NVV94_RS25145 (NVV94_25145) - 5373327..5373746 (-) 420 WP_258444980.1 TM2 domain-containing protein -
  NVV94_RS25150 (NVV94_25150) - 5373995..5374618 (+) 624 WP_258444981.1 C40 family peptidase -
  NVV94_RS25155 (NVV94_25155) pilT 5374663..5375697 (-) 1035 WP_258444982.1 type IV pilus twitching motility protein PilT Machinery gene
  NVV94_RS25160 (NVV94_25160) - 5375763..5376449 (+) 687 WP_258444983.1 YggS family pyridoxal phosphate-dependent enzyme -
  NVV94_RS25165 (NVV94_25165) proC 5376486..5377304 (+) 819 WP_258444984.1 pyrroline-5-carboxylate reductase -
  NVV94_RS25170 (NVV94_25170) - 5377315..5377905 (+) 591 WP_258444985.1 YggT family protein -
  NVV94_RS25175 (NVV94_25175) - 5377905..5378192 (+) 288 WP_258444986.1 DUF167 domain-containing protein -
  NVV94_RS25180 (NVV94_25180) - 5378341..5379480 (+) 1140 WP_258444987.1 homoserine O-acetyltransferase -
  NVV94_RS25185 (NVV94_25185) metW 5379488..5380108 (+) 621 WP_258444988.1 methionine biosynthesis protein MetW -
  NVV94_RS25190 (NVV94_25190) - 5380134..5380571 (+) 438 WP_258444989.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37846.62 Da        Isoelectric Point: 6.5764

>NTDB_id=720187 NVV94_RS25155 WP_258444982.1 5374663..5375697(-) (pilT) [Pseudomonas sp. LS1212]
MDVTELLAFGARQGASDLHLSAGLPPMIRVDGDMRRINLPALDHEQVHELIDAIMNEKQRKNFEACLEIDFSFEIPDVAR
FRVNAFHQSRGAGAVFRTIPAKVLSMEDLGMGDVFRRIADMPRGLVLVTGPTGSGKSTTLAAMVDYLNNTRHRHILTIED
PIEFVHESRKCLINQREVHCDTLGFGNALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSASKTIDRV
VDVFPAEEKSMVRSMLSESLQAVVSQVLVRKMGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSSIQTGGALGMRTLDMSL
KDLLAKGVIARDSAMEKARSPEGF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=720187 NVV94_RS25155 WP_258444982.1 5374663..5375697(-) (pilT) [Pseudomonas sp. LS1212]
ATGGACGTTACCGAGCTGCTGGCCTTCGGCGCCAGGCAAGGCGCCTCCGACCTGCACCTTTCGGCAGGGCTGCCGCCGAT
GATCCGGGTAGACGGTGATATGCGTCGCATCAATCTTCCGGCCCTGGACCACGAACAGGTCCACGAGCTGATTGACGCCA
TCATGAACGAAAAACAGCGCAAGAACTTCGAAGCCTGTCTGGAGATCGACTTTTCCTTCGAAATCCCGGATGTGGCGCGC
TTTCGGGTCAATGCCTTCCATCAGAGTCGGGGCGCCGGTGCCGTGTTTCGAACCATTCCGGCCAAGGTCCTGAGCATGGA
GGACCTTGGCATGGGAGATGTGTTTCGCAGGATTGCCGACATGCCCAGGGGGTTGGTGTTGGTCACCGGTCCTACCGGCT
CCGGTAAGTCGACGACGTTGGCAGCCATGGTCGATTACCTCAATAACACCAGGCATCGGCATATTCTGACCATCGAAGAC
CCGATCGAATTTGTTCACGAATCGCGCAAATGCTTGATCAATCAGCGCGAAGTGCACTGCGATACCCTGGGCTTCGGCAA
TGCACTGCGTTCGGCGCTGCGTGAGGACCCGGATGTCATCCTGGTTGGCGAAATGCGTGACCTGGAAACCATTCGCCTGG
CGCTGACAGCGGCAGAAACCGGGCATCTGGTGTTCGGTACGCTACACACCACCTCGGCGTCGAAAACCATCGACCGGGTT
GTGGACGTTTTTCCCGCCGAGGAAAAATCCATGGTTCGTTCGATGTTGTCGGAATCCTTGCAGGCGGTGGTTTCCCAGGT
GCTCGTCAGGAAGATGGGGGGCGGCCGGGTCGCCGCTCACGAAATCATGCTGGGCACGTCGGCGATTCGAAACCTGATAC
GCGAAGACAAGGTCGCGCAGATGTATTCCTCGATCCAGACCGGCGGAGCATTGGGCATGCGGACGCTGGATATGAGCCTC
AAGGATCTGCTCGCCAAGGGTGTGATTGCGCGTGACAGCGCAATGGAAAAGGCCAGGTCGCCAGAGGGTTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

84.302

100

0.843
  pilT Pseudomonas aeruginosa PAK

82.267

100

0.823
  pilT Acinetobacter baylyi ADP1

76.453

100

0.765
  pilT Acinetobacter baumannii strain A118

76.163

100

0.762
  pilT Acinetobacter baumannii D1279779

76.163

100

0.762
  pilT Acinetobacter nosocomialis M2

75.872

100

0.759
  pilT Legionella pneumophila strain Lp02

70.058

100

0.701
  pilT Legionella pneumophila strain ERS1305867

70.058

100

0.701
  pilT Neisseria meningitidis 8013

65.889

99.709

0.657
  pilT Neisseria gonorrhoeae MS11

65.598

99.709

0.654
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.085

95.64

0.651
  pilT Vibrio cholerae strain A1552

68.085

95.64

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Pseudomonas stutzeri DSM 10701

40.762

99.128

0.404
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395
  pilU Vibrio cholerae strain A1552

39.589

99.128

0.392