Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NWV12_RS18375 Genome accession   NZ_CP102932
Coordinates   3878739..3879719 (-) Length   326 a.a.
NCBI ID   WP_101744680.1    Uniprot ID   -
Organism   Enterobacter sp. Crenshaw     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3873739..3884719
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NWV12_RS18345 (NWV12_18345) - 3874615..3875112 (+) 498 WP_048979369.1 SprT family zinc-dependent metalloprotease -
  NWV12_RS18350 (NWV12_18350) endA 3875207..3875914 (+) 708 WP_048979370.1 deoxyribonuclease I -
  NWV12_RS18355 (NWV12_18355) rsmE 3875966..3876697 (+) 732 WP_023309126.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NWV12_RS18360 (NWV12_18360) gshB 3876717..3877664 (+) 948 WP_029739222.1 glutathione synthase -
  NWV12_RS18365 (NWV12_18365) - 3877752..3878312 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  NWV12_RS18370 (NWV12_18370) ruvX 3878312..3878728 (+) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  NWV12_RS18375 (NWV12_18375) pilT 3878739..3879719 (-) 981 WP_101744680.1 type IV pilus twitching motility protein PilT Machinery gene
  NWV12_RS18380 (NWV12_18380) - 3879737..3880438 (+) 702 WP_048979373.1 YggS family pyridoxal phosphate-dependent enzyme -
  NWV12_RS18385 (NWV12_18385) - 3880460..3881026 (+) 567 WP_023309130.1 YggT family protein -
  NWV12_RS18390 (NWV12_18390) yggU 3881023..3881319 (+) 297 WP_023309131.1 DUF167 family protein YggU -
  NWV12_RS18395 (NWV12_18395) - 3881323..3881916 (+) 594 WP_048979374.1 XTP/dITP diphosphatase -
  NWV12_RS18400 (NWV12_18400) hemW 3881909..3883045 (+) 1137 WP_048979375.1 radical SAM family heme chaperone HemW -
  NWV12_RS18405 (NWV12_18405) - 3883115..3883477 (+) 363 WP_075204767.1 endonuclease domain-containing protein -
  NWV12_RS18410 (NWV12_18410) - 3883539..3884255 (-) 717 WP_024906551.1 DUF2884 domain-containing protein -
  NWV12_RS18415 (NWV12_18415) - 3884312..3884638 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35436.46 Da        Isoelectric Point: 6.6964

>NTDB_id=719847 NWV12_RS18375 WP_101744680.1 3878739..3879719(-) (pilT) [Enterobacter sp. Crenshaw]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGTWWAKGQVDFAATVTGGQRLRG
SAFKQMSGISVTLRLLPRSCPQLASLGAPRVIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLIPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=719847 NWV12_RS18375 WP_101744680.1 3878739..3879719(-) (pilT) [Enterobacter sp. Crenshaw]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATTAGGCCGGCTTGAACCCGCTCCCTTTCCGCCTCCCGATGTCGGAGCGTTATTAAAAGCGTGGCTCAACG
ATGAGCAGCAGGGAACATGGTGGGCAAAGGGCCAGGTGGATTTTGCCGCGACGGTAACGGGAGGCCAGCGTCTGCGCGGC
AGTGCCTTTAAGCAGATGAGCGGTATCTCTGTGACGCTGCGGCTGTTGCCGCGTAGCTGCCCGCAGCTCGCTTCGCTGGG
CGCGCCGAGGGTAATCCCGGAACTGTTATCCAATGACGCCGGGCTGATTCTGGTCACGGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTAAACCACCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAGCGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCTGAAGCGCTGCG
CAGCGCCTTACGCCAGGATCCGGACGTTATTCTGCTGGGGGAACTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGACATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAAAGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAAGCTGATTCC
CGATCTACAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACTGCGGCGGCGGCGAATTTGATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGGATCATTCAAACCGGTCAGCAGGCAGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(78-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Pseudomonas aeruginosa PAK

48.012

100

0.482
  pilT Acinetobacter baumannii strain A118

47.706

100

0.479
  pilT Acinetobacter baumannii D1279779

47.706

100

0.479
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

48.125

98.16

0.472
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.168

98.773

0.426
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365