Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EZJ49_RS05975 Genome accession   NZ_CP102930
Coordinates   1189034..1190134 (-) Length   366 a.a.
NCBI ID   WP_262555209.1    Uniprot ID   -
Organism   Bdellovibrio bacteriovorus strain kdesi     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1184034..1195134
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZJ49_RS05950 (EZJ49_05950) - 1184394..1185623 (-) 1230 WP_262555204.1 S8 family peptidase -
  EZJ49_RS05955 (EZJ49_05955) - 1185768..1186583 (-) 816 WP_262555205.1 purine-nucleoside phosphorylase -
  EZJ49_RS05960 (EZJ49_05960) - 1186613..1187932 (-) 1320 WP_262555206.1 thymidine phosphorylase -
  EZJ49_RS05965 (EZJ49_05965) deoC 1187932..1188594 (-) 663 WP_262555207.1 deoxyribose-phosphate aldolase -
  EZJ49_RS05970 (EZJ49_05970) - 1188654..1189028 (+) 375 WP_262555208.1 hypothetical protein -
  EZJ49_RS05975 (EZJ49_05975) pilT 1189034..1190134 (-) 1101 WP_262555209.1 type IV pilus twitching motility protein PilT Machinery gene
  EZJ49_RS05980 (EZJ49_05980) - 1190272..1191789 (+) 1518 WP_262555210.1 ribonuclease E/G -
  EZJ49_RS05985 (EZJ49_05985) - 1191870..1193477 (+) 1608 WP_262555212.1 hypothetical protein -
  EZJ49_RS05990 (EZJ49_05990) rplU 1193685..1194248 (+) 564 WP_262555214.1 50S ribosomal protein L21 -
  EZJ49_RS05995 (EZJ49_05995) rpmA 1194278..1194538 (+) 261 WP_063205653.1 50S ribosomal protein L27 -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 39690.73 Da        Isoelectric Point: 7.9844

>NTDB_id=719811 EZJ49_RS05975 WP_262555209.1 1189034..1190134(-) (pilT) [Bdellovibrio bacteriovorus strain kdesi]
MATIDELFKLMVEQGASDLHITSGAAPYLRLHGNMVPLNYRELTNQDVQGLLFEILSEKQKKAFVEKWELDFAYTLPGTG
RFRCNIFMQRKGLGAVMRIIPEKIKTAQELGVPPSVMDIIDCDRGLILVTGPTGSGKSTTLAAMIHQINMTREAHIITVE
DPIEFVHPNLKSLVNQREVGSHTKSFANALKAALREDPDILLVGELRDLETISLALTAAETGHIVFGTLHTNSAAKTIDR
IIDVFPAGQQQQIRTMLAESLRGVVAQTLFSRADGQGRVAAYEIMRNTKAISNLIREGKVHQIPSAMQTGSSQGMVLFEK
YIEDLVRKGKVSAADAKTFLGQAGGDTSVQTAVGGGAAGGPRTKVG

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=719811 EZJ49_RS05975 WP_262555209.1 1189034..1190134(-) (pilT) [Bdellovibrio bacteriovorus strain kdesi]
ATGGCAACAATTGATGAACTGTTTAAACTCATGGTCGAACAAGGAGCTTCCGACTTGCACATCACAAGCGGAGCCGCCCC
TTATTTGCGCCTGCATGGAAACATGGTTCCATTGAACTATCGCGAACTCACGAACCAAGACGTTCAGGGTTTGCTTTTTG
AGATCCTTTCAGAAAAGCAAAAGAAGGCCTTCGTTGAAAAATGGGAGCTGGATTTCGCCTACACACTTCCCGGCACAGGT
CGTTTCCGTTGCAATATCTTTATGCAGCGTAAAGGTCTTGGCGCTGTTATGCGTATCATTCCTGAAAAAATCAAAACCGC
ACAAGAACTGGGTGTTCCACCTTCTGTGATGGACATCATCGATTGCGATCGTGGTCTGATTCTGGTGACGGGTCCTACCG
GCTCTGGTAAGTCCACCACTTTGGCAGCGATGATTCACCAGATTAACATGACCCGTGAAGCACACATCATCACGGTGGAA
GATCCTATCGAGTTCGTTCACCCGAACTTGAAATCTTTGGTGAATCAGCGTGAGGTCGGAAGTCACACCAAAAGTTTCGC
CAATGCCCTTAAAGCGGCCTTGCGTGAAGACCCGGATATCCTGCTGGTGGGTGAGTTGCGTGACTTGGAAACAATCTCTT
TAGCCCTGACGGCAGCGGAAACAGGACACATTGTGTTCGGAACCCTGCATACTAACAGTGCTGCCAAAACCATTGACCGT
ATCATCGACGTTTTCCCTGCGGGACAACAACAGCAGATCCGCACGATGCTGGCCGAATCCCTGCGTGGAGTTGTCGCTCA
GACGCTGTTCTCCCGCGCGGACGGACAAGGTCGCGTGGCTGCTTACGAGATCATGAGAAATACCAAGGCGATTTCCAATT
TGATTCGTGAAGGTAAGGTCCACCAAATCCCATCTGCGATGCAGACTGGCTCCAGCCAGGGGATGGTGTTGTTCGAAAAA
TACATTGAAGATCTGGTCCGCAAAGGAAAGGTTTCCGCAGCCGATGCAAAAACCTTCCTGGGTCAGGCTGGTGGCGATAC
CAGTGTGCAGACAGCCGTTGGTGGCGGAGCTGCCGGCGGTCCGCGCACTAAAGTGGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-258)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

55.389

91.257

0.505
  pilT Pseudomonas stutzeri DSM 10701

55.09

91.257

0.503
  pilT Acinetobacter baumannii strain A118

54.925

91.53

0.503
  pilT Acinetobacter nosocomialis M2

54.925

91.53

0.503
  pilT Acinetobacter baumannii D1279779

54.925

91.53

0.503
  pilT Vibrio cholerae strain A1552

54.627

91.53

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.627

91.53

0.5
  pilT Acinetobacter baylyi ADP1

54.491

91.257

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.77

93.716

0.495
  pilT Legionella pneumophila strain Lp02

54.103

89.891

0.486
  pilT Legionella pneumophila strain ERS1305867

54.103

89.891

0.486
  pilT Neisseria gonorrhoeae MS11

51.497

91.257

0.47
  pilT Neisseria meningitidis 8013

51.497

91.257

0.47
  pilU Pseudomonas stutzeri DSM 10701

41.543

92.077

0.383
  pilU Acinetobacter baylyi ADP1

41.018

91.257

0.374
  pilU Vibrio cholerae strain A1552

41.104

89.071

0.366