Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NVB75_RS18045 Genome accession   NZ_CP102929
Coordinates   3932363..3933397 (-) Length   344 a.a.
NCBI ID   WP_258342641.1    Uniprot ID   -
Organism   Pseudomonas sp. CBS     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3927363..3938397
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NVB75_RS18015 (NVB75_18015) ruvX 3927384..3927821 (+) 438 WP_003213744.1 Holliday junction resolvase RuvX -
  NVB75_RS18020 (NVB75_18020) pyrR 3927996..3928502 (+) 507 WP_167664095.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  NVB75_RS18025 (NVB75_18025) - 3928527..3929531 (+) 1005 WP_167664094.1 aspartate carbamoyltransferase catalytic subunit -
  NVB75_RS18030 (NVB75_18030) - 3929528..3930799 (+) 1272 WP_063026517.1 dihydroorotase -
  NVB75_RS18035 (NVB75_18035) - 3930934..3931362 (-) 429 WP_076012988.1 TM2 domain-containing protein -
  NVB75_RS18040 (NVB75_18040) - 3931612..3932247 (+) 636 WP_076012989.1 C40 family peptidase -
  NVB75_RS18045 (NVB75_18045) pilT 3932363..3933397 (-) 1035 WP_258342641.1 type IV pilus twitching motility protein PilT Machinery gene
  NVB75_RS18050 (NVB75_18050) - 3933455..3934141 (+) 687 WP_076012991.1 YggS family pyridoxal phosphate-dependent enzyme -
  NVB75_RS18055 (NVB75_18055) proC 3934175..3934993 (+) 819 WP_258342643.1 pyrroline-5-carboxylate reductase -
  NVB75_RS18060 (NVB75_18060) - 3935018..3935605 (+) 588 WP_017476523.1 YggT family protein -
  NVB75_RS18065 (NVB75_18065) - 3935835..3936973 (+) 1139 Protein_3574 homoserine O-acetyltransferase -
  NVB75_RS18070 (NVB75_18070) metW 3936981..3937601 (+) 621 WP_076012994.1 methionine biosynthesis protein MetW -
  NVB75_RS18075 (NVB75_18075) - 3937623..3938057 (+) 435 WP_258342645.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37613.36 Da        Isoelectric Point: 7.1311

>NTDB_id=719797 NVB75_RS18045 WP_258342641.1 3932363..3933397(-) (pilT) [Pseudomonas sp. CBS]
MDITELLAAGVRRRASDLHLSAGLAPMLRVDGEVWPLDWPVLLPTQVAELLSPLLNNHQQKDFETSLETDFAFELPEVAR
FRVNVFQQHRGMGAVFRTIPSAVQSLESLGLGEVFRRIADLPRGLALVTGPTGSGKSTTLAAMIDYLNTHRRQHILTLED
PIEFIHQPKSALINQRQVHRDTHSFSAALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTVDRV
VDVFPAGEKAMVRSMLSESLQAVVSQVLVKKVGGGRVAAHEIMLGTPAIRNLIREDKVAQMYSAIQTGGALGMTTLDMSL
KALVSQGLISREHAREKARVPGDI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=719797 NVB75_RS18045 WP_258342641.1 3932363..3933397(-) (pilT) [Pseudomonas sp. CBS]
ATGGATATCACTGAACTGCTGGCTGCCGGCGTACGCCGGCGGGCCTCGGACCTGCATCTGTCGGCCGGGTTGGCGCCGAT
GCTGCGAGTTGATGGCGAGGTCTGGCCCCTCGATTGGCCGGTGCTGCTCCCTACGCAAGTGGCGGAGTTACTCAGCCCTT
TGCTGAACAATCATCAGCAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGAGTTGCCCGAGGTCGCACGG
TTTCGGGTAAATGTGTTCCAGCAGCACCGGGGCATGGGCGCGGTGTTTCGCACCATCCCCTCGGCGGTGCAGAGCCTGGA
AAGCCTGGGGTTGGGCGAGGTGTTCCGGCGCATCGCGGACCTGCCCCGAGGGCTGGCACTGGTCACCGGGCCGACCGGTT
CCGGCAAGTCCACGACCCTGGCGGCGATGATCGATTACCTCAACACCCATCGCCGCCAGCACATCCTCACCCTTGAAGAC
CCTATCGAATTTATCCATCAACCGAAAAGCGCCCTGATCAACCAGCGGCAGGTGCATCGCGACACCCACAGTTTCTCGGC
CGCTCTGCGTTCGGCATTACGGGAGGACCCGGATGTGATCCTGGTGGGCGAATTGCGGGACCTGGAAACCATTCGGCTCG
CACTGACGGCGGCGGAAACCGGGCACCTGGTGTTCGGCACATTGCACACCACGTCGGCGGCTAAAACCGTGGATCGGGTG
GTGGATGTGTTTCCGGCAGGGGAAAAGGCCATGGTCCGCTCGATGCTCTCGGAGTCATTGCAGGCGGTGGTCTCTCAGGT
GCTGGTGAAGAAGGTCGGCGGCGGGCGGGTGGCGGCTCACGAAATCATGCTCGGCACGCCGGCAATTCGTAACTTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACGGGTGGGGCGTTGGGGATGACGACGCTGGACATGAGTTTG
AAGGCGTTGGTCAGCCAGGGGCTGATCAGCCGGGAGCATGCGCGGGAGAAGGCGAGGGTGCCCGGGGATATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.367

99.128

0.747
  pilT Pseudomonas stutzeri DSM 10701

73.9

99.128

0.733
  pilT Acinetobacter nosocomialis M2

72.141

99.128

0.715
  pilT Acinetobacter baumannii D1279779

71.848

99.128

0.712
  pilT Acinetobacter baumannii strain A118

71.848

99.128

0.712
  pilT Acinetobacter baylyi ADP1

71.554

99.128

0.709
  pilT Legionella pneumophila strain Lp02

65.385

98.256

0.642
  pilT Legionella pneumophila strain ERS1305867

65.385

98.256

0.642
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.688

97.965

0.634
  pilT Vibrio cholerae strain A1552

64.688

97.965

0.634
  pilT Neisseria meningitidis 8013

63.824

98.837

0.631
  pilT Neisseria gonorrhoeae MS11

63.529

98.837

0.628
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.708

99.419

0.494
  pilU Pseudomonas stutzeri DSM 10701

39.42

100

0.395
  pilU Vibrio cholerae strain A1552

38.551

100

0.387
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387