Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NWT83_RS03625 Genome accession   NZ_CP102898
Coordinates   731549..732529 (+) Length   326 a.a.
NCBI ID   WP_044524702.1    Uniprot ID   A0AAN2CC74
Organism   Klebsiella quasipneumoniae strain KW1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 726549..737529
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NWT83_RS03595 (NWT83_03595) - 726931..727917 (-) 987 WP_065882670.1 TRAP transporter substrate-binding protein -
  NWT83_RS03600 (NWT83_03600) hemW 728222..729358 (-) 1137 WP_065882671.1 radical SAM family heme chaperone HemW -
  NWT83_RS03605 (NWT83_03605) - 729351..729944 (-) 594 WP_023290995.1 XTP/dITP diphosphatase -
  NWT83_RS03610 (NWT83_03610) yggU 729957..730247 (-) 291 WP_004205183.1 DUF167 family protein YggU -
  NWT83_RS03615 (NWT83_03615) - 730244..730810 (-) 567 WP_002916613.1 YggT family protein -
  NWT83_RS03620 (NWT83_03620) - 730831..731532 (-) 702 WP_004205181.1 YggS family pyridoxal phosphate-dependent enzyme -
  NWT83_RS03625 (NWT83_03625) pilT 731549..732529 (+) 981 WP_044524702.1 type IV pilus twitching motility protein PilT Machinery gene
  NWT83_RS03630 (NWT83_03630) - 732878..733531 (+) 654 WP_032454048.1 LuxR C-terminal-related transcriptional regulator -
  NWT83_RS03635 (NWT83_03635) ruvX 733583..733999 (-) 417 WP_004205178.1 Holliday junction resolvase RuvX -
  NWT83_RS03640 (NWT83_03640) - 733999..734562 (-) 564 WP_002916603.1 YqgE/AlgH family protein -
  NWT83_RS03645 (NWT83_03645) gshB 734737..735687 (-) 951 WP_004205176.1 glutathione synthase -
  NWT83_RS03650 (NWT83_03650) rsmE 735708..736439 (-) 732 WP_065882672.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NWT83_RS03655 (NWT83_03655) endA 736531..737238 (-) 708 WP_032454045.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35383.79 Da        Isoelectric Point: 6.8575

>NTDB_id=719604 NWT83_RS03625 WP_044524702.1 731549..732529(+) (pilT) [Klebsiella quasipneumoniae strain KW1]
MKLEEIVALSVKHNVSDLHLCNSAAPRWRRQGKLESAPFPAPDIADLLNDWLDAAQFQHWQEHGQIDFALTLACGSRLRA
SAFAHTRGISLVLRLLPVQCPRLETLGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVGHLNQHLDGHILTLEDPVE
FIHHSERCLIQQREIGRHCPSFSAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVERLIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPAVANLIREGKVHQLPGVMQTGMQAGMLTFTQSFQQR
VAAGQL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=719604 NWT83_RS03625 WP_044524702.1 731549..732529(+) (pilT) [Klebsiella quasipneumoniae strain KW1]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATTCCGCCGCACCGCG
CTGGCGGCGGCAGGGCAAGCTGGAGTCCGCTCCCTTTCCCGCGCCGGATATCGCGGATTTACTCAACGACTGGCTCGATG
CCGCACAGTTCCAGCACTGGCAGGAGCACGGCCAGATTGACTTTGCGCTGACTCTGGCCTGCGGTTCGCGGCTACGCGCC
AGCGCTTTCGCCCATACGCGCGGAATATCGCTGGTGCTGCGCCTGCTGCCCGTCCAGTGCCCGCGTCTGGAGACGCTGGG
CGCGCCGCCTGCGCTAAGCGAACTACTGGCGGAAGAGAGTGGCTTGCTGCTGGTCACCGGGGCGACGGGCAGCGGCAAGT
CGACTACCCTGGCGGCGATGGTCGGGCATCTCAACCAGCATCTTGATGGCCATATTCTGACGCTGGAGGATCCGGTGGAG
TTTATTCATCACAGCGAGCGCTGCCTGATCCAGCAGCGGGAGATTGGCCGTCACTGTCCTTCGTTCTCCGCGGCGCTTCG
GGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGCTTGGGGAATTACGCGACAGCGAAACCATCCGCCTGGCGTTGACGG
CTGCGGAAACCGGGCATCTTGTGATGGCGACATTACACACCCGCGGCGCGGCGCCGGCGGTGGAGAGATTGATCGATGTC
TTTCCGGCAGAGGAGAAAGATCAGGTTCGTAGTCAGCTGGCCGGAAGCCTGTGCGCGGTGCTGGCGCAAAAATTATTACC
CGCACGTCAGGGCGGCAGAGTTGCGCTATACGAGCTTCTGGTCAATACGCCGGCGGTGGCGAATTTGATCCGCGAAGGAA
AAGTGCACCAGTTGCCCGGTGTTATGCAGACCGGGATGCAGGCCGGAATGCTGACATTTACCCAGAGTTTTCAGCAACGT
GTGGCCGCAGGCCAGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.682

100

0.518
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.682

100

0.518
  pilT Acinetobacter baylyi ADP1

48.624

100

0.488
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Acinetobacter nosocomialis M2

47.706

100

0.479
  pilT Legionella pneumophila strain ERS1305867

47.84

99.387

0.475
  pilT Legionella pneumophila strain Lp02

47.84

99.387

0.475
  pilT Pseudomonas stutzeri DSM 10701

47.401

100

0.475
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.164

97.239

0.429
  pilU Vibrio cholerae strain A1552

40.426

100

0.408
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

39.252

98.466

0.386