Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NWT75_RS03645 Genome accession   NZ_CP102893
Coordinates   737625..738605 (+) Length   326 a.a.
NCBI ID   WP_258336473.1    Uniprot ID   -
Organism   Klebsiella quasipneumoniae strain KA1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 732625..743605
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NWT75_RS03615 (NWT75_03615) - 733007..733993 (-) 987 WP_065882670.1 TRAP transporter substrate-binding protein -
  NWT75_RS03620 (NWT75_03620) hemW 734298..735434 (-) 1137 WP_065882671.1 radical SAM family heme chaperone HemW -
  NWT75_RS03625 (NWT75_03625) - 735427..736020 (-) 594 WP_023290995.1 XTP/dITP diphosphatase -
  NWT75_RS03630 (NWT75_03630) yggU 736033..736323 (-) 291 WP_004205183.1 DUF167 family protein YggU -
  NWT75_RS03635 (NWT75_03635) - 736320..736886 (-) 567 WP_002916613.1 YggT family protein -
  NWT75_RS03640 (NWT75_03640) - 736907..737608 (-) 702 WP_004205181.1 YggS family pyridoxal phosphate-dependent enzyme -
  NWT75_RS03645 (NWT75_03645) pilT 737625..738605 (+) 981 WP_258336473.1 type IV pilus twitching motility protein PilT Machinery gene
  NWT75_RS03650 (NWT75_03650) - 738954..739607 (+) 654 WP_032454048.1 LuxR C-terminal-related transcriptional regulator -
  NWT75_RS03655 (NWT75_03655) ruvX 739659..740075 (-) 417 WP_004205178.1 Holliday junction resolvase RuvX -
  NWT75_RS03660 (NWT75_03660) - 740075..740638 (-) 564 WP_002916603.1 YqgE/AlgH family protein -
  NWT75_RS03665 (NWT75_03665) gshB 740813..741763 (-) 951 WP_004205176.1 glutathione synthase -
  NWT75_RS03670 (NWT75_03670) rsmE 741784..742515 (-) 732 WP_065882672.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NWT75_RS03675 (NWT75_03675) endA 742607..743314 (-) 708 WP_032454045.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35340.76 Da        Isoelectric Point: 6.7310

>NTDB_id=719580 NWT75_RS03645 WP_258336473.1 737625..738605(+) (pilT) [Klebsiella quasipneumoniae strain KA1]
MKLEEIVALSVKHNVSDLHLCNSAAPRWRRQGKLESAPFPAPDIADLLNDWLDAAQFQHWQEHGQIDFALTLACGSRLRA
SAFAHTRGISLVLRLLPVQCPRLETLGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVGHLNQHLDGHILTLEDPVE
FIHHSERCLIQQREIGRHCPSFSAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVEILIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPAVANLIREGKVHQLPGVMQTGMQAGMLTFTQSFQQR
VAAGQL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=719580 NWT75_RS03645 WP_258336473.1 737625..738605(+) (pilT) [Klebsiella quasipneumoniae strain KA1]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATTCCGCCGCACCGCG
CTGGCGGCGGCAGGGCAAGCTGGAGTCCGCTCCCTTTCCCGCGCCGGATATCGCGGATTTACTCAACGACTGGCTCGATG
CCGCACAGTTCCAGCACTGGCAGGAGCACGGCCAGATTGACTTTGCGCTGACTCTGGCCTGCGGTTCGCGGCTACGCGCC
AGCGCTTTCGCCCATACGCGCGGAATATCGCTGGTGCTGCGCCTGCTGCCCGTCCAGTGCCCGCGTCTGGAGACGCTGGG
CGCGCCGCCTGCGCTAAGCGAACTACTGGCGGAAGAGAGTGGCTTGCTGCTGGTCACCGGGGCGACGGGCAGCGGCAAGT
CGACTACCCTGGCGGCGATGGTCGGGCATCTCAACCAGCATCTTGATGGCCATATTCTGACGCTGGAGGATCCGGTGGAG
TTTATTCATCACAGCGAGCGCTGCCTGATCCAGCAGCGGGAGATTGGCCGTCACTGTCCTTCGTTCTCCGCGGCGCTTCG
GGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGCTTGGGGAATTACGCGACAGCGAAACCATCCGCCTGGCGTTGACGG
CTGCGGAAACCGGGCATCTTGTGATGGCGACATTACACACCCGCGGCGCGGCGCCGGCGGTGGAGATATTGATCGATGTC
TTTCCGGCAGAGGAGAAAGATCAGGTTCGTAGTCAGCTGGCCGGAAGCCTGTGCGCGGTGCTGGCGCAAAAATTATTACC
CGCACGTCAGGGCGGCAGAGTTGCGCTATACGAGCTTCTGGTCAATACGCCGGCGGTGGCGAATTTGATCCGCGAAGGAA
AAGTGCACCAGTTGCCCGGTGTTATGCAGACCGGGATGCAGGCCGGAATGCTGACATTTACCCAGAGTTTTCAGCAACGT
GTGGCCGCAGGCCAGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.376

100

0.515
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.376

100

0.515
  pilT Acinetobacter baylyi ADP1

48.318

100

0.485
  pilT Acinetobacter baumannii strain A118

47.706

100

0.479
  pilT Acinetobacter baumannii D1279779

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

47.401

100

0.475
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Pseudomonas stutzeri DSM 10701

47.095

100

0.472
  pilT Legionella pneumophila strain ERS1305867

47.531

99.387

0.472
  pilT Legionella pneumophila strain Lp02

47.531

99.387

0.472
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.849

97.239

0.426
  pilU Vibrio cholerae strain A1552

40.122

100

0.405
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Acinetobacter baylyi ADP1

38.941

98.466

0.383