Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PT256_RS09860 Genome accession   NZ_CP117817
Coordinates   2157511..2158545 (-) Length   344 a.a.
NCBI ID   WP_027220544.1    Uniprot ID   -
Organism   Legionella pneumophila strain D5573     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2152511..2163545
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT256_RS09840 (PT256_09840) rpoZ 2154419..2154622 (-) 204 WP_011214258.1 DNA-directed RNA polymerase subunit omega -
  PT256_RS09845 (PT256_09845) gmk 2154673..2155302 (-) 630 WP_027220542.1 guanylate kinase -
  PT256_RS09850 (PT256_09850) - 2155308..2156174 (-) 867 WP_027220543.1 YicC/YloC family endoribonuclease -
  PT256_RS09855 (PT256_09855) rph 2156476..2157183 (+) 708 WP_010947728.1 ribonuclease PH -
  PT256_RS09860 (PT256_09860) pilT 2157511..2158545 (-) 1035 WP_027220544.1 type IV pilus twitching motility protein PilT Machinery gene
  PT256_RS09865 (PT256_09865) - 2158709..2159401 (+) 693 WP_027225477.1 YggS family pyridoxal phosphate-dependent enzyme -
  PT256_RS09870 (PT256_09870) proC 2159401..2160189 (+) 789 WP_027220546.1 pyrroline-5-carboxylate reductase -
  PT256_RS09875 (PT256_09875) - 2160305..2160568 (-) 264 WP_061784318.1 GIY-YIG nuclease family protein -
  PT256_RS09880 (PT256_09880) - 2160571..2160939 (-) 369 Protein_1883 IS4 family transposase -
  PT256_RS09885 (PT256_09885) - 2161399..2161974 (+) 576 WP_061784319.1 YggT family protein -
  PT256_RS09890 (PT256_09890) - 2162034..2162636 (+) 603 WP_014842061.1 DUF2845 domain-containing protein -
  PT256_RS09895 (PT256_09895) - 2162650..2162931 (+) 282 WP_010947734.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38352.25 Da        Isoelectric Point: 7.0290

>NTDB_id=719331 PT256_RS09860 WP_027220544.1 2157511..2158545(-) (pilT) [Legionella pneumophila strain D5573]
MDIAELLAFSVKNKSSDLHISAGMPPMIRVDGDLRKINLPPLEHKDVIKIIYDIMNDRQRKEYEEFLETDFSFEIANLAR
FRVNAFNQSRGAAAVFRTIPSEILSMEDLGLPPIFKEMASFSRGLVLVTGPTGSGKSTTLAAVIDYINSSRYEHILTVED
PIEFVHQSKKCLVNQREVHKDTLSFNAALRSALREDPDIILVGELRDLETIRLAMTAAETGHLVFGTLHTNSATKTINRI
IDVFPAEEKSMVRSMLSESLQAVVAQSLLKKNKGGRVAALEIMMCTGAIRNLIREDKIAQMYSSIQTGQAKGMQTLDQHL
TELVNKNIISKHTAHEAALNKSLF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=719331 PT256_RS09860 WP_027220544.1 2157511..2158545(-) (pilT) [Legionella pneumophila strain D5573]
ATGGATATCGCGGAATTGTTGGCATTTTCAGTTAAAAACAAGTCATCAGATTTGCATATTTCAGCTGGCATGCCTCCTAT
GATTCGTGTTGATGGTGATTTACGCAAAATTAATTTACCTCCATTAGAGCATAAAGACGTCATAAAGATTATTTATGACA
TAATGAATGACAGACAAAGAAAAGAATATGAAGAATTTCTTGAAACAGATTTTTCCTTTGAAATAGCCAATCTGGCACGG
TTCAGGGTTAATGCTTTCAATCAATCACGCGGTGCCGCTGCTGTTTTCAGAACTATTCCCTCTGAAATTCTTAGCATGGA
AGATTTAGGATTACCCCCAATTTTTAAAGAAATGGCCAGTTTTTCCAGAGGGCTGGTTCTGGTTACTGGCCCAACTGGAT
CAGGAAAAAGTACCACGCTTGCAGCAGTCATCGATTATATTAATTCAAGTCGTTATGAGCATATTTTGACAGTTGAAGAT
CCCATTGAGTTTGTTCATCAAAGCAAAAAATGTCTGGTCAATCAACGTGAAGTGCACAAAGACACATTGAGCTTTAATGC
CGCTTTGAGATCAGCGCTACGTGAAGATCCTGATATTATATTGGTGGGTGAATTACGTGATTTGGAAACTATACGCTTGG
CAATGACCGCAGCAGAAACCGGGCATTTGGTTTTTGGAACATTGCATACCAATTCTGCAACAAAAACAATAAACAGGATA
ATTGATGTATTTCCTGCTGAAGAAAAATCGATGGTACGCTCTATGCTTTCTGAATCATTACAAGCTGTCGTTGCTCAAAG
TTTATTAAAAAAGAATAAAGGTGGTCGGGTTGCGGCGCTTGAAATTATGATGTGTACCGGGGCTATTCGTAATTTAATTC
GTGAGGATAAGATAGCGCAAATGTATTCGTCAATTCAAACTGGTCAAGCCAAAGGAATGCAAACATTGGATCAACATTTG
ACTGAATTGGTTAATAAAAATATTATTTCAAAACACACTGCTCATGAGGCTGCCCTGAACAAGTCATTATTTTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

99.709

100

0.997
  pilT Legionella pneumophila strain Lp02

99.709

100

0.997
  pilT Acinetobacter baumannii D1279779

75.872

100

0.759
  pilT Acinetobacter baumannii strain A118

75.872

100

0.759
  pilT Acinetobacter nosocomialis M2

75.872

100

0.759
  pilT Acinetobacter baylyi ADP1

73.547

100

0.735
  pilT Pseudomonas aeruginosa PAK

74.852

98.256

0.735
  pilT Pseudomonas stutzeri DSM 10701

74.556

98.256

0.733
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.781

95.64

0.648
  pilT Vibrio cholerae strain A1552

67.781

95.64

0.648
  pilT Neisseria meningitidis 8013

65.103

99.128

0.645
  pilT Neisseria gonorrhoeae MS11

64.809

99.128

0.642
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.494
  pilU Vibrio cholerae strain A1552

42.773

98.547

0.422
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Acinetobacter baylyi ADP1

40.361

96.512

0.39