Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NV349_RS05815 Genome accession   NZ_CP102798
Coordinates   1245813..1246859 (+) Length   348 a.a.
NCBI ID   WP_036128104.1    Uniprot ID   A0A0W7YJX6
Organism   Lysinibacillus sp. OF-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1240813..1251859
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NV349_RS05795 (NV349_05795) - 1241561..1242019 (-) 459 WP_180957770.1 type II secretion system protein -
  NV349_RS05800 (NV349_05800) - 1242024..1242470 (-) 447 WP_058843953.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NV349_RS05805 (NV349_05805) - 1242782..1244113 (+) 1332 WP_271912557.1 G5 domain-containing protein -
  NV349_RS05810 (NV349_05810) - 1244131..1245801 (+) 1671 WP_231745020.1 ATPase, T2SS/T4P/T4SS family -
  NV349_RS05815 (NV349_05815) pilT 1245813..1246859 (+) 1047 WP_036128104.1 type IV pilus twitching motility protein PilT Machinery gene
  NV349_RS05820 (NV349_05820) - 1246856..1248061 (+) 1206 WP_058843955.1 type II secretion system F family protein -
  NV349_RS05825 (NV349_05825) - 1248149..1248634 (+) 486 WP_036128097.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NV349_RS05830 (NV349_05830) pilM 1248747..1249721 (+) 975 WP_271912558.1 pilus assembly protein PilM -
  NV349_RS05835 (NV349_05835) - 1249721..1250314 (+) 594 WP_058843957.1 PilN domain-containing protein -
  NV349_RS05840 (NV349_05840) - 1250311..1251000 (+) 690 WP_058843958.1 hypothetical protein -
  NV349_RS05845 (NV349_05845) - 1251068..1251820 (+) 753 WP_089932056.1 A24 family peptidase -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38694.43 Da        Isoelectric Point: 7.0193

>NTDB_id=719072 NV349_RS05815 WP_036128104.1 1245813..1246859(+) (pilT) [Lysinibacillus sp. OF-1]
MTSYSIVELLTRAFEEKASDLHLTKGIPPVYRIHGQLEHYGADPLDSEELKGMVNAILPVHKQQEFEGKGETDFNFSLPG
KCRFRVNAYHQRNEVTIAARLIEAKIPSIEALNMPQVLYKLAEKPQGLILVTGPTGSGKSTTLAAMIDYINETRSKHIIT
LEDPIEYLHTHKKSIINQREVGVDTMSFANGLRASLRQDPDIILVGEMRDYETISTAITAAETGHLVMATLHTSSAPTTI
DRIIDVFPPHQQSQIRVQLANVLQGIVSQRLFIRKDVKGRIAATEILVQAPAISNLIRNEKIHQIPSVMQTSKALGMHTL
ESSMQHLITTGKISLEDARPYMNAGDYS

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=719072 NV349_RS05815 WP_036128104.1 1245813..1246859(+) (pilT) [Lysinibacillus sp. OF-1]
ATGACATCTTATTCAATCGTTGAATTATTAACACGTGCGTTTGAAGAAAAAGCATCGGATCTTCATTTAACAAAAGGTAT
TCCGCCAGTATATCGTATTCATGGGCAGTTGGAACATTATGGTGCAGATCCATTAGATTCTGAAGAATTAAAGGGAATGG
TAAATGCCATTCTCCCTGTACATAAACAACAGGAGTTTGAGGGAAAGGGAGAAACTGACTTTAACTTTTCATTGCCTGGG
AAATGTCGATTCCGTGTAAATGCCTATCATCAGCGAAATGAAGTGACAATAGCGGCTCGTTTAATTGAAGCTAAAATTCC
ATCTATTGAAGCACTTAATATGCCACAAGTGTTATATAAGCTTGCGGAAAAACCACAAGGTTTAATTTTAGTAACTGGTC
CAACAGGCTCAGGTAAATCAACTACATTAGCAGCAATGATTGATTATATCAACGAGACAAGATCAAAACATATTATTACG
TTAGAGGATCCAATTGAATACTTGCATACTCATAAAAAGTCTATCATCAATCAGCGTGAGGTTGGTGTCGATACAATGAG
CTTTGCGAATGGTTTACGAGCATCATTGCGTCAAGACCCAGATATTATTTTAGTTGGGGAGATGCGAGATTATGAAACGA
TTTCAACGGCTATCACGGCTGCTGAAACAGGGCATTTAGTAATGGCAACACTTCATACAAGTAGTGCACCAACTACAATC
GACCGTATTATTGATGTATTTCCACCGCATCAGCAAAGTCAAATTCGAGTGCAGCTTGCGAATGTACTGCAAGGTATTGT
TTCACAGCGTTTATTTATTCGTAAAGATGTAAAAGGGCGCATTGCTGCGACAGAAATTTTAGTACAAGCTCCTGCCATTT
CGAATTTAATTCGCAATGAAAAAATTCACCAAATTCCAAGCGTAATGCAGACAAGTAAAGCATTGGGCATGCACACATTA
GAATCTTCGATGCAGCACCTGATTACCACAGGTAAGATCTCGCTAGAAGATGCACGGCCATACATGAACGCTGGTGATTA
TAGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W7YJX6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.663

97.126

0.511
  pilT Neisseria meningitidis 8013

50.599

95.977

0.486
  pilT Neisseria gonorrhoeae MS11

50.299

95.977

0.483
  pilT Pseudomonas aeruginosa PAK

49.701

95.977

0.477
  pilT Pseudomonas stutzeri DSM 10701

49.401

95.977

0.474
  pilT Acinetobacter baumannii strain A118

49.401

95.977

0.474
  pilT Acinetobacter baumannii D1279779

49.401

95.977

0.474
  pilT Acinetobacter nosocomialis M2

49.401

95.977

0.474
  pilT Acinetobacter baylyi ADP1

49.102

95.977

0.471
  pilT Vibrio cholerae strain A1552

48.802

95.977

0.468
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.802

95.977

0.468
  pilT Legionella pneumophila strain Lp02

49.24

94.54

0.466
  pilT Legionella pneumophila strain ERS1305867

49.24

94.54

0.466
  pilU Pseudomonas stutzeri DSM 10701

43.202

95.115

0.411
  pilU Vibrio cholerae strain A1552

43.168

92.529

0.399
  pilU Acinetobacter baylyi ADP1

39.941

97.126

0.388