Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NVS55_RS31265 Genome accession   NZ_CP102770
Coordinates   8009983..8011095 (-) Length   370 a.a.
NCBI ID   WP_342375764.1    Uniprot ID   -
Organism   Myxococcus stipitatus strain YRE12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 8004983..8016095
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NVS55_RS31250 (NVS55_31340) - 8005636..8007024 (-) 1389 WP_342382067.1 sigma-54 dependent transcriptional regulator -
  NVS55_RS31255 (NVS55_31345) - 8007072..8008625 (-) 1554 WP_342375762.1 ATP-binding protein -
  NVS55_RS31260 (NVS55_31350) - 8008672..8009925 (-) 1254 WP_342375763.1 type II secretion system F family protein -
  NVS55_RS31265 (NVS55_31355) pilT 8009983..8011095 (-) 1113 WP_342375764.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NVS55_RS31270 (NVS55_31360) pilB 8011134..8012834 (-) 1701 WP_342375765.1 type IV-A pilus assembly ATPase PilB -
  NVS55_RS31275 (NVS55_31365) - 8013050..8013979 (-) 930 WP_342375766.1 bifunctional riboflavin kinase/FAD synthetase -
  NVS55_RS31280 (NVS55_31370) - 8014049..8014669 (+) 621 WP_342375767.1 tRNA (guanine-N7)-methyltransferase -
  NVS55_RS31285 (NVS55_31375) - 8014778..8015821 (+) 1044 WP_342375768.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40502.83 Da        Isoelectric Point: 8.2933

>NTDB_id=718971 NVS55_RS31265 WP_342375764.1 8009983..8011095(-) (pilT) [Myxococcus stipitatus strain YRE12]
MANLHQLLKAMVEKGASDLHVTTGSPPQLRVDGELVPLKTAPLTPVETKQLCYSILTDAQKHKFEEDNELDLSFGVKGLS
RFRANIFMQRGAVAGAFRTIPFKILTFQELGLPPVVAELVKKPRGLILVTGPTGSGKSTTLASMIDKINTERHEHIMTIE
DPIEYLHPHKNCLVNQREVGADTRNFKTALKYILRQDPDVVLVGELRDLETIEAALTIAETGHICYATLHTNSAVQTINR
VLDVFPPYQQPQVRAQLSFVLEGVMSQALVAKAGAPGRVLALEVMVPNPAIRNLIREDKVHQIYSSMQVGQAKFGMQTFN
QALAALLLRRVITQDEAFGRSSDPDELRNILATGGGMPVGQRAPGGPAGR

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=718971 NVS55_RS31265 WP_342375764.1 8009983..8011095(-) (pilT) [Myxococcus stipitatus strain YRE12]
GTGGCCAACCTGCACCAGCTCCTCAAGGCGATGGTCGAGAAGGGCGCTTCCGACCTCCACGTCACCACCGGCTCTCCGCC
CCAGTTGCGCGTGGACGGCGAGCTCGTCCCCTTGAAGACGGCGCCGCTGACGCCCGTGGAGACCAAGCAGCTCTGCTACT
CCATCCTCACGGACGCCCAGAAGCACAAGTTCGAGGAGGACAATGAGCTGGACCTGTCCTTCGGCGTGAAGGGCCTGTCG
CGCTTCCGCGCCAACATCTTCATGCAGCGCGGCGCGGTGGCCGGGGCCTTCCGTACCATTCCCTTCAAGATCCTGACGTT
CCAGGAGCTGGGCCTGCCGCCCGTGGTCGCGGAGCTGGTGAAGAAGCCGCGCGGCCTCATCCTGGTGACGGGCCCCACGG
GCTCGGGCAAGTCCACCACGCTGGCCTCGATGATCGACAAGATCAACACCGAGCGTCATGAGCACATCATGACCATCGAG
GACCCCATCGAGTACCTGCACCCACACAAGAACTGCCTGGTGAACCAGCGCGAGGTGGGAGCGGACACCCGGAACTTCAA
GACGGCCCTCAAGTACATCCTCCGCCAGGACCCGGACGTGGTGCTGGTCGGTGAGCTCCGAGACCTCGAGACCATCGAGG
CGGCCCTCACCATCGCGGAGACGGGCCATATCTGCTACGCGACACTCCACACCAACAGCGCGGTGCAGACCATCAACCGC
GTGCTGGACGTGTTCCCGCCGTACCAGCAGCCGCAGGTGCGCGCGCAGCTCTCCTTCGTGCTGGAGGGCGTGATGAGTCA
GGCGCTGGTGGCCAAGGCGGGTGCTCCGGGCCGCGTGCTGGCGCTGGAGGTCATGGTGCCCAACCCCGCCATCCGGAACC
TCATCCGCGAGGACAAGGTCCACCAGATCTACTCCTCGATGCAGGTGGGTCAGGCGAAGTTCGGCATGCAGACGTTCAAC
CAGGCGCTGGCCGCGCTGCTCCTGCGGCGGGTCATCACCCAGGACGAGGCCTTCGGACGCTCCAGCGACCCGGACGAGCT
GCGCAACATCCTGGCGACTGGAGGTGGAATGCCGGTCGGACAGCGGGCTCCAGGCGGGCCGGCGGGTCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.811

100

0.508
  pilT Legionella pneumophila strain ERS1305867

52.836

90.541

0.478
  pilT Legionella pneumophila strain Lp02

52.836

90.541

0.478
  pilT Acinetobacter baumannii D1279779

50.725

93.243

0.473
  pilT Acinetobacter baumannii strain A118

50.725

93.243

0.473
  pilT Acinetobacter nosocomialis M2

50.725

93.243

0.473
  pilT Pseudomonas stutzeri DSM 10701

50.725

93.243

0.473
  pilT Pseudomonas aeruginosa PAK

50.725

93.243

0.473
  pilT Acinetobacter baylyi ADP1

50.435

93.243

0.47
  pilT Vibrio cholerae strain A1552

48.802

90.27

0.441
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.802

90.27

0.441
  pilT Neisseria meningitidis 8013

44.282

92.162

0.408
  pilT Neisseria gonorrhoeae MS11

44.282

92.162

0.408
  pilU Vibrio cholerae strain A1552

42.297

96.486

0.408
  pilU Pseudomonas stutzeri DSM 10701

41.026

94.865

0.389
  pilU Acinetobacter baylyi ADP1

39.769

93.784

0.373