Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NR302_RS15655 Genome accession   NZ_CP102604
Coordinates   3396703..3397695 (-) Length   330 a.a.
NCBI ID   WP_258143891.1    Uniprot ID   -
Organism   Pantoea sp. SOD02     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3391703..3402695
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NR302_RS15625 (NR302_15625) - 3392395..3392907 (+) 513 WP_258143889.1 SprT family zinc-dependent metalloprotease -
  NR302_RS15630 (NR302_15630) endA 3393021..3393728 (+) 708 WP_258143890.1 deoxyribonuclease I -
  NR302_RS15635 (NR302_15635) rsmE 3393803..3394534 (+) 732 WP_008107252.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NR302_RS15640 (NR302_15640) gshB 3394612..3395556 (+) 945 WP_008107250.1 glutathione synthase -
  NR302_RS15645 (NR302_15645) - 3395639..3396202 (+) 564 WP_008107249.1 YqgE/AlgH family protein -
  NR302_RS15650 (NR302_15650) ruvX 3396202..3396618 (+) 417 WP_061720236.1 Holliday junction resolvase RuvX -
  NR302_RS15655 (NR302_15655) pilT 3396703..3397695 (-) 993 WP_258143891.1 type IV pilus twitching motility protein PilT Machinery gene
  NR302_RS15660 (NR302_15660) - 3397712..3398419 (+) 708 WP_258143892.1 YggS family pyridoxal phosphate-dependent enzyme -
  NR302_RS15665 (NR302_15665) - 3398432..3398986 (+) 555 WP_008107240.1 YggT family protein -
  NR302_RS15670 (NR302_15670) - 3399007..3399600 (+) 594 WP_154155286.1 XTP/dITP diphosphatase -
  NR302_RS15675 (NR302_15675) hemW 3399593..3400747 (+) 1155 WP_258143893.1 radical SAM family heme chaperone HemW -
  NR302_RS15680 (NR302_15680) - 3400783..3401499 (-) 717 WP_258143894.1 DUF2884 domain-containing protein -
  NR302_RS15685 (NR302_15685) - 3401602..3401928 (-) 327 WP_008107231.1 YggL family protein -
  NR302_RS15690 (NR302_15690) trmB 3401928..3402647 (-) 720 WP_258143895.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 35816.19 Da        Isoelectric Point: 6.6166

>NTDB_id=718094 NR302_RS15655 WP_258143891.1 3396703..3397695(-) (pilT) [Pantoea sp. SOD02]
MELDEIVALSVKHNAADLHLCSGHLPHWRRQGVLEPIPQQNALDGGWLEAFMQQWLTASQQAELEENGHVDFAITLASGI
RLRANLFMQRHGLSLALRLIASQAPDLSSLHLPDVVSQLLQLEEGLILITGATGSGKSTTLAAMVDCLNRQQARHILTLE
DPIEFVHRSQRSLIQQREVGAHCASFQHGLKAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGATQAVDR
LVDVFPAEEKNLVRTQLAGSLKAVLAQRLVPAKAGGRIGLFEVLVATPAVANLIREGKMHQLPGVLQTGAQAGMQTFAQS
EQARQLAGMI

Nucleotide


Download         Length: 993 bp        

>NTDB_id=718094 NR302_RS15655 WP_258143891.1 3396703..3397695(-) (pilT) [Pantoea sp. SOD02]
ATGGAGTTGGATGAAATTGTGGCCCTTAGTGTAAAGCATAACGCCGCCGATCTGCACCTTTGCAGCGGACATTTGCCGCA
CTGGCGGCGACAAGGCGTGCTGGAGCCGATTCCGCAGCAAAACGCACTGGATGGCGGCTGGCTGGAAGCCTTTATGCAGC
AGTGGCTCACAGCGTCGCAACAGGCCGAGCTGGAGGAGAACGGCCACGTTGACTTTGCCATCACGCTTGCCAGCGGCATT
CGACTGCGCGCCAACCTTTTTATGCAGCGTCACGGCTTATCGCTGGCGCTGCGGTTGATTGCCAGCCAGGCGCCCGATCT
CAGCAGTCTGCATTTACCCGACGTAGTGAGCCAGCTGTTACAGCTGGAAGAGGGGCTGATTTTAATTACCGGCGCCACCG
GCAGCGGCAAATCCACTACGCTGGCGGCGATGGTGGATTGCCTGAACCGCCAGCAGGCGCGGCACATCCTGACGCTGGAA
GATCCGATTGAGTTTGTGCATCGCAGCCAGCGATCGCTGATTCAGCAGCGCGAAGTCGGCGCACATTGCGCATCGTTTCA
ACACGGGCTGAAAGCCGCGCTGCGTGAAGATCCTGATGTGATTCTGCTCGGCGAACTGCGCGACAGCGAAACCATCCGCC
TGGCGCTCACTGCGGCAGAAACCGGCCATCTGGTGCTGGCTACGCTCCATACGCGCGGCGCAACCCAGGCGGTTGATAGG
CTGGTGGATGTGTTCCCTGCCGAGGAGAAAAACCTGGTGCGCACCCAATTAGCGGGCAGCCTGAAAGCGGTGCTGGCGCA
GCGTTTAGTGCCGGCGAAAGCGGGCGGGCGTATTGGCTTGTTTGAAGTGTTGGTGGCGACGCCGGCGGTGGCGAATCTGA
TTCGCGAGGGCAAGATGCATCAGTTGCCGGGCGTGTTGCAAACCGGTGCGCAGGCGGGCATGCAGACATTTGCGCAGAGC
GAGCAGGCGCGGCAGTTAGCGGGGATGATTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(48-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

48.048

100

0.485
  pilT Neisseria gonorrhoeae MS11

48.048

100

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.78

99.394

0.485
  pilT Vibrio cholerae strain A1552

48.78

99.394

0.485
  pilT Acinetobacter baylyi ADP1

47.188

96.97

0.458
  pilT Pseudomonas stutzeri DSM 10701

47.319

96.061

0.455
  pilT Legionella pneumophila strain ERS1305867

46.708

96.667

0.452
  pilT Legionella pneumophila strain Lp02

46.708

96.667

0.452
  pilT Acinetobacter nosocomialis M2

46.25

96.97

0.448
  pilT Acinetobacter baumannii D1279779

46.25

96.97

0.448
  pilT Acinetobacter baumannii strain A118

46.25

96.97

0.448
  pilT Pseudomonas aeruginosa PAK

46.372

96.061

0.445
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.41

97.576

0.433
  pilU Pseudomonas stutzeri DSM 10701

41.925

97.576

0.409
  pilU Vibrio cholerae strain A1552

41.121

97.273

0.4
  pilU Acinetobacter baylyi ADP1

37.578

97.576

0.367