Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NQZ71_RS07510 Genome accession   NZ_CP102589
Coordinates   1546872..1547924 (+) Length   350 a.a.
NCBI ID   WP_317011601.1    Uniprot ID   -
Organism   Niallia taxi strain NBC_Nia     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1541872..1552924
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQZ71_RS07495 (NQZ71_07515) - 1543004..1543780 (+) 777 WP_144456413.1 PRC-barrel domain-containing protein -
  NQZ71_RS07500 (NQZ71_07520) - 1543802..1545178 (+) 1377 WP_317011599.1 G5 domain-containing protein -
  NQZ71_RS07505 (NQZ71_07525) - 1545199..1546860 (+) 1662 WP_260053761.1 GspE/PulE family protein -
  NQZ71_RS07510 (NQZ71_07530) pilT 1546872..1547924 (+) 1053 WP_317011601.1 type IV pilus twitching motility protein PilT Machinery gene
  NQZ71_RS07515 (NQZ71_07535) - 1547911..1549116 (+) 1206 WP_317011602.1 type II secretion system F family protein -
  NQZ71_RS07520 (NQZ71_07540) - 1549233..1549661 (+) 429 WP_317011603.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NQZ71_RS07525 (NQZ71_07545) - 1549692..1550438 (+) 747 WP_317011604.1 prepilin peptidase -
  NQZ71_RS07530 (NQZ71_07550) pilM 1550450..1551430 (+) 981 WP_144456399.1 type IV pilus biogenesis protein PilM -
  NQZ71_RS07535 (NQZ71_07555) - 1551433..1552068 (+) 636 WP_144456397.1 fimbrial assembly protein -
  NQZ71_RS07540 (NQZ71_07560) - 1552065..1552826 (+) 762 WP_260053766.1 pilus assembly protein PilO -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38779.66 Da        Isoelectric Point: 6.9765

>NTDB_id=717929 NQZ71_RS07510 WP_317011601.1 1546872..1547924(+) (pilT) [Niallia taxi strain NBC_Nia]
MYNNIDHLLRVGFELKASDIHLTVGVPPVMRINGELRRYGKESLTPADTEKMAKEMIPEDLWDAFKEKGELDFSYSLTGV
SRFRVNTYKQRACVALAIRVVPTSIPNLDDLQLPQILKKISEKPQGLILVTGPTGSGKSTTLAAMIAYMNDTMRKHIITL
EDPIEYLHKHGKCIIDQREVGFDTNNFANGLRASLRQDPDVILVGEMRDLETIQTAITAAETGHLVLGTLHTSSAPATIN
RIIDVFPPSQQDQIRIQLASVLVSIVSQRLFPTPDKSGRVSATEILINNAAVANLIRNEKIHQILNIMQTSRAAGMHTLE
SNVKELVQKGAILREAALPYLQEEQSNGSL

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=717929 NQZ71_RS07510 WP_317011601.1 1546872..1547924(+) (pilT) [Niallia taxi strain NBC_Nia]
GTGTATAACAATATCGACCATTTGCTTCGGGTCGGCTTCGAGCTGAAAGCTTCAGACATCCATTTGACGGTCGGCGTTCC
TCCAGTCATGCGGATAAACGGAGAGCTAAGAAGATATGGTAAAGAAAGCCTAACCCCTGCAGATACAGAGAAAATGGCAA
AAGAGATGATTCCAGAGGATTTATGGGATGCGTTTAAGGAAAAAGGAGAGCTTGATTTTTCCTATAGCTTAACGGGCGTT
TCCAGATTTCGTGTCAACACATATAAGCAGAGAGCCTGTGTTGCTCTGGCGATTAGGGTTGTGCCGACATCAATTCCGAA
CTTGGATGATTTGCAGCTGCCGCAAATTTTGAAGAAAATCAGCGAAAAGCCGCAAGGACTCATATTAGTAACAGGACCGA
CAGGAAGCGGTAAATCAACAACACTTGCAGCGATGATTGCTTATATGAACGATACAATGAGAAAGCATATTATCACATTA
GAAGACCCAATTGAGTATTTGCATAAGCATGGCAAATGCATCATTGACCAGCGAGAAGTCGGGTTTGACACAAACAACTT
TGCAAATGGTTTGCGCGCATCCTTAAGACAGGATCCGGACGTTATTCTTGTTGGTGAGATGCGTGACTTAGAGACCATTC
AAACGGCAATAACAGCAGCAGAAACAGGCCATTTAGTGCTTGGTACTCTTCATACATCAAGTGCTCCGGCAACAATTAAC
CGTATTATTGATGTATTTCCGCCATCCCAGCAGGATCAAATTCGTATCCAGCTAGCATCTGTTCTTGTCAGTATTGTGTC
ACAACGGCTGTTTCCAACACCTGATAAATCAGGTAGGGTTTCTGCAACAGAAATTCTCATTAATAATGCAGCTGTCGCCA
ACTTGATTCGCAATGAAAAAATTCACCAAATCTTGAATATCATGCAGACATCAAGAGCCGCGGGCATGCACACATTAGAA
AGCAATGTGAAGGAGCTTGTCCAAAAAGGTGCGATATTAAGAGAAGCAGCACTGCCATATTTACAGGAGGAGCAAAGCAA
TGGCTCGTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(94-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.448

95.714

0.483
  pilT Vibrio cholerae strain A1552

50.606

94.286

0.477
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.606

94.286

0.477
  pilT Neisseria meningitidis 8013

47.076

97.714

0.46
  pilT Neisseria gonorrhoeae MS11

47.076

97.714

0.46
  pilT Acinetobacter nosocomialis M2

46.064

98

0.451
  pilT Acinetobacter baumannii D1279779

46.064

98

0.451
  pilT Acinetobacter baumannii strain A118

46.064

98

0.451
  pilT Legionella pneumophila strain Lp02

46.707

95.429

0.446
  pilT Legionella pneumophila strain ERS1305867

46.707

95.429

0.446
  pilT Pseudomonas aeruginosa PAK

46.407

95.429

0.443
  pilT Acinetobacter baylyi ADP1

46.505

94

0.437
  pilT Pseudomonas stutzeri DSM 10701

45.808

95.429

0.437
  pilU Pseudomonas stutzeri DSM 10701

42.566

98

0.417
  pilU Acinetobacter baylyi ADP1

40.058

99.143

0.397
  pilU Vibrio cholerae strain A1552

40.248

92.286

0.371