Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NR798_RS37225 Genome accession   NZ_CP102578
Coordinates   9460024..9461136 (-) Length   370 a.a.
NCBI ID   WP_375767302.1    Uniprot ID   -
Organism   Archangium gephyra strain SDU49     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 9455024..9466136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NR798_RS37210 (NR798_37325) - 9455681..9457075 (-) 1395 WP_375773886.1 sigma-54-dependent transcriptional regulator -
  NR798_RS37215 (NR798_37330) - 9457098..9458588 (-) 1491 WP_375773887.1 nitrogen regulation protein NR(II) -
  NR798_RS37220 (NR798_37335) - 9458716..9459972 (-) 1257 WP_375767301.1 type II secretion system F family protein -
  NR798_RS37225 (NR798_37340) pilT 9460024..9461136 (-) 1113 WP_375767302.1 type IV pilus twitching motility protein PilT Machinery gene
  NR798_RS37230 (NR798_37345) pilB 9461184..9462887 (-) 1704 WP_375767303.1 type IV-A pilus assembly ATPase PilB -
  NR798_RS37235 (NR798_37350) - 9463078..9464016 (-) 939 WP_375767304.1 bifunctional riboflavin kinase/FAD synthetase -
  NR798_RS37240 (NR798_37355) - 9464108..9464728 (+) 621 WP_375767305.1 tRNA (guanine(46)-N(7))-methyltransferase TrmB -
  NR798_RS37245 (NR798_37360) - 9464829..9465884 (+) 1056 WP_375767306.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40582.75 Da        Isoelectric Point: 8.2886

>NTDB_id=717769 NR798_RS37225 WP_375767302.1 9460024..9461136(-) (pilT) [Archangium gephyra strain SDU49]
MNLHQLLKAMVEKGASDLHITTGSPPQLRVDGELVPLKTAQLTPVETKQLCYSILTDAQKHKFEEENELDLSFGVKGLSR
FRANIYMQRGAVAGAFRTIPFKILTFQELGLPPVVAELVKRPRGLILVTGPTGSGKSTTLASMVDKINTDRHEHIMTIED
PIEYLHPHKNCLVNQREVGADTRNFKTALKYILRQDPDVVLVGELRDLETIEAALVIAETGHTCYATLHTNSAVQTINRV
LDVFPPYQQPQVRAQLSFVLEGVMSQSLIAKSGSPGRVLALEVMIPNPAIRNLIREDKVHQVYSAMQVGQAKFGMQTFNQ
ALAQLLARRLISQEEAFGRSSDPDELRNILSAGAGPGGMAGQRPAGPGGR

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=717769 NR798_RS37225 WP_375767302.1 9460024..9461136(-) (pilT) [Archangium gephyra strain SDU49]
GTGAATCTGCACCAGCTGCTCAAGGCGATGGTCGAGAAGGGCGCTTCCGACCTCCACATCACCACCGGCTCCCCGCCCCA
GCTCCGCGTGGACGGCGAGCTGGTGCCGCTCAAGACGGCCCAGCTCACCCCGGTGGAGACCAAGCAGCTCTGCTACTCCA
TCCTCACCGACGCCCAGAAGCACAAGTTCGAGGAGGAGAACGAGCTCGACCTGTCCTTCGGTGTGAAGGGGCTCTCCCGC
TTCCGCGCCAACATCTACATGCAGCGCGGCGCGGTGGCCGGGGCCTTCCGTACCATTCCCTTCAAGATCCTCACCTTCCA
GGAGCTGGGTCTTCCCCCGGTGGTGGCCGAGCTGGTGAAGCGGCCGCGCGGCCTCATCCTGGTGACGGGTCCCACGGGCT
CGGGCAAGTCCACCACGCTCGCGTCCATGGTGGACAAGATCAACACCGATCGTCACGAGCACATCATGACGATCGAGGAT
CCGATCGAGTACCTGCACCCGCACAAGAACTGCCTGGTGAACCAGCGCGAGGTGGGTGCGGACACGCGCAACTTCAAGAC
GGCGCTCAAGTACATCCTCCGCCAGGACCCGGACGTGGTGCTGGTGGGCGAGCTGCGAGACCTGGAGACGATCGAAGCGG
CGCTCGTCATCGCCGAGACGGGCCACACCTGTTACGCCACGCTGCACACCAACAGCGCGGTGCAGACCATCAACCGTGTG
CTGGACGTGTTCCCGCCGTACCAGCAGCCGCAGGTGCGCGCGCAGCTGTCCTTCGTGCTGGAAGGCGTGATGAGCCAGTC
GCTCATCGCCAAGTCGGGCTCTCCGGGCCGCGTGCTGGCGCTGGAGGTGATGATCCCCAACCCCGCCATCCGCAACCTCA
TCCGCGAGGACAAGGTGCACCAAGTGTACTCGGCGATGCAGGTGGGCCAGGCCAAGTTCGGCATGCAGACCTTCAATCAG
GCCCTGGCGCAGCTGCTGGCGCGCCGGCTCATCTCCCAGGAAGAGGCGTTCGGCCGCTCGAGCGATCCGGACGAGCTGCG
CAACATCCTGTCGGCGGGGGCGGGCCCTGGTGGCATGGCTGGTCAGCGGCCGGCCGGGCCGGGCGGACGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.722

94.324

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.185

99.459

0.489
  pilT Legionella pneumophila strain Lp02

52.802

91.622

0.484
  pilT Legionella pneumophila strain ERS1305867

52.802

91.622

0.484
  pilT Acinetobacter baumannii strain A118

51.156

93.514

0.478
  pilT Acinetobacter baumannii D1279779

51.156

93.514

0.478
  pilT Acinetobacter nosocomialis M2

50.867

93.514

0.476
  pilT Pseudomonas stutzeri DSM 10701

50.867

93.514

0.476
  pilT Acinetobacter baylyi ADP1

50.578

93.514

0.473
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.955

90.541

0.443
  pilT Vibrio cholerae strain A1552

48.955

90.541

0.443
  pilU Vibrio cholerae strain A1552

42.105

97.568

0.411
  pilT Neisseria meningitidis 8013

43.642

93.514

0.408
  pilT Neisseria gonorrhoeae MS11

43.642

93.514

0.408
  pilU Pseudomonas stutzeri DSM 10701

40.223

96.757

0.389
  pilU Acinetobacter baylyi ADP1

40.23

94.054

0.378