Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NR792_RS18140 Genome accession   NZ_CP102577
Coordinates   4668359..4669471 (-) Length   370 a.a.
NCBI ID   WP_207048093.1    Uniprot ID   -
Organism   Corallococcus exercitus strain SDU142     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4663359..4674471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NR792_RS18125 (NR792_18165) - 4664018..4665460 (-) 1443 WP_375761270.1 sigma-54-dependent transcriptional regulator -
  NR792_RS18130 (NR792_18170) - 4665457..4666992 (-) 1536 WP_375761644.1 nitrogen regulation protein NR(II) -
  NR792_RS18135 (NR792_18175) - 4667054..4668304 (-) 1251 WP_375761271.1 type II secretion system F family protein -
  NR792_RS18140 (NR792_18180) pilT 4668359..4669471 (-) 1113 WP_207048093.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NR792_RS18145 (NR792_18185) pilB 4669520..4671220 (-) 1701 WP_375761272.1 type IV-A pilus assembly ATPase PilB -
  NR792_RS18150 (NR792_18190) - 4671442..4672368 (-) 927 WP_375761273.1 bifunctional riboflavin kinase/FAD synthetase -
  NR792_RS18155 (NR792_18195) - 4672450..4673070 (+) 621 WP_375761274.1 tRNA (guanine(46)-N(7))-methyltransferase TrmB -
  NR792_RS18160 (NR792_18200) - 4673245..4674288 (+) 1044 WP_120527970.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40420.62 Da        Isoelectric Point: 8.2875

>NTDB_id=717748 NR792_RS18140 WP_207048093.1 4668359..4669471(-) (pilT) [Corallococcus exercitus strain SDU142]
MANLHQLLKAMVEKGSSDLHITTGSPPQLRVDGELVPLKTAPLTPVETKQLCYSILTDAQKHKFEEENELDLSFGVKGLS
RFRANIFMQRGAVAGAFRTIPFKILTFQELGLPPVVAELVKKPRGLILVTGPTGSGKSTTLASMIDKINTERHEHIMTIE
DPIEYLHPHKNCLVNQREVGADTRNFKTALKYILRQDPDVVLVGELRDLETIEAALTIAETGHICYATLHTNSAVQTINR
ILDVFPPYQQPQVRAQLSFVLEGVMSQALVAKAGGPGRVLALEVMVPNPAIRNLIREDKVHQIYSSMQVGQAKYGMQTFN
QALAALLARRLISQDEAFGRSSDPEELRNILATGGAPGGAQRPAGGAGAR

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=717748 NR792_RS18140 WP_207048093.1 4668359..4669471(-) (pilT) [Corallococcus exercitus strain SDU142]
GTGGCCAACCTGCACCAGCTCCTCAAGGCGATGGTCGAGAAGGGCTCTTCCGACCTCCACATCACCACCGGCTCGCCGCC
GCAGCTCCGCGTGGACGGTGAGCTCGTCCCGCTCAAGACGGCGCCGCTCACGCCCGTGGAGACCAAGCAGCTCTGCTACT
CCATCCTCACGGACGCCCAGAAGCACAAGTTCGAGGAGGAGAACGAGCTGGACCTGTCGTTCGGCGTGAAGGGCCTGTCG
CGCTTTCGCGCGAACATCTTCATGCAGCGCGGCGCCGTCGCCGGCGCGTTCCGCACGATTCCGTTCAAGATCTTGACGTT
CCAGGAGCTGGGCCTGCCGCCGGTGGTGGCGGAGCTGGTGAAGAAGCCGCGCGGCCTCATCCTGGTGACGGGCCCCACGG
GCTCCGGCAAGTCCACCACGCTGGCCTCGATGATCGACAAGATCAACACCGAGCGTCATGAGCACATCATGACCATCGAG
GACCCCATCGAGTACCTGCACCCGCACAAGAACTGCCTGGTGAACCAGCGCGAGGTCGGTGCGGACACGCGCAACTTCAA
GACGGCCCTCAAGTACATCCTCCGCCAGGATCCGGACGTGGTGCTCGTCGGTGAGCTCCGAGACCTGGAGACCATCGAGG
CGGCGCTCACCATCGCGGAGACGGGCCACATCTGCTACGCGACGCTGCACACCAACAGCGCGGTGCAGACCATCAACCGC
ATCCTGGACGTCTTTCCCCCGTACCAGCAGCCCCAGGTGCGCGCCCAGCTGTCCTTCGTGCTCGAGGGCGTGATGAGCCA
GGCGCTCGTCGCCAAGGCGGGCGGCCCCGGCCGCGTGCTGGCCCTGGAGGTCATGGTGCCCAACCCCGCCATCCGGAACC
TCATCCGCGAGGACAAGGTCCACCAGATCTACTCCTCCATGCAGGTGGGCCAGGCGAAGTACGGCATGCAGACGTTCAAC
CAGGCGCTGGCCGCCCTTCTGGCGCGCCGGCTCATCTCCCAGGACGAGGCCTTCGGCCGTTCCTCCGACCCGGAGGAGCT
GCGCAACATCCTCGCCACGGGCGGCGCGCCGGGCGGAGCCCAGCGGCCGGCCGGGGGAGCGGGTGCCCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.824

98.378

0.5
  pilT Legionella pneumophila strain Lp02

54.328

90.541

0.492
  pilT Legionella pneumophila strain ERS1305867

54.328

90.541

0.492
  pilT Pseudomonas stutzeri DSM 10701

51.594

93.243

0.481
  pilT Pseudomonas aeruginosa PAK

51.594

93.243

0.481
  pilT Acinetobacter baylyi ADP1

51.304

93.243

0.478
  pilT Acinetobacter baumannii strain A118

51.014

93.243

0.476
  pilT Acinetobacter baumannii D1279779

51.014

93.243

0.476
  pilT Acinetobacter nosocomialis M2

50.725

93.243

0.473
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.102

90.27

0.443
  pilT Vibrio cholerae strain A1552

49.102

90.27

0.443
  pilT Neisseria gonorrhoeae MS11

45.161

92.162

0.416
  pilT Neisseria meningitidis 8013

45.161

92.162

0.416
  pilU Vibrio cholerae strain A1552

43.429

94.595

0.411
  pilU Pseudomonas stutzeri DSM 10701

41.292

96.216

0.397
  pilU Acinetobacter baylyi ADP1

40.634

93.784

0.381