Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NP781_RS03160 Genome accession   NZ_CP102383
Coordinates   682503..683549 (+) Length   348 a.a.
NCBI ID   WP_257521355.1    Uniprot ID   -
Organism   Lysinibacillus sp. FN11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 677503..688549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NP781_RS03145 (NP781_03145) - 678557..678919 (-) 363 WP_257521353.1 type II secretion system protein -
  NP781_RS03150 (NP781_03150) - 679472..680803 (+) 1332 WP_257521354.1 G5 domain-containing protein -
  NP781_RS03155 (NP781_03155) - 680821..682491 (+) 1671 WP_373880536.1 GspE/PulE family protein -
  NP781_RS03160 (NP781_03160) pilT 682503..683549 (+) 1047 WP_257521355.1 type IV pilus twitching motility protein PilT Machinery gene
  NP781_RS03165 (NP781_03165) - 683546..684751 (+) 1206 WP_257521356.1 type II secretion system F family protein -
  NP781_RS03170 (NP781_03170) - 684877..685353 (+) 477 WP_054550585.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NP781_RS03175 (NP781_03175) - 685410..685859 (+) 450 WP_257521357.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NP781_RS03180 (NP781_03180) pilM 685972..686946 (+) 975 WP_016991996.1 type IV pilus biogenesis protein PilM -
  NP781_RS03185 (NP781_03185) - 686946..687539 (+) 594 WP_257521358.1 PilN domain-containing protein -
  NP781_RS03190 (NP781_03190) - 687536..688228 (+) 693 WP_257521359.1 hypothetical protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38958.80 Da        Isoelectric Point: 6.9122

>NTDB_id=716740 NP781_RS03160 WP_257521355.1 682503..683549(+) (pilT) [Lysinibacillus sp. FN11]
MTAYSIVELLTRAFEEKASDLHLTKDIPPVYRIHGQLEHYGTVPLETEDLKEMVKVILPVHKQQEFEDKGETDFNFSLPG
KCRFRVNAYHQRNEVTIAARLIEAKIPSIEALNMPQVLYKLAEKPQGLILVTGPTGSGKSTTLAAMIDHINETRSKHIIT
LEDPIEYLHSHKKSVINQREIGVDTMSFANGLRASLRQDPDIILVGEMRDYETISTAITAAETGHLVMATLHTSSAPTTI
DRIIDVFPPHQQSQIRVQLANVLQGIVSQRLFVRKDVKGRIAATEILVQAPAISNLIRNEKIHQIPSVMQTSKALGMHTL
EYSMQHLITTGKISLEDARPYMNAGDYS

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=716740 NP781_RS03160 WP_257521355.1 682503..683549(+) (pilT) [Lysinibacillus sp. FN11]
ATGACAGCATATTCAATCGTTGAATTATTAACACGTGCATTTGAAGAGAAAGCATCGGATCTACATCTAACAAAAGATAT
TCCACCAGTGTATCGTATTCATGGGCAGCTGGAGCATTATGGCACAGTCCCACTTGAAACAGAGGATTTGAAGGAAATGG
TAAAGGTCATTCTCCCTGTACATAAACAACAGGAATTTGAGGACAAAGGAGAAACGGACTTTAACTTTTCATTGCCTGGG
AAATGTCGATTCCGTGTCAATGCTTACCATCAGCGAAATGAAGTAACGATTGCGGCTCGTTTAATTGAGGCTAAAATTCC
TTCTATTGAAGCGCTCAATATGCCACAAGTGTTATATAAGCTTGCGGAAAAACCACAAGGCTTAATTTTAGTGACTGGCC
CAACAGGCTCAGGAAAATCAACTACCTTGGCAGCAATGATTGATCATATTAATGAAACGAGATCAAAACATATTATTACA
TTAGAGGATCCAATCGAATACTTGCATTCACATAAGAAATCTGTCATCAATCAGCGTGAGATTGGTGTCGATACGATGAG
CTTTGCAAATGGTTTACGTGCATCACTACGTCAGGACCCAGATATTATTTTAGTCGGGGAGATGCGAGATTACGAAACCA
TTTCAACGGCTATTACAGCTGCTGAAACAGGACATTTAGTCATGGCTACACTTCATACAAGTAGTGCACCAACAACGATC
GACCGTATTATAGACGTATTTCCACCACATCAGCAAAGTCAAATTCGTGTGCAGCTTGCGAATGTACTGCAAGGTATTGT
TTCTCAGCGTTTATTTGTACGTAAGGATGTAAAAGGGCGTATTGCGGCGACAGAAATTTTAGTACAGGCACCTGCCATCT
CGAATTTAATTCGTAATGAAAAAATCCATCAAATTCCAAGTGTGATGCAGACAAGTAAAGCATTAGGTATGCACACATTG
GAATATTCGATGCAACACTTAATTACGACAGGTAAGATTTCACTTGAGGATGCACGACCATATATGAACGCTGGTGATTA
TAGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.786

96.552

0.5
  pilT Neisseria meningitidis 8013

49.701

95.977

0.477
  pilT Neisseria gonorrhoeae MS11

49.401

95.977

0.474
  pilT Acinetobacter baumannii D1279779

49.102

95.977

0.471
  pilT Acinetobacter nosocomialis M2

49.102

95.977

0.471
  pilT Acinetobacter baumannii strain A118

49.102

95.977

0.471
  pilT Pseudomonas aeruginosa PAK

48.802

95.977

0.468
  pilT Vibrio cholerae strain A1552

48.503

95.977

0.466
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.503

95.977

0.466
  pilT Legionella pneumophila strain ERS1305867

48.936

94.54

0.463
  pilT Legionella pneumophila strain Lp02

48.936

94.54

0.463
  pilT Pseudomonas stutzeri DSM 10701

47.904

95.977

0.46
  pilT Acinetobacter baylyi ADP1

47.605

95.977

0.457
  pilU Pseudomonas stutzeri DSM 10701

42.296

95.115

0.402
  pilU Vibrio cholerae strain A1552

41.925

92.529

0.388
  pilU Acinetobacter baylyi ADP1

39.759

95.402

0.379