Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NRF19_RS14370 Genome accession   NZ_CP102377
Coordinates   2569683..2570666 (+) Length   327 a.a.
NCBI ID   WP_327059594.1    Uniprot ID   -
Organism   Leclercia adecarboxylata strain M17     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2564683..2575666
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRF19_RS14340 (NRF19_14305) - 2565084..2566073 (-) 990 WP_220434763.1 TRAP transporter substrate-binding protein -
  NRF19_RS14345 (NRF19_14310) hemW 2566357..2567493 (-) 1137 WP_327059591.1 radical SAM family heme chaperone HemW -
  NRF19_RS14350 (NRF19_14315) - 2567486..2568079 (-) 594 WP_156239746.1 XTP/dITP diphosphatase -
  NRF19_RS14355 (NRF19_14320) yggU 2568083..2568379 (-) 297 WP_103822821.1 DUF167 family protein YggU -
  NRF19_RS14360 (NRF19_14325) - 2568376..2568942 (-) 567 WP_327059592.1 YggT family protein -
  NRF19_RS14365 (NRF19_14330) - 2568964..2569665 (-) 702 WP_327059593.1 YggS family pyridoxal phosphate-dependent enzyme -
  NRF19_RS14370 (NRF19_14335) pilT 2569683..2570666 (+) 984 WP_327059594.1 type IV pilus twitching motility protein PilT Machinery gene
  NRF19_RS14375 (NRF19_14340) - 2570759..2571454 (+) 696 WP_327059595.1 IclR family transcriptional regulator domain-containing protein -
  NRF19_RS14380 (NRF19_14345) ruvX 2571487..2571903 (-) 417 WP_103822816.1 Holliday junction resolvase RuvX -
  NRF19_RS14385 (NRF19_14350) - 2571903..2572463 (-) 561 WP_103176923.1 YqgE/AlgH family protein -
  NRF19_RS14390 (NRF19_14355) gshB 2572619..2573566 (-) 948 WP_327059596.1 glutathione synthase -
  NRF19_RS14395 (NRF19_14360) rsmE 2573586..2574317 (-) 732 WP_114316824.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NRF19_RS14400 (NRF19_14365) endA 2574402..2575109 (-) 708 WP_103176920.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 327 a.a.        Molecular weight: 35764.08 Da        Isoelectric Point: 7.2497

>NTDB_id=716636 NRF19_RS14370 WP_327059594.1 2569683..2570666(+) (pilT) [Leclercia adecarboxylata strain M17]
MDMEEIVALSVKHNVSDLHLCSNAPPRWRRRGKMELAPFPGPEVAMLLKSWLSDEQQGAWCAQGQVDFAVTLASKQRLRA
SAFAHMQGCSLALRLLPDKCPQLHTLGAPRAIPELLANDNGLILVTGATGSGKSTTLAAMVDFLNHHSDGHILTLEDPVE
FIHQSKRCLIQQREIGLHCLSFADALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDA
FPAQEKEPVRKQLAGSLRAVLAQKLERDNQQGRVALFELLVNTPAAANLIREGKTYQLPGVIQTGQQAGMQNFEQSLAER
RAQGRLS

Nucleotide


Download         Length: 984 bp        

>NTDB_id=716636 NRF19_RS14370 WP_327059594.1 2569683..2570666(+) (pilT) [Leclercia adecarboxylata strain M17]
ATGGATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAGTAACGCGCCACCCCG
CTGGCGGCGGCGAGGGAAAATGGAATTGGCCCCCTTTCCGGGGCCGGAGGTCGCGATGCTGCTGAAAAGCTGGCTCAGCG
ACGAGCAGCAGGGGGCATGGTGCGCCCAGGGTCAGGTGGATTTTGCCGTTACGCTTGCGAGCAAGCAGCGACTGCGGGCC
AGCGCTTTTGCACATATGCAGGGGTGTTCCCTGGCGCTCCGCCTGCTTCCAGACAAGTGCCCGCAGTTGCATACATTGGG
AGCGCCGCGGGCGATACCTGAACTGCTGGCAAATGATAACGGCCTGATACTGGTGACCGGGGCGACAGGCAGCGGTAAAT
CCACCACGCTTGCGGCGATGGTCGATTTTCTCAATCACCATAGCGACGGGCATATCCTGACGCTCGAAGATCCGGTGGAA
TTTATCCATCAGAGCAAGCGTTGCCTGATTCAGCAGCGGGAGATTGGCCTGCATTGCCTCTCCTTTGCCGACGCGCTGCG
GGCAGCATTACGTGAAGATCCCGATGTGATCCTGCTGGGCGAACTGCGTGACAGCGAGACGATCCGCCTTGCGCTTACCG
CCGCCGAGACCGGGCATCTGGTGCTGGCGACGCTTCACACCCGGGGTGCCGCCCAGGCAGTGGAGAGGCTGGTGGATGCC
TTTCCCGCGCAGGAAAAAGAGCCGGTACGTAAACAGCTGGCAGGCAGCCTGCGGGCCGTGCTGGCGCAAAAACTCGAAAG
GGATAACCAGCAGGGACGCGTCGCGCTGTTCGAGCTGCTGGTTAATACCCCAGCGGCGGCCAATTTGATCCGTGAAGGCA
AAACCTATCAACTGCCCGGCGTGATCCAGACCGGGCAGCAGGCTGGCATGCAAAACTTCGAGCAGAGCCTGGCTGAGCGC
AGGGCGCAGGGGCGATTGTCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.495
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.495
  pilT Neisseria meningitidis 8013

49.085

100

0.492
  pilT Neisseria gonorrhoeae MS11

48.78

100

0.489
  pilT Legionella pneumophila strain Lp02

48.137

98.471

0.474
  pilT Legionella pneumophila strain ERS1305867

48.137

98.471

0.474
  pilT Pseudomonas stutzeri DSM 10701

46.667

100

0.471
  pilT Acinetobacter baumannii D1279779

46.667

100

0.471
  pilT Acinetobacter baumannii strain A118

46.667

100

0.471
  pilT Acinetobacter nosocomialis M2

46.364

100

0.468
  pilT Pseudomonas aeruginosa PAK

46.364

100

0.468
  pilT Acinetobacter baylyi ADP1

46.483

100

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.478

98.471

0.428
  pilU Vibrio cholerae strain A1552

39.091

100

0.394
  pilU Pseudomonas stutzeri DSM 10701

37.764

100

0.382
  pilU Acinetobacter baylyi ADP1

37.92

100

0.379