Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NRG23_RS21310 Genome accession   NZ_CP102374
Coordinates   4728950..4729984 (+) Length   344 a.a.
NCBI ID   WP_053270126.1    Uniprot ID   A0AAQ0APH0
Organism   Pseudomonas sp. T8     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4723950..4734984
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRG23_RS21275 (NRG23_21275) - 4724030..4724464 (-) 435 WP_028683555.1 DUF4426 domain-containing protein -
  NRG23_RS21280 (NRG23_21280) metW 4724492..4725112 (-) 621 WP_007921185.1 methionine biosynthesis protein MetW -
  NRG23_RS21285 (NRG23_21285) - 4725120..4726259 (-) 1140 WP_009051317.1 homoserine O-acetyltransferase -
  NRG23_RS21290 (NRG23_21290) - 4726433..4726726 (-) 294 WP_028683556.1 DUF167 domain-containing protein -
  NRG23_RS21295 (NRG23_21295) - 4726740..4727330 (-) 591 WP_009051315.1 YggT family protein -
  NRG23_RS21300 (NRG23_21300) proC 4727341..4728159 (-) 819 WP_257372021.1 pyrroline-5-carboxylate reductase -
  NRG23_RS21305 (NRG23_21305) - 4728194..4728892 (-) 699 WP_009051313.1 YggS family pyridoxal phosphate-dependent enzyme -
  NRG23_RS21310 (NRG23_21310) pilT 4728950..4729984 (+) 1035 WP_053270126.1 type IV pilus twitching motility protein PilT Machinery gene
  NRG23_RS21315 (NRG23_21315) - 4730067..4730699 (-) 633 WP_009046022.1 C40 family peptidase -
  NRG23_RS21320 (NRG23_21320) - 4730950..4731369 (+) 420 WP_009051311.1 TM2 domain-containing protein -
  NRG23_RS21325 (NRG23_21325) - 4731475..4732746 (-) 1272 WP_028683559.1 dihydroorotase -
  NRG23_RS21330 (NRG23_21330) - 4732743..4733747 (-) 1005 WP_007929978.1 aspartate carbamoyltransferase catalytic subunit -
  NRG23_RS21335 (NRG23_21335) pyrR 4733774..4734280 (-) 507 WP_053270124.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  NRG23_RS21340 (NRG23_21340) ruvX 4734402..4734839 (-) 438 WP_009046019.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37522.21 Da        Isoelectric Point: 6.9827

>NTDB_id=716611 NRG23_RS21310 WP_053270126.1 4728950..4729984(+) (pilT) [Pseudomonas sp. T8]
MDITELLAFSVKQGASDLHLSAGLPPMIRIDGDIRRINLPVLEAQQVHELILASMTDPQRAEFKAALEIDFSFAVPGLAR
FRVNAFNHQRGAGAVFRSIPAKVPSLDELGMGEVFRRISEVPRGLVLVTGPTGSGKSTTLAAMIDHLNQSRHQHILTIED
PIEFIHESKKCLINQREVHRDTHSFSTALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTVDRV
VDVFPAEEKAMVRSMLSESLQAVISQALVKRIGGGRVAAHEIMLGSAAIRNLIREDKVAQMYSAIQTGGAQGMQTLDMSL
KALVDAGLVSREHAREKARAPESI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=716611 NRG23_RS21310 WP_053270126.1 4728950..4729984(+) (pilT) [Pseudomonas sp. T8]
ATGGATATCACTGAGTTGCTGGCCTTCAGCGTCAAACAGGGCGCTTCGGACCTGCACCTCTCGGCCGGTTTGCCGCCGAT
GATTCGCATCGACGGCGATATTCGCCGGATCAACCTGCCGGTGCTGGAGGCGCAACAGGTGCACGAGCTGATCCTGGCCA
GCATGACGGACCCGCAGCGGGCCGAGTTCAAGGCCGCGCTGGAGATCGACTTTTCCTTCGCGGTGCCGGGCCTGGCGCGC
TTTCGGGTCAATGCCTTCAACCATCAGCGCGGCGCTGGCGCGGTCTTTCGCAGCATTCCGGCCAAGGTGCCGAGCCTGGA
CGAACTGGGCATGGGCGAGGTGTTTCGCCGCATCAGCGAAGTGCCCCGCGGCCTGGTACTGGTGACCGGACCGACCGGTT
CGGGCAAGTCGACCACCCTGGCGGCGATGATCGATCACCTGAACCAGAGCCGGCACCAGCACATCCTCACCATCGAGGAC
CCCATCGAGTTCATCCACGAATCGAAGAAGTGCCTGATCAACCAGCGCGAGGTGCATCGCGACACCCACAGCTTCTCCAC
GGCGCTGCGTTCGGCCCTGCGCGAGGACCCGGATGTGATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATTCGCCTGG
CGTTGACCGCCGCGGAGACCGGGCATCTGGTGTTCGGTACCCTGCACACCACCTCGGCGGCGAAAACCGTGGACCGGGTG
GTGGATGTGTTTCCGGCCGAGGAGAAGGCGATGGTCCGCTCCATGCTCTCTGAATCGCTGCAGGCGGTGATTTCCCAGGC
GCTGGTGAAGCGGATCGGCGGAGGCCGGGTAGCGGCCCACGAGATCATGCTGGGCAGCGCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGCGGGGCGCAGGGGATGCAGACCCTGGACATGAGCCTG
AAGGCGCTGGTGGATGCCGGGCTGGTCAGCCGGGAGCACGCCCGGGAGAAGGCGCGGGCGCCGGAGAGTATTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

81.395

100

0.814
  pilT Pseudomonas stutzeri DSM 10701

78.488

100

0.785
  pilT Acinetobacter nosocomialis M2

74.419

100

0.744
  pilT Acinetobacter baumannii D1279779

74.419

100

0.744
  pilT Acinetobacter baumannii strain A118

74.419

100

0.744
  pilT Acinetobacter baylyi ADP1

72.965

100

0.73
  pilT Legionella pneumophila strain Lp02

68.047

98.256

0.669
  pilT Legionella pneumophila strain ERS1305867

68.047

98.256

0.669
  pilT Neisseria meningitidis 8013

65.797

100

0.66
  pilT Neisseria gonorrhoeae MS11

65.507

100

0.657
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.469

97.965

0.651
  pilT Vibrio cholerae strain A1552

66.469

97.965

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.312

99.709

0.512
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404
  pilU Vibrio cholerae strain A1552

41.194

97.384

0.401
  pilU Pseudomonas stutzeri DSM 10701

39.701

97.384

0.387