Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NRZ32_RS15280 Genome accession   NZ_CP102368
Coordinates   3388422..3389456 (+) Length   344 a.a.
NCBI ID   WP_005305010.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain BC18     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3383422..3394456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRZ32_RS15250 (NRZ32_15250) - 3385093..3385404 (+) 312 WP_005304992.1 hypothetical protein -
  NRZ32_RS15255 (NRZ32_15255) - 3385467..3385886 (-) 420 WP_005304995.1 DUF4426 domain-containing protein -
  NRZ32_RS15260 (NRZ32_15260) yggU 3385905..3386204 (-) 300 WP_005304998.1 DUF167 family protein YggU -
  NRZ32_RS15265 (NRZ32_15265) - 3386204..3386755 (-) 552 WP_017780458.1 YggT family protein -
  NRZ32_RS15270 (NRZ32_15270) proC 3386796..3387620 (-) 825 WP_017780457.1 pyrroline-5-carboxylate reductase -
  NRZ32_RS15275 (NRZ32_15275) - 3387681..3388382 (-) 702 WP_017780456.1 YggS family pyridoxal phosphate-dependent enzyme -
  NRZ32_RS15280 (NRZ32_15280) pilT 3388422..3389456 (+) 1035 WP_005305010.1 type IVa pilus ATPase TapT Machinery gene
  NRZ32_RS15285 (NRZ32_15285) pilU 3389485..3390594 (+) 1110 WP_010636179.1 type IVa pilus ATPase TapU Machinery gene
  NRZ32_RS15290 (NRZ32_15290) yaaA 3390626..3391399 (+) 774 WP_042007907.1 peroxide stress protein YaaA -
  NRZ32_RS15295 (NRZ32_15295) srmB 3391576..3392799 (-) 1224 WP_042007906.1 ATP-dependent RNA helicase SrmB -
  NRZ32_RS15300 (NRZ32_15300) - 3392928..3393638 (+) 711 WP_268037524.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38319.12 Da        Isoelectric Point: 6.7439

>NTDB_id=716557 NRZ32_RS15280 WP_005305010.1 3388422..3389456(+) (pilT) [Aeromonas dhakensis strain BC18]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEYPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGMVAALDAKAKAVDPNTI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=716557 NRZ32_RS15280 WP_005305010.1 3388422..3389456(+) (pilT) [Aeromonas dhakensis strain BC18]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAACATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGCGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAGCACCGGGAAGTACACGCCCTCATCTATGACA
TCATGAACGACCACCAGCGCAAGGAACTGGAAGAGAACTTCGAGGTGGACTTCTCATTCGAGGTGCCGAACCTGGCCCGC
TTCCGGGTCAACGCCTTCCAGCAGGCGCGCGGCTCCGGCGCGGTATTTCGGACCATCCCCAGCACAGTGCTGAGCCTGGA
AGATCTTGATGCCCCGGAGATCTTTCGCAAGATTGCCGAATACCCGCGCGGCCTGGTGCTGGTGACCGGTCCGACCGGTT
CGGGCAAGTCCACTACCTTGGCGGCCATGGTCAACTACATCAACGAGAACTTCCATCACCACATCCTCACCATCGAGGAT
CCCATCGAATTCGTGCACGAAAACAAACGCTGCCTGGTAAACCAGCGGGAAGTGCACCGCGACACCAAGAGCTTCAGCAA
CGCCTTGCGTTCGGCGCTGCGGGAAGACCCGGACATCATACTGGTGGGCGAGATGCGCGATCTGGAAACCATTCGTCTGG
CCATGACCGCGGCCGAGACCGGTCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGGATC
ATCGACGTCTTCCCCGGTGCGGAAAAAGACATGGTGCGCTCCATGCTCTCCGAATCGTTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGCATCGGCGGCGGCCGGGTAGCGGCCCACGAGATCATGATGGGTATTCCTGCCGTGCGAAACCTCATAC
GGGAGGACAAGATCGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGCCGCGGCATGGTGGCGGCGCTGGATGCCAAGGCCAAGGCGGTCGATCCCAACACCATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Acinetobacter nosocomialis M2

77.035

100

0.77
  pilT Acinetobacter baumannii D1279779

76.744

100

0.767
  pilT Acinetobacter baumannii strain A118

76.744

100

0.767
  pilT Pseudomonas stutzeri DSM 10701

76.833

99.128

0.762
  pilT Pseudomonas aeruginosa PAK

75.953

99.128

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.261

97.965

0.747
  pilT Vibrio cholerae strain A1552

76.261

97.965

0.747
  pilT Legionella pneumophila strain Lp02

72.647

98.837

0.718
  pilT Legionella pneumophila strain ERS1305867

72.647

98.837

0.718
  pilT Neisseria meningitidis 8013

70.796

98.547

0.698
  pilT Neisseria gonorrhoeae MS11

70.501

98.547

0.695
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407