Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NQ186_RS27960 Genome accession   NZ_CP102358
Coordinates   6270367..6271401 (-) Length   344 a.a.
NCBI ID   WP_041063525.1    Uniprot ID   A0A2A2PM05
Organism   Pseudomonas zeae strain BIM B-582     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6265367..6276401
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQ186_RS27930 (NQ186_27930) ruvX 6265500..6265937 (+) 438 WP_064589634.1 Holliday junction resolvase RuvX -
  NQ186_RS27935 (NQ186_27935) pyrR 6266018..6266524 (+) 507 WP_034153653.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  NQ186_RS27940 (NQ186_27940) - 6266552..6267556 (+) 1005 WP_007913665.1 aspartate carbamoyltransferase catalytic subunit -
  NQ186_RS27945 (NQ186_27945) - 6267553..6268824 (+) 1272 WP_093429839.1 dihydroorotase -
  NQ186_RS27950 (NQ186_27950) - 6268918..6269349 (-) 432 WP_007913651.1 TM2 domain-containing protein -
  NQ186_RS27955 (NQ186_27955) - 6269598..6270215 (+) 618 WP_016985978.1 C40 family peptidase -
  NQ186_RS27960 (NQ186_27960) pilT 6270367..6271401 (-) 1035 WP_041063525.1 type IV pilus twitching motility protein PilT Machinery gene
  NQ186_RS27965 (NQ186_27965) - 6271458..6272144 (+) 687 WP_257356536.1 YggS family pyridoxal phosphate-dependent enzyme -
  NQ186_RS27970 (NQ186_27970) proC 6272168..6272986 (+) 819 WP_093429835.1 pyrroline-5-carboxylate reductase -
  NQ186_RS27975 (NQ186_27975) - 6272997..6273587 (+) 591 WP_041063533.1 YggT family protein -
  NQ186_RS27980 (NQ186_27980) - 6273584..6273880 (+) 297 WP_007913642.1 DUF167 domain-containing protein -
  NQ186_RS27985 (NQ186_27985) - 6274108..6275247 (+) 1140 WP_257356537.1 homoserine O-acetyltransferase -
  NQ186_RS27990 (NQ186_27990) metW 6275255..6275875 (+) 621 WP_257356538.1 methionine biosynthesis protein MetW -
  NQ186_RS27995 (NQ186_27995) - 6275902..6276336 (+) 435 WP_257356539.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38085.76 Da        Isoelectric Point: 7.0158

>NTDB_id=716415 NQ186_RS27960 WP_041063525.1 6270367..6271401(-) (pilT) [Pseudomonas zeae strain BIM B-582]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLAMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KELVTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=716415 NQ186_RS27960 WP_041063525.1 6270367..6271401(-) (pilT) [Pseudomonas zeae strain BIM B-582]
ATGGATATCACTGAACTGCTGGCCTTCAGCGCCAAACAGGGAGCTTCCGACCTGCACCTGTCGGCCGGTCTGCCGCCGAT
GATCCGCGTCGATGGCGACGTGCGGCGGATCAATCTGCCGGCGCTGGATCACAAGCAAGTGCACGAACTGATCTACGACA
TCATGAACGACACCCAGCGGGTCGACTTCGAGAAACATCTGGAAACCGACTTTTCTTTCGAAGTGCCGGGTGTCGCGCGT
TTCCGGGTTAACGCCTTTAACCAGAACCGTGGCGCCGGCGCCGTATTCCGTACGATTCCGTCGAAAGTGCTGAGCATGGA
AGACCTTGCCATGGGCGACGTCTTCCGCAAGATCACCGATGCGCCGCGCGGTCTGGTGTTGGTCACCGGGCCGACCGGTT
CCGGCAAATCGACCACGCTGGCGGCGATGATCGATTACCTCAATACCCACCGGCATCACCACATCCTGACTATCGAAGAT
CCGATCGAGTTTGTTCACGAATCGCGCAAATGCCTGATCAATCAGCGTGAAGTCCACCGTGATACCCGCAGTTTCGCCAC
TGCGTTGCGCTCGGCACTGCGTGAAGACCCGGATGTAATTTTGGTCGGTGAGATGCGTGACCTGGAAACCATTCGCCTGG
CGCTGACCGCAGCCGAAACCGGGCACTTGGTGTTCGGCACGCTGCACACCACCTCGGCGGCGAAAACCATCGACCGCGTA
GTCGACGTGTTCCCCGGTGACGAGAAGTCGATGGTGCGCTCGATGCTTTCCGAGTCGCTGCTGGCGGTGGTCTCGCAGAC
GCTGATCAAGAAGATCGGTGGCGGGCGGGTGGCGGCGCACGAGATCATGTTGGGGACGTCGGCGATCCGTAACCTGATCC
GTGAGGACAAGGTCGCGCAGATGTATTCGGCGATTCAGACGGGTGGGTCGTTGGGCATGCAGACACTGGACATGTGCCTG
AAGGAACTGGTGACCAAGGGCTTGATCAGCCGCGAACATGCGCGGGAGAAGGCGCGCACGCCGGATAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A2PM05

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

87.791

100

0.878
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

79.651

100

0.797
  pilT Acinetobacter baumannii D1279779

79.651

100

0.797
  pilT Acinetobacter baumannii strain A118

79.651

100

0.797
  pilT Acinetobacter baylyi ADP1

77.616

100

0.776
  pilT Legionella pneumophila strain Lp02

72.781

98.256

0.715
  pilT Legionella pneumophila strain ERS1305867

72.781

98.256

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404
  pilU Vibrio cholerae strain A1552

39.71

100

0.398