Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NQ196_RS09850 Genome accession   NZ_CP102311
Coordinates   2250103..2251155 (-) Length   350 a.a.
NCBI ID   WP_061428819.1    Uniprot ID   A0A127EK00
Organism   Clostridium perfringens strain JXNC-S10     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2244848..2284277 2250103..2251155 within 0


Gene organization within MGE regions


Location: 2244848..2284277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQ196_RS09825 (NQ196_09825) sigG 2244848..2245621 (-) 774 WP_003449454.1 RNA polymerase sporulation sigma factor SigG -
  NQ196_RS09830 (NQ196_09830) sigE 2245696..2246403 (-) 708 WP_003449468.1 RNA polymerase sporulation sigma factor SigE -
  NQ196_RS09835 (NQ196_09835) spoIIGA 2246417..2247223 (-) 807 WP_257261457.1 sigma-E processing peptidase SpoIIGA -
  NQ196_RS09840 (NQ196_09840) ftsZ 2247628..2248773 (-) 1146 WP_111916955.1 cell division protein FtsZ -
  NQ196_RS09845 (NQ196_09845) - 2248787..2250025 (-) 1239 WP_257261458.1 cell division FtsA domain-containing protein -
  NQ196_RS09850 (NQ196_09850) pilT 2250103..2251155 (-) 1053 WP_061428819.1 type IV pilus twitching motility protein PilT Machinery gene
  NQ196_RS09855 (NQ196_09855) - 2251336..2251608 (-) 273 Protein_1918 sigma-70 family RNA polymerase sigma factor -
  NQ196_RS09860 (NQ196_09860) - 2251715..2252122 (-) 408 WP_257261459.1 hypothetical protein -
  NQ196_RS09865 (NQ196_09865) - 2252253..2252636 (+) 384 WP_224377326.1 helix-turn-helix domain-containing protein -
  NQ196_RS09870 (NQ196_09870) - 2252667..2253677 (-) 1011 WP_257261460.1 GH25 family lysozyme -
  NQ196_RS09875 (NQ196_09875) - 2253797..2254048 (-) 252 WP_057231815.1 phage holin family protein -
  NQ196_RS09880 (NQ196_09880) - 2254048..2254365 (-) 318 WP_115635151.1 hypothetical protein -
  NQ196_RS09885 (NQ196_09885) - 2254458..2257184 (-) 2727 WP_257261461.1 M14 family zinc carboxypeptidase -
  NQ196_RS09890 (NQ196_09890) - 2257198..2257473 (-) 276 WP_257261462.1 hypothetical protein -
  NQ196_RS09895 (NQ196_09895) - 2257486..2259045 (-) 1560 WP_257261463.1 phage baseplate upper protein -
  NQ196_RS09900 (NQ196_09900) - 2259095..2262094 (-) 3000 WP_257261464.1 phage tail spike protein -
  NQ196_RS09905 (NQ196_09905) - 2262095..2262802 (-) 708 WP_118429558.1 distal tail protein Dit -
  NQ196_RS09910 (NQ196_09910) - 2262796..2265531 (-) 2736 WP_257261465.1 phage tail tape measure protein -
  NQ196_RS09915 (NQ196_09915) - 2265631..2265927 (-) 297 WP_257261466.1 hypothetical protein -
  NQ196_RS09920 (NQ196_09920) gpG 2266137..2266451 (-) 315 WP_257261467.1 phage tail assembly chaperone G -
  NQ196_RS09925 (NQ196_09925) - 2266470..2267078 (-) 609 WP_257261468.1 major tail protein -
  NQ196_RS09930 (NQ196_09930) - 2267098..2267448 (-) 351 WP_118429553.1 hypothetical protein -
  NQ196_RS09935 (NQ196_09935) - 2267450..2267899 (-) 450 WP_257261469.1 HK97-gp10 family putative phage morphogenesis protein -
  NQ196_RS09940 (NQ196_09940) - 2267900..2268235 (-) 336 WP_371745520.1 phage head closure protein -
  NQ196_RS09945 (NQ196_09945) - 2268228..2268512 (-) 285 WP_164807503.1 head-tail connector protein -
  NQ196_RS09950 (NQ196_09950) - 2268533..2269618 (-) 1086 WP_016048843.1 phage major capsid protein -
  NQ196_RS09955 (NQ196_09955) - 2269620..2270273 (-) 654 WP_016048875.1 HK97 family phage prohead protease -
  NQ196_RS09960 (NQ196_09960) - 2270266..2271474 (-) 1209 WP_164807502.1 phage portal protein -
  NQ196_RS09965 (NQ196_09965) - 2271669..2273348 (-) 1680 WP_016048840.1 terminase large subunit -
  NQ196_RS09970 (NQ196_09970) - 2273345..2273722 (-) 378 WP_016048839.1 phage terminase small subunit P27 family -
  NQ196_RS09975 (NQ196_09975) - 2273848..2274309 (-) 462 WP_016048874.1 hypothetical protein -
  NQ196_RS09980 (NQ196_09980) - 2274954..2275091 (+) 138 WP_164803877.1 DUF6364 family protein -
  NQ196_RS09985 (NQ196_09985) - 2275399..2275881 (-) 483 WP_111925975.1 hypothetical protein -
  NQ196_RS09990 (NQ196_09990) - 2275933..2276166 (-) 234 WP_224383526.1 hypothetical protein -
  NQ196_RS09995 (NQ196_09995) - 2276258..2276584 (-) 327 WP_257261471.1 hypothetical protein -
  NQ196_RS10000 (NQ196_10000) - 2276591..2276923 (-) 333 WP_221366700.1 hypothetical protein -
  NQ196_RS10005 (NQ196_10005) - 2276984..2277910 (-) 927 WP_257261472.1 hypothetical protein -
  NQ196_RS10010 (NQ196_10010) - 2278102..2278380 (-) 279 WP_257261473.1 helix-turn-helix domain-containing protein -
  NQ196_RS10015 (NQ196_10015) - 2278458..2278676 (-) 219 WP_257261474.1 hypothetical protein -
  NQ196_RS10020 (NQ196_10020) - 2278679..2279419 (-) 741 WP_016048863.1 hypothetical protein -
  NQ196_RS10025 (NQ196_10025) - 2279648..2280043 (-) 396 WP_016048862.1 HNH endonuclease -
  NQ196_RS10030 (NQ196_10030) - 2280030..2280203 (-) 174 WP_164807498.1 hypothetical protein -
  NQ196_RS10035 (NQ196_10035) - 2280240..2280428 (-) 189 WP_016048877.1 hypothetical protein -
  NQ196_RS10040 (NQ196_10040) - 2280534..2280665 (-) 132 WP_257261475.1 hypothetical protein -
  NQ196_RS10045 (NQ196_10045) - 2280778..2281005 (-) 228 WP_257261476.1 helix-turn-helix transcriptional regulator -
  NQ196_RS10050 (NQ196_10050) - 2281249..2281611 (+) 363 WP_016048876.1 helix-turn-helix domain-containing protein -
  NQ196_RS10055 (NQ196_10055) - 2281969..2282187 (+) 219 WP_224383538.1 helix-turn-helix domain-containing protein -
  NQ196_RS10060 (NQ196_10060) - 2282277..2283785 (+) 1509 WP_164807496.1 recombinase family protein -
  NQ196_RS10065 (NQ196_10065) - 2283831..2284277 (-) 447 Protein_1960 sigma-70 family RNA polymerase sigma factor -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39004.68 Da        Isoelectric Point: 5.4480

>NTDB_id=716036 NQ196_RS09850 WP_061428819.1 2250103..2251155(-) (pilT) [Clostridium perfringens strain JXNC-S10]
MQSLAELLELTVKEGASDLHLTVGISPIIKVNGKLVRLEHEILRPEDTEAYAREILQDAYEKYDAIGEYDTSYSIHGKGR
FRVNIYKQRNSTALAIRVISLDMPTLDSLGYPETLKDICNLKRGLVLVTGPTGSGKSTTLAALINEINSNRESHIITIED
PIEFLHKHNKSIVNQREIGKDTLSYERALKAALREDPDVILIGEMRDLETISTAITAAETGHLVFSTLHTIGAAKTIDRI
VDVFPPHQQEQIKIQLASVLQTIISQQLVETVDGDRTAALEIMVATPAIKNLIREGKTHQIESSIQTGSKYGMRTMDMEL
ANLYREGIITQETAMNSAIDREILSRLLMY

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=716036 NQ196_RS09850 WP_061428819.1 2250103..2251155(-) (pilT) [Clostridium perfringens strain JXNC-S10]
ATGCAAAGTTTAGCAGAACTTTTAGAGTTAACAGTTAAAGAAGGAGCATCTGATTTGCATTTAACAGTAGGTATTTCTCC
TATAATAAAAGTTAATGGAAAATTAGTAAGGTTAGAACATGAAATATTACGCCCTGAAGATACTGAAGCGTATGCTAGAG
AGATATTACAGGATGCATATGAAAAGTATGATGCTATTGGAGAATACGATACATCATATTCAATACATGGTAAAGGTAGA
TTTAGAGTTAATATATATAAACAAAGAAATAGTACAGCTTTAGCTATAAGAGTTATATCTTTAGATATGCCAACTTTGGA
TAGTTTAGGATATCCTGAGACTTTAAAGGATATATGTAATCTTAAAAGAGGACTTGTATTAGTTACAGGACCTACTGGAA
GTGGTAAAAGTACAACTTTAGCAGCTTTAATAAATGAAATAAATAGTAATAGAGAATCTCATATAATAACAATAGAAGAT
CCTATAGAGTTTCTTCATAAACATAATAAATCTATTGTAAACCAAAGAGAGATAGGCAAGGACACTCTAAGTTATGAAAG
AGCCTTAAAAGCAGCTCTTAGAGAGGATCCTGATGTTATTTTAATTGGAGAAATGAGAGATTTAGAGACTATTTCAACAG
CTATAACAGCGGCAGAGACTGGACACTTAGTATTTTCAACTCTTCATACTATAGGAGCAGCTAAGACTATAGATAGAATA
GTAGATGTTTTTCCACCACATCAACAAGAGCAAATAAAAATACAGTTAGCTTCCGTTTTACAAACAATTATATCACAGCA
GTTAGTAGAAACAGTTGATGGAGATAGAACTGCGGCATTAGAAATAATGGTTGCTACACCAGCTATAAAAAATTTAATAA
GAGAAGGAAAAACTCATCAAATAGAATCATCCATACAAACAGGAAGTAAATATGGAATGAGAACCATGGACATGGAACTT
GCTAATCTGTATAGAGAAGGTATAATAACACAAGAAACGGCAATGAATTCTGCTATAGATAGGGAGATTTTAAGTCGATT
ATTGATGTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A127EK00

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.711

98.857

0.491
  pilT Neisseria meningitidis 8013

49.709

98.286

0.489
  pilT Neisseria gonorrhoeae MS11

49.709

98.286

0.489
  pilT Pseudomonas aeruginosa PAK

50.298

96

0.483
  pilT Legionella pneumophila strain Lp02

49.415

97.714

0.483
  pilT Legionella pneumophila strain ERS1305867

49.415

97.714

0.483
  pilT Pseudomonas stutzeri DSM 10701

50

96

0.48
  pilT Acinetobacter baylyi ADP1

50.602

94.857

0.48
  pilT Acinetobacter baumannii D1279779

49.702

96

0.477
  pilT Acinetobacter nosocomialis M2

49.702

96

0.477
  pilT Acinetobacter baumannii strain A118

49.702

96

0.477
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

93.429

0.474
  pilT Vibrio cholerae strain A1552

50.765

93.429

0.474
  pilU Pseudomonas stutzeri DSM 10701

43.976

94.857

0.417
  pilU Vibrio cholerae strain A1552

40.653

96.286

0.391
  pilU Acinetobacter baylyi ADP1

39.589

97.429

0.386