Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NQ194_RS10155 Genome accession   NZ_CP102300
Coordinates   2260118..2261170 (-) Length   350 a.a.
NCBI ID   WP_003451114.1    Uniprot ID   A0A173YF66
Organism   Clostridium perfringens strain JXNC-3B     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2254864..2295021 2260118..2261170 within 0


Gene organization within MGE regions


Location: 2254864..2295021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQ194_RS10130 (NQ194_10130) sigG 2254864..2255637 (-) 774 WP_003449454.1 RNA polymerase sporulation sigma factor SigG -
  NQ194_RS10135 (NQ194_10135) sigE 2255712..2256419 (-) 708 WP_003449468.1 RNA polymerase sporulation sigma factor SigE -
  NQ194_RS10140 (NQ194_10140) spoIIGA 2256433..2257239 (-) 807 WP_003458523.1 sigma-E processing peptidase SpoIIGA -
  NQ194_RS10145 (NQ194_10145) ftsZ 2257643..2258788 (-) 1146 WP_003449411.1 cell division protein FtsZ -
  NQ194_RS10150 (NQ194_10150) - 2258802..2260040 (-) 1239 WP_003478307.1 cell division FtsA domain-containing protein -
  NQ194_RS10155 (NQ194_10155) pilT 2260118..2261170 (-) 1053 WP_003451114.1 type IV pilus twitching motility protein PilT Machinery gene
  NQ194_RS10160 (NQ194_10160) - 2261351..2261629 (-) 279 Protein_1979 sigma-70 family RNA polymerase sigma factor -
  NQ194_RS10165 (NQ194_10165) - 2261709..2262077 (-) 369 WP_221366683.1 hypothetical protein -
  NQ194_RS10170 (NQ194_10170) - 2262172..2263200 (-) 1029 WP_257269235.1 GH25 family lysozyme -
  NQ194_RS10175 (NQ194_10175) - 2263253..2263735 (-) 483 WP_257269236.1 cobalt ABC transporter permease -
  NQ194_RS10180 (NQ194_10180) - 2263756..2263989 (-) 234 WP_011590771.1 hemolysin XhlA family protein -
  NQ194_RS10185 (NQ194_10185) - 2264073..2266730 (-) 2658 WP_257269237.1 SGNH/GDSL hydrolase family protein -
  NQ194_RS10190 (NQ194_10190) - 2266753..2268630 (-) 1878 WP_257269239.1 BppU family phage baseplate upper protein -
  NQ194_RS10195 (NQ194_10195) - 2268678..2271656 (-) 2979 WP_257269241.1 phage tail spike protein -
  NQ194_RS10200 (NQ194_10200) - 2271660..2272355 (-) 696 WP_257269242.1 distal tail protein Dit -
  NQ194_RS10205 (NQ194_10205) - 2272336..2275224 (-) 2889 WP_257269243.1 phage tail tape measure protein -
  NQ194_RS10210 (NQ194_10210) - 2275242..2275580 (-) 339 WP_257269244.1 hypothetical protein -
  NQ194_RS10215 (NQ194_10215) gpG 2275742..2276047 (-) 306 WP_011010266.1 phage tail assembly chaperone G -
  NQ194_RS10220 (NQ194_10220) - 2276099..2276701 (-) 603 WP_257269245.1 major tail protein -
  NQ194_RS10225 (NQ194_10225) - 2276714..2277064 (-) 351 WP_257269246.1 hypothetical protein -
  NQ194_RS10230 (NQ194_10230) - 2277074..2277532 (-) 459 WP_257269247.1 HK97-gp10 family putative phage morphogenesis protein -
  NQ194_RS10235 (NQ194_10235) - 2277532..2277861 (-) 330 WP_257269248.1 phage head closure protein -
  NQ194_RS10240 (NQ194_10240) - 2277854..2278132 (-) 279 WP_257269249.1 head-tail connector protein -
  NQ194_RS10245 (NQ194_10245) - 2278144..2279328 (-) 1185 WP_257269251.1 phage major capsid protein -
  NQ194_RS10250 (NQ194_10250) - 2279370..2279975 (-) 606 WP_118429549.1 HK97 family phage prohead protease -
  NQ194_RS10255 (NQ194_10255) - 2279965..2281215 (-) 1251 WP_257269252.1 phage portal protein -
  NQ194_RS10260 (NQ194_10260) - 2281212..2282951 (-) 1740 WP_257269254.1 terminase large subunit -
  NQ194_RS10265 (NQ194_10265) - 2282944..2283447 (-) 504 WP_118429547.1 phage terminase small subunit P27 family -
  NQ194_RS10270 (NQ194_10270) - 2283555..2283968 (-) 414 WP_257269257.1 HNH endonuclease -
  NQ194_RS10275 (NQ194_10275) - 2283949..2284245 (-) 297 WP_163235936.1 hypothetical protein -
  NQ194_RS10280 (NQ194_10280) - 2284250..2284423 (-) 174 WP_257269258.1 hypothetical protein -
  NQ194_RS10285 (NQ194_10285) - 2284590..2285138 (-) 549 WP_057231849.1 site-specific integrase -
  NQ194_RS10290 (NQ194_10290) - 2285184..2285351 (-) 168 WP_257269259.1 hypothetical protein -
  NQ194_RS10300 (NQ194_10300) - 2285643..2285777 (-) 135 WP_257269260.1 hypothetical protein -
  NQ194_RS10305 (NQ194_10305) - 2285774..2285980 (-) 207 WP_118429543.1 hypothetical protein -
  NQ194_RS10310 (NQ194_10310) - 2286121..2286576 (-) 456 WP_257269262.1 RNA polymerase subunit sigma -
  NQ194_RS10315 (NQ194_10315) - 2286720..2287163 (-) 444 WP_257269264.1 hypothetical protein -
  NQ194_RS10320 (NQ194_10320) - 2287191..2288120 (-) 930 WP_257269266.1 DNA cytosine methyltransferase -
  NQ194_RS10325 (NQ194_10325) - 2288131..2288406 (-) 276 WP_257269267.1 helix-turn-helix domain-containing protein -
  NQ194_RS10330 (NQ194_10330) - 2288470..2288859 (-) 390 WP_257269269.1 single-stranded DNA-binding protein -
  NQ194_RS10335 (NQ194_10335) - 2288874..2289020 (-) 147 WP_164792735.1 hypothetical protein -
  NQ194_RS10340 (NQ194_10340) - 2289032..2290312 (-) 1281 WP_163235919.1 DnaB-like helicase C-terminal domain-containing protein -
  NQ194_RS10345 (NQ194_10345) - 2290325..2291065 (-) 741 WP_163235917.1 conserved phage C-terminal domain-containing protein -
  NQ194_RS10350 (NQ194_10350) - 2291092..2291250 (-) 159 WP_163235915.1 hypothetical protein -
  NQ194_RS10355 (NQ194_10355) - 2291253..2291432 (-) 180 WP_163235913.1 hypothetical protein -
  NQ194_RS10360 (NQ194_10360) - 2291541..2291672 (-) 132 WP_257269271.1 hypothetical protein -
  NQ194_RS10365 (NQ194_10365) - 2291732..2291875 (-) 144 WP_163235911.1 hypothetical protein -
  NQ194_RS10370 (NQ194_10370) - 2292011..2292385 (+) 375 WP_163235909.1 helix-turn-helix domain-containing protein -
  NQ194_RS10375 (NQ194_10375) - 2292724..2292933 (+) 210 WP_257269272.1 helix-turn-helix domain-containing protein -
  NQ194_RS10380 (NQ194_10380) - 2293021..2294529 (+) 1509 WP_163235905.1 recombinase family protein -
  NQ194_RS10385 (NQ194_10385) - 2294575..2295021 (-) 447 Protein_2023 sigma-70 family RNA polymerase sigma factor -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39029.73 Da        Isoelectric Point: 5.4480

>NTDB_id=716018 NQ194_RS10155 WP_003451114.1 2260118..2261170(-) (pilT) [Clostridium perfringens strain JXNC-3B]
MQSLAELLELTVKEGASDLHLTVGISPIIKVNGKLVRLEHEILRPEDTEAYAREILQDAYEKYDAIGEYDTSYSIHGKGR
FRVNIYKQRNSTALAIRVISLDMPTLDSLGYPETLKDICNLKRGLVLVTGPTGSGKSTTLAALINEINSNRESHIITIED
PIEFLHKHNKSIVNQREIGKDTLSYERALKAALREDPDVILIGEMRDLETISTAITAAETGHLVFSTLHTIGAAKTIDRI
VDVFPPHQQEQIKIQLASVLQIIISQQLVETVDGDRNAALEIMVATPAIKNLIREGKTHQIESSIQTGSKYGMRTMDMEL
ANLYREGIITQETAMNSAIDREILSRLLMY

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=716018 NQ194_RS10155 WP_003451114.1 2260118..2261170(-) (pilT) [Clostridium perfringens strain JXNC-3B]
ATGCAAAGTTTAGCAGAACTTTTAGAGTTAACAGTTAAAGAAGGAGCATCTGATTTGCATTTAACAGTAGGTATTTCTCC
TATAATAAAAGTTAATGGAAAATTAGTAAGGTTAGAACATGAAATATTACGCCCTGAAGATACTGAAGCGTATGCTAGAG
AGATATTACAGGATGCATATGAAAAGTATGATGCTATTGGAGAATACGATACATCATATTCAATACATGGTAAAGGTAGA
TTTAGAGTTAATATATATAAACAAAGAAATAGTACAGCTTTAGCTATAAGAGTTATATCTTTAGATATGCCAACTTTGGA
TAGTTTAGGATATCCTGAGACTTTAAAGGATATATGTAATCTTAAAAGAGGACTTGTATTAGTTACAGGACCTACTGGAA
GTGGTAAAAGTACAACTTTAGCAGCTTTAATAAATGAAATAAATAGTAATAGAGAATCTCATATAATAACAATAGAAGAT
CCTATAGAGTTTCTTCATAAACATAATAAATCTATTGTAAACCAAAGAGAGATAGGCAAGGACACTCTAAGTTATGAAAG
AGCCTTAAAAGCAGCTCTTAGAGAGGATCCTGATGTTATTTTAATTGGAGAAATGAGAGATTTAGAGACTATTTCAACAG
CTATAACAGCGGCAGAGACTGGACACTTAGTATTTTCAACTCTTCATACTATAGGAGCTGCTAAGACTATAGATAGAATA
GTAGATGTTTTTCCACCACATCAGCAGGAACAAATAAAAATACAGTTAGCTTCTGTTTTACAAATAATTATATCACAACA
ATTAGTGGAAACCGTTGATGGAGATAGAAATGCTGCATTAGAAATAATGGTTGCTACACCAGCTATAAAAAATTTAATAA
GAGAAGGAAAAACTCATCAAATAGAATCTTCTATCCAAACTGGAAGTAAATATGGAATGAGAACGATGGACATGGAACTT
GCTAATCTGTATAGAGAAGGTATAATAACACAAGAAACGGCAATGAATTCTGCTATAGATAGGGAAATTTTAAGTCGATT
ATTGATGTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A173YF66

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.711

98.857

0.491
  pilT Neisseria meningitidis 8013

49.709

98.286

0.489
  pilT Neisseria gonorrhoeae MS11

49.709

98.286

0.489
  pilT Pseudomonas aeruginosa PAK

50.298

96

0.483
  pilT Legionella pneumophila strain Lp02

49.415

97.714

0.483
  pilT Legionella pneumophila strain ERS1305867

49.415

97.714

0.483
  pilT Pseudomonas stutzeri DSM 10701

50

96

0.48
  pilT Acinetobacter baylyi ADP1

50.602

94.857

0.48
  pilT Acinetobacter nosocomialis M2

49.702

96

0.477
  pilT Acinetobacter baumannii D1279779

49.702

96

0.477
  pilT Acinetobacter baumannii strain A118

49.702

96

0.477
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

93.429

0.474
  pilT Vibrio cholerae strain A1552

50.765

93.429

0.474
  pilU Pseudomonas stutzeri DSM 10701

43.976

94.857

0.417
  pilU Vibrio cholerae strain A1552

40.653

96.286

0.391
  pilU Acinetobacter baylyi ADP1

39.823

96.857

0.386