Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   URS_RS08570 Genome accession   NZ_AP018824
Coordinates   1777132..1778169 (-) Length   345 a.a.
NCBI ID   WP_104794965.1    Uniprot ID   -
Organism   Acinetobacter ursingii strain M3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1772132..1783169
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  URS_RS08535 (URS_1740) - 1772904..1773353 (+) 450 WP_004991008.1 bacteriohemerythrin -
  URS_RS08545 (URS_1741) - 1773516..1774601 (+) 1086 WP_104794963.1 hypothetical protein -
  URS_RS08550 (URS_1742) - 1774598..1774918 (+) 321 WP_104794964.1 RnfH family protein -
  URS_RS08555 (URS_1743) - 1774962..1775360 (-) 399 WP_004991001.1 outer membrane protein assembly factor BamE -
  URS_RS08560 (URS_1744) fur 1775472..1775912 (+) 441 WP_004990998.1 ferric iron uptake transcriptional regulator -
  URS_RS08565 (URS_1745) pilU 1775986..1777104 (-) 1119 WP_004990996.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  URS_RS08570 (URS_1746) pilT 1777132..1778169 (-) 1038 WP_104794965.1 type IV pilus twitching motility protein PilT Machinery gene
  URS_RS08575 (URS_1747) - 1778293..1778979 (+) 687 WP_104794966.1 YggS family pyridoxal phosphate-dependent enzyme -
  URS_RS08580 (URS_1748) - 1779130..1779975 (+) 846 WP_004990991.1 undecaprenyl-diphosphate phosphatase -
  URS_RS08585 (URS_1749) - 1779933..1780624 (-) 692 Protein_1651 HAD family hydrolase -
  URS_RS08590 (URS_1751) - 1780705..1781490 (-) 786 WP_126624282.1 transglutaminase family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38359.98 Da        Isoelectric Point: 6.5052

>NTDB_id=71586 URS_RS08570 WP_104794965.1 1777132..1778169(-) (pilT) [Acinetobacter ursingii strain M3]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKEVHRLVYDIMNDKQRRDFEEQLETDFSFEVPNIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMDDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGMVSPQTARTVAKSPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=71586 URS_RS08570 WP_104794965.1 1777132..1778169(-) (pilT) [Acinetobacter ursingii strain M3]
ATGGATATCACAGAATTACTCGCCTTTTCGGTTAAAAACGGTGCATCAGATTTACATCTTTCCGCAGGAATGCCACCGAT
GATTCGTGTCGATGGTGAAGTCCGTCGTATTAATTTACCAGCACTGGAACACAAAGAAGTTCACCGTCTTGTTTATGACA
TTATGAATGATAAACAACGTCGTGACTTTGAAGAACAACTTGAAACTGACTTTTCCTTTGAAGTTCCAAACATTGCGCGT
TTTCGTGTCAATGCCTTTAACCAAAATCGTGGTGCAGGTGCAGTGTTTCGTACCATTCCGTCTAAAGTTCTCACCATGGA
TGATTTGGGTTTAGGGCAAATCTTTAAGGATATTTGTGATTATCCACGTGGTATTGTACTGGTTACAGGCCCAACGGGTT
CGGGTAAATCAACTACGCTTGCCGCCATGCTAGATTATATCAATGACAATCGCTATGACCATATTTTAACCGTAGAAGAT
CCGATCGAATTTGTACATCAATCCAAAAAGTGCTTGATTAACCAACGTGAAGTACATCGTGATACTCATGGCTTTAATGA
AGCACTACGTTCAGCACTACGTGAAGACCCTGATATCATCCTTGTCGGTGAGATGCGCGACCTTGAAACCATTCGTTTGG
CTTTGACCGCCGCAGAAACGGGTCACTTGGTATTTGGTACATTGCATACCACATCTGCTGCAAAAACCATTGACCGTGTA
ATTGACGTATTCCCTGCTGAAGAAAAAGATATGGTTCGTGCCATGTTATCTGAATCTTTACAAGCCGTTATTTCCCAAAC
ATTATTGAAGAAAAATGGTGGTGGCCGTGTCGCAGCGCATGAGATTATGATCGGGATACCTGCGATCCGTAACTTAATCC
GTGAAAATAAAGTCGCGCAAATGTACTCTGCTATTCAGACAGGTGCTAACTATGGCATGACCACGCTAGATCAAAGCTTG
AAAACGCTTGTGTCTAAAGGTATGGTTAGCCCACAGACAGCTCGTACTGTTGCCAAATCGCCCGAATCCTTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

96.232

100

0.962
  pilT Acinetobacter baumannii D1279779

95.942

100

0.959
  pilT Acinetobacter baumannii strain A118

95.942

100

0.959
  pilT Acinetobacter baylyi ADP1

88.372

99.71

0.881
  pilT Pseudomonas aeruginosa PAK

83.43

99.71

0.832
  pilT Pseudomonas stutzeri DSM 10701

82.849

99.71

0.826
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.345

97.391

0.675
  pilT Vibrio cholerae strain A1552

69.345

97.391

0.675
  pilT Neisseria meningitidis 8013

66.957

100

0.67
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.667
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.51
  pilU Vibrio cholerae strain A1552

41.056

98.841

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.469

98.841

0.4
  pilU Acinetobacter baylyi ADP1

39.306

100

0.394

Multiple sequence alignment