Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NQ527_RS04585 Genome accession   NZ_CP102270
Coordinates   929775..930836 (+) Length   353 a.a.
NCBI ID   WP_005603771.1    Uniprot ID   -
Organism   Eshraghiella crossota strain DSM 2876     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 924775..935836
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQ527_RS04565 (NQ527_04565) sigE 925223..925963 (+) 741 WP_005603776.1 RNA polymerase sporulation sigma factor SigE -
  NQ527_RS04570 (NQ527_04570) sigG 926017..926799 (+) 783 WP_005603775.1 RNA polymerase sporulation sigma factor SigG -
  NQ527_RS04575 (NQ527_04575) - 926862..928010 (+) 1149 WP_005603773.1 hypothetical protein -
  NQ527_RS04580 (NQ527_04580) - 928037..929752 (+) 1716 WP_005603772.1 GspE/PulE family protein -
  NQ527_RS04585 (NQ527_04585) pilT 929775..930836 (+) 1062 WP_005603771.1 type IV pilus twitching motility protein PilT Machinery gene
  NQ527_RS04590 (NQ527_04590) - 930934..932145 (+) 1212 WP_005603770.1 type II secretion system F family protein -
  NQ527_RS04595 (NQ527_04595) - 932259..932744 (+) 486 WP_052529872.1 type II secretion system protein -
  NQ527_RS04600 (NQ527_04600) - 932877..933644 (+) 768 WP_242648064.1 prepilin peptidase -
  NQ527_RS04605 (NQ527_04605) pilM 933693..935270 (+) 1578 WP_005603766.1 pilus assembly protein PilM -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 39210.43 Da        Isoelectric Point: 6.4344

>NTDB_id=715668 NQ527_RS04585 WP_005603771.1 929775..930836(+) (pilT) [Eshraghiella crossota strain DSM 2876]
MVTIEELLKIGKENGASDVHITVGLPPEMRVNGELMGMDYPRLTPTDTEEIVGQLLNEKQKNIIKEYGEADFSFSIPSLG
RYRVNAFKQRGSLAAAIRLVGTQIPRPEDLGLPKSVIELHNKKRGLILVTGPTGSGKSTTLASLINKINENRKAHIITLE
DPIEYLHTHKQAMINQREVGLDTMSYANALRAALREDPDVILVGEMRDLETISIAITAAETGHLVLSTLHTIGAASTIDR
IIDVFPPHQQQQIRIQLSMVLEAVISQQLIPTTDKHHRVAAFEVMLMTVAIKNLIRESKTFQIDSMIQTSREKGMITMDD
ALYELCMMGHITSESALSFAQDPVALEKKIKIY

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=715668 NQ527_RS04585 WP_005603771.1 929775..930836(+) (pilT) [Eshraghiella crossota strain DSM 2876]
ATGGTTACAATTGAAGAACTTTTAAAAATCGGTAAAGAAAACGGAGCATCCGACGTACATATTACGGTAGGACTCCCACC
TGAGATGAGAGTCAATGGTGAACTTATGGGAATGGATTACCCAAGGCTTACGCCAACAGACACAGAAGAAATAGTAGGCC
AGCTTCTTAATGAGAAGCAGAAAAACATCATTAAAGAATATGGAGAAGCAGATTTTTCATTTTCAATACCAAGCCTTGGA
CGATACAGGGTTAATGCATTTAAACAGCGTGGAAGTCTTGCGGCAGCAATCCGTCTGGTAGGAACACAGATTCCAAGACC
TGAAGATCTGGGACTTCCTAAATCAGTTATAGAATTACATAATAAGAAGAGAGGACTTATCCTCGTTACGGGACCTACCG
GAAGTGGTAAGTCTACTACACTTGCTTCATTAATTAATAAGATTAATGAGAATCGTAAAGCACATATTATTACACTTGAA
GATCCAATCGAGTACCTGCATACACATAAACAGGCGATGATTAACCAGAGAGAAGTCGGACTTGATACAATGAGTTATGC
CAATGCATTAAGGGCTGCACTCCGTGAAGATCCGGATGTCATCCTTGTAGGTGAGATGCGAGACCTTGAGACAATTTCGA
TAGCAATTACTGCAGCCGAGACAGGACATCTTGTTCTTTCAACATTGCATACAATAGGTGCGGCAAGTACAATTGACCGT
ATTATTGACGTATTCCCTCCTCATCAGCAGCAGCAGATAAGAATACAGCTCTCAATGGTACTTGAAGCGGTTATTTCACA
GCAGCTTATTCCTACAACGGATAAGCATCACCGTGTAGCAGCATTTGAAGTAATGCTTATGACAGTAGCAATTAAGAACC
TTATCCGTGAAAGCAAGACATTCCAGATTGATTCAATGATACAGACCAGCAGGGAAAAAGGCATGATAACAATGGATGAT
GCATTATATGAACTTTGTATGATGGGACATATTACGTCCGAATCCGCACTGAGCTTTGCACAGGACCCTGTTGCATTGGA
GAAAAAAATTAAAATATACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.17

96.884

0.496
  pilT Legionella pneumophila strain Lp02

51.327

96.034

0.493
  pilT Legionella pneumophila strain ERS1305867

51.327

96.034

0.493
  pilT Acinetobacter baumannii D1279779

50.588

96.317

0.487
  pilT Acinetobacter baumannii strain A118

50.588

96.317

0.487
  pilT Acinetobacter nosocomialis M2

50.588

96.317

0.487
  pilT Acinetobacter baylyi ADP1

49.706

96.317

0.479
  pilT Neisseria meningitidis 8013

49.263

96.034

0.473
  pilT Neisseria gonorrhoeae MS11

48.968

96.034

0.47
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.254

94.901

0.467
  pilT Vibrio cholerae strain A1552

49.254

94.901

0.467
  pilT Pseudomonas stutzeri DSM 10701

48.235

96.317

0.465
  pilT Pseudomonas aeruginosa PAK

48.235

96.317

0.465
  pilU Pseudomonas stutzeri DSM 10701

43.266

98.867

0.428
  pilU Acinetobacter baylyi ADP1

42.286

99.15

0.419
  pilU Vibrio cholerae strain A1552

42.769

92.068

0.394