Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NQ550_RS11245 Genome accession   NZ_CP102267
Coordinates   2429790..2430854 (-) Length   354 a.a.
NCBI ID   WP_025578213.1    Uniprot ID   -
Organism   Blautia wexlerae DSM 19850     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2424790..2435854
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQ550_RS11225 (NQ550_11225) - 2425688..2426452 (-) 765 WP_025578206.1 prepilin peptidase -
  NQ550_RS11230 (NQ550_11230) - 2426485..2427027 (-) 543 WP_025578208.1 type IV pilin protein -
  NQ550_RS11235 (NQ550_11235) - 2427124..2427627 (-) 504 WP_025578210.1 type IV pilin protein -
  NQ550_RS11240 (NQ550_11240) - 2428519..2429733 (-) 1215 WP_025578212.1 type II secretion system F family protein -
  NQ550_RS11245 (NQ550_11245) pilT 2429790..2430854 (-) 1065 WP_025578213.1 type IV pilus twitching motility protein PilT Machinery gene
  NQ550_RS11250 (NQ550_11250) - 2430961..2432655 (-) 1695 WP_025578215.1 GspE/PulE family protein -
  NQ550_RS11255 (NQ550_11255) - 2433013..2434881 (-) 1869 WP_242833558.1 C40 family peptidase -
  NQ550_RS11260 (NQ550_11260) - 2435197..2435787 (-) 591 WP_022381034.1 zinc dependent phospholipase C family protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39621.38 Da        Isoelectric Point: 6.1072

>NTDB_id=715631 NQ550_RS11245 WP_025578213.1 2429790..2430854(-) (pilT) [Blautia wexlerae DSM 19850]
MYNLDEILIQSVKVKASDIHLTTGRPPSYRIDGVLTPIEGERLTPQMLEDILMPLMDVRHREELQNNGQTDFAYAISGVG
RFRVNVFKQRGTLASVMRSLPFNIPEPEELGIPAEVVEMTTRKRGLILVTGPTGSGKSTTLASLIHVINRTYPYHIITLE
DPIEYLHRHDKSIVNQREIGSDSTNYAQALRAALREDPDVILVGEMRDLETISTAITAAETGHLVFSTLHTIGADKTIDR
IIDVFPPNQQQQIRIQLASVLECVVSQQLLKKADGSGRVAALEVLFANNAVRNLIRESKTYQIASIMQTNKRAGMQTMDD
ALYDLYMRKLIDADNAVTYAQDPVSMNKKVNFDF

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=715631 NQ550_RS11245 WP_025578213.1 2429790..2430854(-) (pilT) [Blautia wexlerae DSM 19850]
ATGTATAATTTAGACGAAATACTGATACAGTCAGTGAAGGTAAAGGCTTCCGATATCCATCTGACAACCGGTCGGCCGCC
AAGCTACAGGATCGATGGCGTACTGACCCCCATAGAGGGAGAACGCCTTACCCCGCAGATGCTTGAAGACATTCTCATGC
CCCTGATGGATGTCCGTCACAGAGAAGAATTACAGAACAATGGACAGACGGACTTCGCATATGCCATCTCAGGTGTGGGA
CGTTTTCGCGTAAATGTGTTTAAACAGAGAGGAACACTTGCTTCCGTTATGCGAAGCCTTCCTTTCAATATCCCGGAACC
TGAGGAGCTTGGTATTCCTGCAGAGGTAGTGGAGATGACCACGCGGAAAAGAGGTCTTATTTTGGTAACAGGGCCGACAG
GAAGCGGGAAATCAACTACATTGGCTTCATTGATCCATGTGATTAACAGGACGTATCCGTATCATATCATTACGCTGGAG
GACCCGATTGAATATCTGCACAGACATGATAAATCAATCGTAAACCAGAGAGAGATCGGAAGCGACTCAACAAATTATGC
GCAGGCGTTAAGGGCTGCACTTCGAGAGGATCCGGATGTTATCCTGGTAGGAGAGATGCGAGATCTGGAAACGATTTCTA
CTGCGATCACTGCGGCGGAGACCGGACATTTGGTATTTTCTACTTTGCATACGATTGGAGCAGATAAGACCATCGACCGA
ATCATCGATGTATTCCCTCCAAATCAGCAGCAGCAGATTCGTATCCAGCTTGCTTCGGTGTTGGAGTGTGTGGTATCACA
GCAGCTTCTTAAGAAAGCAGACGGAAGCGGCCGTGTGGCGGCACTGGAAGTACTTTTTGCAAATAATGCGGTGCGGAATC
TGATTCGTGAGAGTAAGACCTACCAGATCGCATCTATTATGCAGACCAATAAAAGAGCTGGTATGCAGACAATGGATGAT
GCGCTGTATGACTTATATATGAGAAAGCTTATCGATGCGGATAATGCAGTGACTTATGCGCAGGATCCGGTGAGCATGAA
TAAAAAAGTCAACTTTGATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.43

98.588

0.497
  pilT Neisseria meningitidis 8013

48.837

97.175

0.475
  pilT Legionella pneumophila strain Lp02

50.299

94.35

0.475
  pilT Legionella pneumophila strain ERS1305867

50.299

94.35

0.475
  pilT Neisseria gonorrhoeae MS11

48.547

97.175

0.472
  pilT Pseudomonas aeruginosa PAK

47.507

96.328

0.458
  pilT Pseudomonas stutzeri DSM 10701

47.507

96.328

0.458
  pilT Acinetobacter baumannii strain A118

46.802

97.175

0.455
  pilT Acinetobacter nosocomialis M2

46.802

97.175

0.455
  pilT Acinetobacter baumannii D1279779

46.802

97.175

0.455
  pilT Acinetobacter baylyi ADP1

46.512

97.175

0.452
  pilT Vibrio cholerae strain A1552

48.036

93.503

0.449
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.036

93.503

0.449
  pilU Acinetobacter baylyi ADP1

38.746

99.153

0.384
  pilU Pseudomonas stutzeri DSM 10701

39.466

95.198

0.376
  pilU Vibrio cholerae strain A1552

40.184

92.09

0.37