Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NQ230_RS04335 Genome accession   NZ_CP102247
Coordinates   885928..886908 (+) Length   326 a.a.
NCBI ID   WP_121424610.1    Uniprot ID   -
Organism   Enterobacter asburiae strain R_A5.MM     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 880928..891908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQ230_RS04295 (NQ230_04295) - 880995..881321 (+) 327 WP_014885198.1 YggL family protein -
  NQ230_RS04300 (NQ230_04300) - 881378..882094 (+) 717 WP_257260149.1 DUF2884 domain-containing protein -
  NQ230_RS04305 (NQ230_04305) - 882170..882532 (-) 363 WP_193940432.1 endonuclease domain-containing protein -
  NQ230_RS04310 (NQ230_04310) hemW 882596..883738 (-) 1143 WP_121424614.1 radical SAM family heme chaperone HemW -
  NQ230_RS04315 (NQ230_04315) - 883731..884324 (-) 594 WP_048979374.1 XTP/dITP diphosphatase -
  NQ230_RS04320 (NQ230_04320) yggU 884328..884624 (-) 297 WP_257260151.1 DUF167 family protein YggU -
  NQ230_RS04325 (NQ230_04325) - 884621..885187 (-) 567 WP_121424612.1 YggT family protein -
  NQ230_RS04330 (NQ230_04330) - 885209..885910 (-) 702 WP_257260154.1 YggS family pyridoxal phosphate-dependent enzyme -
  NQ230_RS04335 (NQ230_04335) pilT 885928..886908 (+) 981 WP_121424610.1 type IV pilus twitching motility protein PilT Machinery gene
  NQ230_RS04340 (NQ230_04340) ruvX 886919..887335 (-) 417 WP_029739223.1 Holliday junction resolvase RuvX -
  NQ230_RS04345 (NQ230_04345) - 887335..887895 (-) 561 WP_008499752.1 YqgE/AlgH family protein -
  NQ230_RS04350 (NQ230_04350) gshB 887983..888930 (-) 948 WP_159514863.1 glutathione synthase -
  NQ230_RS04355 (NQ230_04355) rsmE 888950..889681 (-) 732 WP_257261313.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NQ230_RS04360 (NQ230_04360) endA 889733..890440 (-) 708 WP_121424609.1 deoxyribonuclease I -
  NQ230_RS04365 (NQ230_04365) - 890535..891032 (-) 498 WP_045888327.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35413.38 Da        Isoelectric Point: 6.6087

>NTDB_id=715456 NQ230_RS04335 WP_121424610.1 885928..886908(+) (pilT) [Enterobacter asburiae strain R_A5.MM]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGTWWANGQVDFAATVTGGQRLRG
SAFKQMSGISVTLRLLPRTCPQLASLGAPRAIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNRHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFADALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=715456 NQ230_RS04335 WP_121424610.1 885928..886908(+) (pilT) [Enterobacter asburiae strain R_A5.MM]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGATTAGGCCGGCTTGAGCCTGCTCCCTTTCCACCTCCCGACGTTGGGGCGTTATTAAAAGCGTGGCTCAACG
ATGAGCAGCAGGGAACATGGTGGGCGAACGGGCAGGTGGACTTTGCCGCGACGGTGACGGGAGGCCAGCGTCTGCGCGGC
AGTGCCTTTAAGCAGATGAGCGGTATCTCTGTGACGCTGCGGCTGTTGCCGCGTACCTGCCCGCAGCTCGCTTCGCTGGG
CGCGCCGCGGGCAATCCCGGAACTGTTATCCAATGACGCCGGGCTGATTCTGGTGACAGGGGCAACCGGCAGCGGTAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTTAACCGCCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAGCGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCCGACGCGCTGCG
CAGTGCCTTACGCCAGGATCCGGACGTTATTCTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAGACCGGACATTTGGTGCTGGCGACGCTGCACACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAAGCTGCTTCC
CGATCTACAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACCGCAGCGGCGGCGAATCTTATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGCATCATCCAGACCGGTCAGCAGGCAGGAATGCAGAACTTTGACCAGAGTCTGGCGGAGAGA
CGGGCGCAGGGGCGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Legionella pneumophila strain ERS1305867

49.068

98.773

0.485
  pilT Legionella pneumophila strain Lp02

49.068

98.773

0.485
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii strain A118

47.401

100

0.475
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Acinetobacter baumannii D1279779

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

47.812

98.16

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.789

98.773

0.433
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362