Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NQ842_RS05030 Genome accession   NZ_CP102246
Coordinates   1050973..1051953 (+) Length   326 a.a.
NCBI ID   WP_014833224.1    Uniprot ID   -
Organism   Enterobacter cloacae complex sp. R_G8     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1045973..1056953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQ842_RS04990 (NQ842_04990) - 1046316..1046642 (+) 327 WP_003862421.1 YggL family protein -
  NQ842_RS04995 (NQ842_04995) - 1046699..1047415 (+) 717 WP_014833228.1 DUF2884 domain-containing protein -
  NQ842_RS05000 (NQ842_05000) - 1047513..1047599 (-) 87 Protein_968 DUF559 domain-containing protein -
  NQ842_RS05005 (NQ842_05005) hemW 1047635..1048783 (-) 1149 WP_014833227.1 radical SAM family heme chaperone HemW -
  NQ842_RS05010 (NQ842_05010) - 1048776..1049369 (-) 594 WP_013098669.1 XTP/dITP diphosphatase -
  NQ842_RS05015 (NQ842_05015) yggU 1049382..1049669 (-) 288 WP_083020673.1 DUF167 family protein YggU -
  NQ842_RS05020 (NQ842_05020) - 1049666..1050232 (-) 567 WP_013098667.1 YggT family protein -
  NQ842_RS05025 (NQ842_05025) - 1050254..1050955 (-) 702 WP_014833225.1 YggS family pyridoxal phosphate-dependent enzyme -
  NQ842_RS05030 (NQ842_05030) pilT 1050973..1051953 (+) 981 WP_014833224.1 type IV pilus twitching motility protein PilT Machinery gene
  NQ842_RS05035 (NQ842_05035) ruvX 1051964..1052380 (-) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  NQ842_RS05040 (NQ842_05040) - 1052380..1052940 (-) 561 WP_013098664.1 YqgE/AlgH family protein -
  NQ842_RS05045 (NQ842_05045) gshB 1053027..1053974 (-) 948 WP_046888609.1 glutathione synthase -
  NQ842_RS05050 (NQ842_05050) rsmE 1053994..1054725 (-) 732 WP_257256581.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NQ842_RS05055 (NQ842_05055) endA 1054778..1055485 (-) 708 WP_014833221.1 deoxyribonuclease I -
  NQ842_RS05060 (NQ842_05060) - 1055580..1056077 (-) 498 WP_046888678.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35640.60 Da        Isoelectric Point: 6.3038

>NTDB_id=715434 NQ842_RS05030 WP_014833224.1 1050973..1051953(+) (pilT) [Enterobacter cloacae complex sp. R_G8]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRAGRLEPAPFPSPDVDALLKTWLNDEQQGTWWASGQVDFAVTLAGNQRLRA
SAFKQMKGNSITLRLLPRVCPQLSALGAPQAIPELLSHDSGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FVYQSERCLIQQREIGLHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDRSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=715434 NQ842_RS05030 WP_014833224.1 1050973..1051953(+) (pilT) [Enterobacter cloacae complex sp. R_G8]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGAGCAGGCCGCCTTGAACCGGCTCCCTTTCCCTCTCCCGATGTGGATGCGTTACTGAAAACGTGGCTCAACG
ATGAGCAGCAGGGGACATGGTGGGCAAGCGGGCAGGTAGATTTTGCTGTGACCCTTGCGGGAAACCAGCGCCTGCGCGCA
AGCGCCTTTAAACAGATGAAGGGCAACTCCATCACGCTGCGCCTGTTACCCCGGGTCTGCCCGCAGCTCTCCGCCCTGGG
CGCTCCCCAGGCTATCCCGGAACTCCTGTCCCACGACAGCGGGCTGATACTGGTGACAGGCGCAACCGGCAGCGGCAAAT
CCACAACCCTTGCGGCAATGGTGGATTTTCTTAATCACCAGACGGATGGGCATATTCTGACGCTCGAAGATCCGGTGGAG
TTTGTCTATCAGAGCGAACGTTGTCTGATTCAGCAGCGGGAGATCGGCCTGCACAGTCCCTCATTTGCCGAGGCGCTGCG
CAGTGCGTTGCGTGAAGACCCCGATGTCATCCTGCTGGGAGAACTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACCG
CCGCTGAAACCGGGCATCTGGTGCTGGCGACGCTGCACACCCGCGGCGCCTCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGACCCGGTGCGTAATCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAGGCTACTCCC
CGACCTGCAGGGTGGGCGCGTCGCGCTGTATGAGCTGCTGGTGAATACGCCAGCGGCAGCGAACCTTATCCGCGAAGGTA
AAACGTGGCAGTTGCCCGGTATTATTCAAACCGGTCAGCAGGCGGGGATGCAGAATTTTGATCGGAGCCTGGCAGAGCGA
CGGGCGCAGGGGCGGTTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Pseudomonas stutzeri DSM 10701

48.93

100

0.491
  pilT Legionella pneumophila strain ERS1305867

49.379

98.773

0.488
  pilT Legionella pneumophila strain Lp02

49.379

98.773

0.488
  pilT Acinetobacter baumannii D1279779

48.318

100

0.485
  pilT Acinetobacter baumannii strain A118

48.318

100

0.485
  pilT Pseudomonas aeruginosa PAK

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Acinetobacter nosocomialis M2

48.75

98.16

0.479
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.168

98.773

0.426
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilB Haemophilus influenzae 86-028NP

33.708

100

0.368