Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LOY40_RS27305 Genome accession   NZ_CP102179
Coordinates   6052190..6053224 (-) Length   344 a.a.
NCBI ID   WP_030137909.1    Uniprot ID   A0A7V8UBG9
Organism   Pseudomonas alvandae strain B21-020     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6047190..6058224
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY40_RS27275 (LOY40_27275) ruvX 6047326..6047763 (+) 438 WP_030137904.1 Holliday junction resolvase RuvX -
  LOY40_RS27280 (LOY40_27280) pyrR 6047847..6048353 (+) 507 WP_030137905.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  LOY40_RS27285 (LOY40_27285) - 6048378..6049382 (+) 1005 WP_003177495.1 aspartate carbamoyltransferase catalytic subunit -
  LOY40_RS27290 (LOY40_27290) - 6049379..6050650 (+) 1272 WP_030137906.1 dihydroorotase -
  LOY40_RS27295 (LOY40_27295) - 6050774..6051202 (-) 429 WP_030137907.1 TM2 domain-containing protein -
  LOY40_RS27300 (LOY40_27300) - 6051451..6052062 (+) 612 WP_030137908.1 C40 family peptidase -
  LOY40_RS27305 (LOY40_27305) pilT 6052190..6053224 (-) 1035 WP_030137909.1 type IV pilus twitching motility protein PilT Machinery gene
  LOY40_RS27310 (LOY40_27310) - 6053281..6053973 (+) 693 WP_198796878.1 YggS family pyridoxal phosphate-dependent enzyme -
  LOY40_RS27315 (LOY40_27315) proC 6054002..6054820 (+) 819 WP_198796879.1 pyrroline-5-carboxylate reductase -
  LOY40_RS27320 (LOY40_27320) - 6054831..6055421 (+) 591 WP_198796880.1 YggT family protein -
  LOY40_RS27325 (LOY40_27325) - 6055694..6056833 (+) 1140 WP_030137913.1 homoserine O-acetyltransferase -
  LOY40_RS27330 (LOY40_27330) metW 6056841..6057461 (+) 621 WP_198796881.1 methionine biosynthesis protein MetW -
  LOY40_RS27335 (LOY40_27335) - 6057487..6057921 (+) 435 WP_198796882.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38178.80 Da        Isoelectric Point: 6.9777

>NTDB_id=715313 LOY40_RS27305 WP_030137909.1 6052190..6053224(-) (pilT) [Pseudomonas alvandae strain B21-020]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDRQRVDYEKFLETDFSFDVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKITEAPRGLVLVTGPTGSGKSTTLAAMIDYLNSHKHHHILTIED
PIEFVHEPRKCLINQREVHRDTQGFSTALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLQAVISQTLVKKIGGGRVAAHEIMLGTSAIRNLIREDKIAQMYSSIQTGGSLGMQTLDMCL
KDLVNKGLISREHARERARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=715313 LOY40_RS27305 WP_030137909.1 6052190..6053224(-) (pilT) [Pseudomonas alvandae strain B21-020]
ATGGATATCACTGAACTGTTGGCCTTCAGCGCCAAACAGGGGGCGTCGGACCTGCACCTGTCCGCCGGGCTGCCGCCGAT
GATCCGTGTCGACGGCGATGTGCGACGGATAAACCTGCCGGCACTGGACCATAAACAGGTTCATGAGCTGATCTACGACA
TCATGAACGACCGGCAGCGAGTGGATTACGAGAAATTTCTGGAAACGGATTTCTCCTTCGACGTGCCGGGTGTGGCGCGG
TTCAGGGTCAACGCCTTCAATCAGAATCGTGGCGCCGGGGCGGTGTTCCGGACCATCCCGTCGAAAGTCCTGACCATGGA
AGACCTGGGCATGGGCGAGGTGTTTCGCAAGATCACCGAGGCCCCACGCGGGCTCGTACTGGTGACCGGGCCTACTGGTT
CAGGCAAGTCCACGACCCTGGCGGCGATGATCGATTACCTGAATAGCCACAAGCACCATCACATCCTTACCATCGAGGAC
CCGATCGAGTTCGTCCATGAACCGCGCAAATGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCAAGGTTTTTCCAC
GGCCCTGCGCTCGGCCCTGCGGGAAGACCCGGACGTGATCCTGGTGGGGGAAATGCGTGACCTGGAAACCATTCGCCTGG
CGCTGACCGCCGCTGAAACCGGGCACTTGGTGTTTGGTACGCTGCACACCACATCGGCAGCCAAGACCATTGACCGGGTG
GTGGATGTGTTCCCCGGCGACGAAAAGTCCATGGTTCGCTCGATGCTATCGGAGTCGCTTCAAGCGGTGATCTCCCAGAC
GCTGGTCAAGAAGATCGGTGGCGGCCGGGTCGCTGCACACGAAATCATGCTCGGCACCTCGGCGATCCGCAATCTCATCC
GGGAAGACAAGATCGCGCAGATGTATTCGTCGATCCAGACCGGCGGCTCGCTGGGGATGCAGACGCTGGACATGTGTTTG
AAGGACTTGGTGAACAAGGGGCTGATCAGCCGCGAACATGCGCGGGAGCGGGCGCGTACGCCGGATAACTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7V8UBG9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.953

100

0.89
  pilT Pseudomonas aeruginosa PAK

88.081

100

0.881
  pilT Acinetobacter nosocomialis M2

79.651

100

0.797
  pilT Acinetobacter baumannii D1279779

79.651

100

0.797
  pilT Acinetobacter baumannii strain A118

79.651

100

0.797
  pilT Acinetobacter baylyi ADP1

78.779

100

0.788
  pilT Legionella pneumophila strain Lp02

73.373

98.256

0.721
  pilT Legionella pneumophila strain ERS1305867

73.373

98.256

0.721
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Vibrio cholerae strain A1552

39.71

100

0.398
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395