Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LOY71_RS24935 Genome accession   NZ_CP102176
Coordinates   5721890..5722924 (-) Length   344 a.a.
NCBI ID   WP_047702445.1    Uniprot ID   A0AAX2D7B2
Organism   Pseudomonas mediterranea strain B21-060     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5716890..5727924
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY71_RS24905 (LOY71_24910) ruvX 5717049..5717486 (+) 438 WP_047702440.1 Holliday junction resolvase RuvX -
  LOY71_RS24910 (LOY71_24915) pyrR 5717569..5718075 (+) 507 WP_047702441.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  LOY71_RS24915 (LOY71_24920) - 5718100..5719104 (+) 1005 WP_003177495.1 aspartate carbamoyltransferase catalytic subunit -
  LOY71_RS24920 (LOY71_24925) - 5719101..5720372 (+) 1272 WP_265086333.1 dihydroorotase -
  LOY71_RS24925 (LOY71_24930) - 5720478..5720906 (-) 429 WP_047702443.1 TM2 domain-containing protein -
  LOY71_RS24930 (LOY71_24935) - 5721157..5721768 (+) 612 WP_047702444.1 C40 family peptidase -
  LOY71_RS24935 (LOY71_24940) pilT 5721890..5722924 (-) 1035 WP_047702445.1 type IV pilus twitching motility protein PilT Machinery gene
  LOY71_RS24940 (LOY71_24945) - 5722980..5723672 (+) 693 WP_201868696.1 YggS family pyridoxal phosphate-dependent enzyme -
  LOY71_RS24945 (LOY71_24950) proC 5723709..5724527 (+) 819 WP_047702447.1 pyrroline-5-carboxylate reductase -
  LOY71_RS24950 (LOY71_24955) - 5724538..5725128 (+) 591 WP_047702448.1 YggT family protein -
  LOY71_RS24955 (LOY71_24960) - 5725337..5726476 (+) 1140 WP_047702449.1 homoserine O-acetyltransferase -
  LOY71_RS24960 (LOY71_24965) metW 5726484..5727104 (+) 621 WP_041023980.1 methionine biosynthesis protein MetW -
  LOY71_RS24965 (LOY71_24970) - 5727133..5727567 (+) 435 WP_055127827.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38122.78 Da        Isoelectric Point: 6.9777

>NTDB_id=715254 LOY71_RS24935 WP_047702445.1 5721890..5722924(-) (pilT) [Pseudomonas mediterranea strain B21-060]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDRQRVDYEKFLETDFSFDVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMDELGMGEVFRKVTEAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHKHHHILTIED
PIEFVHEPRKCLINQREVHRDTQGFSTALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLQAVISQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVTKGLISREHARERARTPDSF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=715254 LOY71_RS24935 WP_047702445.1 5721890..5722924(-) (pilT) [Pseudomonas mediterranea strain B21-060]
ATGGATATCACTGAACTGCTGGCGTTCAGCGCCAAACAGGGGGCTTCGGACCTGCACCTGTCTGCCGGGTTGCCGCCGAT
GATCCGGGTTGATGGCGATGTGCGGCGGATCAACCTGCCGGCACTGGACCACAAACAGGTTCATGAACTGATCTACGACA
TCATGAACGACCGGCAACGGGTAGATTACGAAAAGTTTCTGGAAACCGATTTTTCCTTCGATGTACCCGGGGTCGCACGG
TTCCGGGTCAACGCGTTCAATCAGAATCGCGGCGCGGGGGCGGTGTTCCGGACCATTCCGTCGAAAGTCCTGACCATGGA
CGAGCTGGGCATGGGCGAGGTGTTTCGCAAAGTGACCGAGGCTCCCCGCGGGTTGGTGCTGGTGACGGGGCCGACCGGCT
CAGGCAAGTCCACCACCCTGGCGGCGATGATCGACTACCTCAATACCCATAAGCACCACCACATCCTCACCATCGAGGAC
CCCATCGAGTTTGTCCACGAACCGCGCAAATGCCTGATCAACCAGCGCGAAGTGCACCGCGACACCCAAGGCTTTTCCAC
GGCCCTGCGCTCGGCCCTGCGGGAAGACCCGGACGTGATCCTGGTGGGGGAGATGCGCGACCTGGAAACCATCCGCCTGG
CACTGACCGCCGCCGAAACCGGGCACCTGGTCTTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGGGTG
GTGGACGTGTTCCCGGGAGACGAGAAGTCCATGGTGCGCTCGATGTTGTCGGAATCATTGCAGGCGGTGATTTCCCAGAC
GCTGATCAAGAAGATCGGCGGCGGCCGGGTCGCCGCTCATGAAATCATGCTGGGGACGTCGGCGATTCGTAACCTGATCC
GCGAAGACAAAGTGGCGCAGATGTACTCGGCGATCCAGACCGGAGGCTCGCTGGGGATGCAGACGCTGGACATGTGTCTG
AAGGATCTGGTGACCAAGGGCTTGATCAGCCGTGAACATGCGCGGGAACGGGCGCGTACGCCGGATAGTTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

88.953

100

0.89
  pilT Pseudomonas stutzeri DSM 10701

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

79.651

100

0.797
  pilT Acinetobacter baumannii D1279779

79.651

100

0.797
  pilT Acinetobacter baumannii strain A118

79.651

100

0.797
  pilT Acinetobacter baylyi ADP1

78.779

100

0.788
  pilT Legionella pneumophila strain Lp02

70.64

100

0.706
  pilT Legionella pneumophila strain ERS1305867

70.64

100

0.706
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.965

0.669
  pilT Vibrio cholerae strain A1552

68.249

97.965

0.669
  pilT Neisseria meningitidis 8013

66.087

100

0.663
  pilT Neisseria gonorrhoeae MS11

65.797

100

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398
  pilU Vibrio cholerae strain A1552

40.597

97.384

0.395
  pilU Pseudomonas stutzeri DSM 10701

40

97.384

0.39