Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LOY65_RS25615 Genome accession   NZ_CP102175
Coordinates   5815932..5816966 (-) Length   344 a.a.
NCBI ID   WP_265068352.1    Uniprot ID   -
Organism   Pseudomonas corrugata strain B21-061     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5810932..5821966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY65_RS25585 (LOY65_25590) ruvX 5811155..5811592 (+) 438 WP_024778885.1 Holliday junction resolvase RuvX -
  LOY65_RS25590 (LOY65_25595) pyrR 5811674..5812180 (+) 507 WP_024778886.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  LOY65_RS25595 (LOY65_25600) - 5812205..5813209 (+) 1005 WP_024778887.1 aspartate carbamoyltransferase catalytic subunit -
  LOY65_RS25600 (LOY65_25605) - 5813206..5814477 (+) 1272 WP_024778888.1 dihydroorotase -
  LOY65_RS25605 (LOY65_25610) - 5814600..5815028 (-) 429 WP_024778889.1 TM2 domain-containing protein -
  LOY65_RS25610 (LOY65_25615) - 5815278..5815889 (+) 612 WP_024778890.1 C40 family peptidase -
  LOY65_RS25615 (LOY65_25620) pilT 5815932..5816966 (-) 1035 WP_265068352.1 type IV pilus twitching motility protein PilT Machinery gene
  LOY65_RS25620 (LOY65_25625) - 5817022..5817714 (+) 693 WP_265075454.1 YggS family pyridoxal phosphate-dependent enzyme -
  LOY65_RS25625 (LOY65_25630) proC 5817751..5818569 (+) 819 WP_024778893.1 pyrroline-5-carboxylate reductase -
  LOY65_RS25630 (LOY65_25635) - 5818580..5819170 (+) 591 WP_024778894.1 YggT family protein -
  LOY65_RS25635 (LOY65_25640) - 5819353..5820492 (+) 1140 WP_024778895.1 homoserine O-acetyltransferase -
  LOY65_RS25640 (LOY65_25645) metW 5820500..5821120 (+) 621 WP_053190564.1 methionine biosynthesis protein MetW -
  LOY65_RS25645 (LOY65_25650) - 5821149..5821583 (+) 435 WP_024778897.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38135.78 Da        Isoelectric Point: 6.9777

>NTDB_id=715225 LOY65_RS25615 WP_265068352.1 5815932..5816966(-) (pilT) [Pseudomonas corrugata strain B21-061]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDRQRVDYEKFLETDFSFDVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKVTEAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHKHHHILTIED
PIEFVHEPRKCLINQREVHRDTQGFSTALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLQAVISQTLVKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVTKGLISREHARERARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=715225 LOY65_RS25615 WP_265068352.1 5815932..5816966(-) (pilT) [Pseudomonas corrugata strain B21-061]
ATGGATATCACTGAACTGCTGGCGTTCAGCGCCAAACAGGGAGCGTCGGACCTGCACTTGTCCGCTGGGCTGCCACCGAT
GATCCGGGTCGATGGCGATGTGCGGCGGATCAACCTGCCGGCACTGGACCATAAACAGGTTCATGAACTGATCTACGACA
TCATGAACGACCGGCAACGGGTAGATTACGAAAAGTTTCTGGAAACCGATTTTTCCTTCGATGTACCCGGGGTCGCACGG
TTCCGGGTCAACGCGTTCAATCAGAATCGCGGCGCGGGGGCGGTATTCCGGACCATCCCGTCGAAAGTCCTGACCATGGA
AGATCTGGGCATGGGCGAGGTTTTTCGCAAAGTGACCGAGGCCCCCCGCGGGCTGGTGCTGGTCACCGGGCCGACCGGCT
CAGGCAAATCCACCACCCTCGCGGCGATGATCGACTACCTCAACACCCATAAGCACCACCACATCCTCACCATCGAGGAC
CCTATCGAGTTTGTCCACGAGCCGCGCAAATGCCTGATCAACCAGCGCGAAGTGCACCGCGACACCCAAGGTTTTTCCAC
AGCCTTGCGTTCGGCCCTGCGGGAAGACCCTGACGTGATCCTGGTGGGGGAGATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCCGAAACCGGGCACCTGGTATTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGGGTG
GTGGACGTGTTTCCGGGGGACGAAAAGTCCATGGTGCGCTCGATGTTGTCGGAATCATTACAAGCGGTGATTTCCCAGAC
GCTGGTCAAGAAGATCGGCGGCGGCCGGGTCGCCGCTCATGAAATCATGCTGGGCACGTCGGCGATCCGTAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCGGCTCGCTGGGGATGCAGACGCTGGACATGTGCTTG
AAGGACCTGGTGACCAAGGGATTGATCAGCCGGGAGCACGCGCGGGAGCGGGCGCGTACGCCGGATAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

88.953

100

0.89
  pilT Pseudomonas stutzeri DSM 10701

88.663

100

0.887
  pilT Acinetobacter nosocomialis M2

79.942

100

0.799
  pilT Acinetobacter baumannii D1279779

79.942

100

0.799
  pilT Acinetobacter baumannii strain A118

79.942

100

0.799
  pilT Acinetobacter baylyi ADP1

78.488

100

0.785
  pilT Legionella pneumophila strain Lp02

72.189

98.256

0.709
  pilT Legionella pneumophila strain ERS1305867

72.189

98.256

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

65.797

100

0.66
  pilT Neisseria gonorrhoeae MS11

65.507

100

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Vibrio cholerae strain A1552

39.71

100

0.398
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398