Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGF   Type   Machinery gene
Locus tag   M0P28_RS00805 Genome accession   NZ_CP116958
Coordinates   120216..120683 (+) Length   155 a.a.
NCBI ID   WP_058692632.1    Uniprot ID   -
Organism   Streptococcus pasteurianus strain WUSP074     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 115216..125683
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0P28_RS00775 (M0P28_00775) - 116966..117328 (+) 363 WP_058692629.1 DUF1033 family protein -
  M0P28_RS00780 (M0P28_00780) comGA 117399..118340 (+) 942 WP_003062917.1 competence type IV pilus ATPase ComGA Machinery gene
  M0P28_RS00785 (M0P28_00785) comGB 118264..119307 (+) 1044 WP_003062919.1 competence type IV pilus assembly protein ComGB Machinery gene
  M0P28_RS00790 (M0P28_00790) comGC 119307..119600 (+) 294 WP_117479472.1 competence type IV pilus major pilin ComGC Machinery gene
  M0P28_RS00795 (M0P28_00795) comGD 119584..120015 (+) 432 WP_058692630.1 competence type IV pilus minor pilin ComGD Machinery gene
  M0P28_RS00800 (M0P28_00800) comGE 119969..120262 (+) 294 WP_081048608.1 competence type IV pilus minor pilin ComGE Machinery gene
  M0P28_RS00805 (M0P28_00805) comGF 120216..120683 (+) 468 WP_058692632.1 competence type IV pilus minor pilin ComGF Machinery gene
  M0P28_RS00810 (M0P28_00810) comGG 120637..120987 (+) 351 WP_238374707.1 competence type IV pilus minor pilin ComGG Machinery gene
  M0P28_RS00815 (M0P28_00815) comGH 121042..122013 (+) 972 WP_117479470.1 class I SAM-dependent methyltransferase Machinery gene
  M0P28_RS00820 (M0P28_00820) - 122051..123250 (+) 1200 WP_058692635.1 acetate kinase -
  M0P28_RS00825 (M0P28_00825) - 123417..123617 (+) 201 WP_058692636.1 helix-turn-helix transcriptional regulator -
  M0P28_RS00830 (M0P28_00830) - 123627..123833 (+) 207 WP_058692637.1 hypothetical protein -
  M0P28_RS00835 (M0P28_00835) - 123824..124276 (+) 453 WP_117479468.1 ABC transporter permease -
  M0P28_RS00840 (M0P28_00840) - 124288..124923 (+) 636 WP_058692639.1 CPBP family intramembrane glutamic endopeptidase -

Sequence


Protein


Download         Length: 155 a.a.        Molecular weight: 17515.10 Da        Isoelectric Point: 9.7290

>NTDB_id=715225 M0P28_RS00805 WP_058692632.1 120216..120683(+) (comGF) [Streptococcus pasteurianus strain WUSP074]
MFMRGKRKYYTLKKTSLKAFTLIECLVSLLIISGAILVYNGLTQSISANVHYLSENQEENWLLFSQQLRAELANCQLDKV
ENNKLYVTKSSQKLAFGQSKADDFRKTNASGQGYQPMIFGVKSSAISRDGQKVTMTLNLENGLERTFVYTFETAS

Nucleotide


Download         Length: 468 bp        

>NTDB_id=715225 M0P28_RS00805 WP_058692632.1 120216..120683(+) (comGF) [Streptococcus pasteurianus strain WUSP074]
ATGTTTATGAGGGGAAAACGGAAATATTACACGTTAAAAAAGACTAGTTTAAAGGCATTCACCCTCATAGAATGTTTGGT
TTCTTTATTGATAATTTCGGGTGCTATTCTTGTTTACAATGGCTTAACGCAATCTATTTCTGCAAATGTGCATTATTTGT
CGGAAAATCAAGAGGAAAACTGGCTTTTGTTTTCACAACAGCTGCGTGCGGAACTTGCCAATTGTCAATTAGATAAGGTT
GAAAATAACAAGCTATATGTGACGAAGTCCAGTCAAAAGTTGGCATTTGGACAGTCAAAGGCTGATGATTTTCGTAAAAC
AAATGCATCTGGTCAAGGCTATCAGCCAATGATATTTGGAGTAAAATCTTCTGCTATTTCTAGAGATGGTCAGAAGGTGA
CAATGACATTGAATTTAGAGAATGGTTTGGAGAGGACATTTGTTTACACTTTTGAAACAGCAAGTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Streptococcus mutans UA140

57.554

89.677

0.516

  comGF Streptococcus mutans UA159

56.835

89.677

0.51

  comGF/cglF Streptococcus pneumoniae Rx1

45

90.323

0.406

  comGF/cglF Streptococcus pneumoniae D39

45

90.323

0.406

  comGF/cglF Streptococcus pneumoniae R6

45

90.323

0.406

  comGF/cglF Streptococcus pneumoniae TIGR4

45

90.323

0.406

  comGF/cglF Streptococcus mitis NCTC 12261

43.662

91.613

0.4

  comGF/cglF Streptococcus mitis SK321

42.958

91.613

0.394

  comGF Lactococcus lactis subsp. cremoris KW2

44.203

89.032

0.394