Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NP224_RS04205 Genome accession   NZ_CP102103
Coordinates   857415..858395 (+) Length   326 a.a.
NCBI ID   WP_064358264.1    Uniprot ID   A0AAX3CSN2
Organism   Klebsiella michiganensis strain BSI-KPN166     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 852415..863395
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NP224_RS04175 (NP224_04175) - 852797..853783 (-) 987 WP_064377969.1 TRAP transporter substrate-binding protein -
  NP224_RS04180 (NP224_04180) hemW 854088..855224 (-) 1137 WP_064377970.1 radical SAM family heme chaperone HemW -
  NP224_RS04185 (NP224_04185) - 855217..855810 (-) 594 WP_004854157.1 XTP/dITP diphosphatase -
  NP224_RS04190 (NP224_04190) yggU 855823..856113 (-) 291 WP_004854155.1 DUF167 family protein YggU -
  NP224_RS04195 (NP224_04195) - 856110..856676 (-) 567 WP_004105714.1 YggT family protein -
  NP224_RS04200 (NP224_04200) - 856694..857398 (-) 705 WP_064358265.1 YggS family pyridoxal phosphate-dependent enzyme -
  NP224_RS04205 (NP224_04205) pilT 857415..858395 (+) 981 WP_064358264.1 type IV pilus twitching motility protein PilT Machinery gene
  NP224_RS04210 (NP224_04210) - 858744..859397 (+) 654 WP_064358263.1 LuxR C-terminal-related transcriptional regulator -
  NP224_RS04215 (NP224_04215) ruvX 859453..859869 (-) 417 WP_014226836.1 Holliday junction resolvase RuvX -
  NP224_RS04220 (NP224_04220) - 859869..860432 (-) 564 WP_014226835.1 YqgE/AlgH family protein -
  NP224_RS04225 (NP224_04225) gshB 860609..861556 (-) 948 WP_014226834.1 glutathione synthase -
  NP224_RS04230 (NP224_04230) rsmE 861577..862308 (-) 732 WP_025105941.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NP224_RS04235 (NP224_04235) endA 862400..863107 (-) 708 WP_014226832.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35236.46 Da        Isoelectric Point: 6.6867

>NTDB_id=714422 NP224_RS04205 WP_064358264.1 857415..858395(+) (pilT) [Klebsiella michiganensis strain BSI-KPN166]
MELEEIVALSVKHNVSDLHLCNASTPRWRRQGKLEPAPFTSPDIGKILADWLNAEQLAQWQACGQVDFALAPAGGPRLRA
SAFVHNRGLSLALRLLAETCPQLADLGAPAALTELLNEESGLILVTGATGSGKSTTLAAMVGYLNQRLNGHILTLEDPVE
FIHRSERCLIQQREIGRHCPSFAAALRAALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAQAVERLVDV
FPAQEKEQVRSQLAGSLCAVLAQKLFPDTAGGRVALYELLVNTPAVANLIREGKTYQLPGVMQTGQQFGMQTFTQSFQQR
TAAGLL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=714422 NP224_RS04205 WP_064358264.1 857415..858395(+) (pilT) [Klebsiella michiganensis strain BSI-KPN166]
ATGGAACTGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAATGCTTCAACGCCGCG
CTGGCGTCGCCAGGGCAAGCTGGAGCCTGCGCCTTTTACCTCTCCGGATATTGGCAAGATACTCGCTGATTGGCTCAACG
CCGAGCAGCTCGCCCAGTGGCAGGCCTGCGGACAGGTCGATTTCGCGCTGGCTCCGGCCGGCGGCCCGCGCCTGCGCGCC
AGCGCATTTGTCCACAACCGCGGCCTGTCGCTGGCCCTGCGTCTGCTGGCGGAAACCTGTCCGCAGCTGGCGGATCTCGG
CGCGCCGGCCGCCTTAACGGAACTGCTCAATGAGGAGAGCGGGCTTATCCTGGTCACGGGCGCCACCGGTAGCGGGAAAT
CCACCACCCTGGCGGCGATGGTGGGCTATCTTAATCAGCGCCTCAATGGCCATATCCTGACGCTGGAAGATCCGGTTGAG
TTTATTCACCGCAGCGAGCGCTGCCTGATCCAGCAGCGGGAAATCGGTCGCCACTGCCCCTCATTTGCCGCGGCCCTGCG
CGCTGCGCTGCGTCAGGACCCGGATGTCATTCTGCTAGGGGAACTGCGTGACAGCGAAACGATCCGCCTGGCACTGACCG
CGGCGGAGACCGGACATCTGGTTATGGCGACGCTGCATACGCGCGGCGCGGCACAGGCGGTTGAAAGACTGGTGGATGTT
TTTCCCGCTCAGGAGAAAGAGCAGGTTCGTAGCCAGCTGGCGGGAAGTTTATGCGCGGTGCTGGCACAAAAATTGTTTCC
GGATACTGCTGGCGGAAGAGTTGCGCTATACGAGCTTTTGGTTAATACCCCGGCGGTGGCGAATTTGATCCGCGAGGGGA
AAACGTATCAGCTGCCCGGCGTGATGCAAACCGGGCAGCAATTCGGGATGCAAACTTTTACGCAAAGCTTTCAGCAACGT
ACGGCTGCGGGTCTTTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.848

100

0.503
  pilT Vibrio cholerae strain A1552

49.848

100

0.503
  pilT Neisseria meningitidis 8013

48.182

100

0.488
  pilT Neisseria gonorrhoeae MS11

47.879

100

0.485
  pilT Legionella pneumophila strain Lp02

48.113

97.546

0.469
  pilT Legionella pneumophila strain ERS1305867

48.113

97.546

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.201

100

0.466
  pilT Acinetobacter baumannii strain A118

45.455

100

0.46
  pilT Acinetobacter baumannii D1279779

45.455

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.593

100

0.46
  pilT Acinetobacter nosocomialis M2

45.152

100

0.457
  pilT Acinetobacter baylyi ADP1

44.985

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.479

97.239

0.433
  pilU Pseudomonas stutzeri DSM 10701

40.854

100

0.411
  pilU Vibrio cholerae strain A1552

40.373

98.773

0.399
  pilU Acinetobacter baylyi ADP1

38.7

99.08

0.383