Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NP442_RS03985 Genome accession   NZ_CP101922
Coordinates   813500..814480 (+) Length   326 a.a.
NCBI ID   WP_098717180.1    Uniprot ID   -
Organism   Escherichia coli strain JCC-EE 7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 808500..819480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NP442_RS03955 (NP442_03950) yggM 809015..810022 (+) 1008 WP_000745217.1 DUF1202 family protein -
  NP442_RS03960 (NP442_03955) hemW 810177..811313 (-) 1137 WP_098717179.1 radical SAM family heme chaperone HemW -
  NP442_RS03965 (NP442_03960) rdgB 811306..811899 (-) 594 WP_001174735.1 XTP/dITP diphosphatase -
  NP442_RS03970 (NP442_03965) yggU 811907..812197 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  NP442_RS03975 (NP442_03970) yggT 812194..812760 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  NP442_RS03980 (NP442_03975) yggS 812778..813482 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  NP442_RS03985 (NP442_03980) pilT 813500..814480 (+) 981 WP_098717180.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NP442_RS03990 (NP442_03985) ruvX 814655..815071 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  NP442_RS03995 (NP442_03990) yqgE 815071..815634 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  NP442_RS04000 (NP442_03995) gshB 815743..816693 (-) 951 WP_000593273.1 glutathione synthase -
  NP442_RS04005 (NP442_04000) rsmE 816706..817437 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NP442_RS04010 (NP442_04005) endA 817517..818224 (-) 708 WP_000286517.1 deoxyribonuclease I -
  NP442_RS04015 (NP442_04010) yggI 818319..818816 (-) 498 WP_032142436.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35931.15 Da        Isoelectric Point: 5.5857

>NTDB_id=712923 NP442_RS03985 WP_098717180.1 813500..814480(+) (pilT) [Escherichia coli strain JCC-EE 7]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAVQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLICEGKTHQLPHVIQTGQQVGMITFQQSFQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=712923 NP442_RS03985 WP_098717180.1 813500..814480(+) (pilT) [Escherichia coli strain JCC-EE 7]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCATTCGCGCAACGGCAAGGCATTTCGCTGGCGTTACGGCTGTTGCCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGAAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAAATCGGTTTGCATTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGCGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGACACCTGGTACTGGCAACCTTACATACACGTGGTGCCGTGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCAGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTTGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTTTCAGCAGCGA
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

39.21

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362